Signal Transduction — Single Cell Techniques [electronic resource] /

Signal Transduction — Single Cell Techniques [electronic resource] /

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A variety of powerful techniques to monitor and analyse the events during signal transduction at the single cell level are described in this lab manual. An introductionary section for cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.

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Bibliographic Details
Main Authors: Duijn, Bert Van. editor., Wiltink, Anneke. editor., SpringerLink (Online service)
Format: Texto biblioteca
Language:eng
Published: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1998
Subjects:Life sciences., Pharmacology., Biochemistry., Cell biology., Biomedical engineering., Life Sciences., Cell Biology., Biomedical Engineering., Biochemistry, general., Pharmacology/Toxicology.,
Online Access:http://dx.doi.org/10.1007/978-3-642-80368-0
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id KOHA-OAI-TEST:180242
record_format koha
institution COLPOS
collection Koha
country México
countrycode MX
component Bibliográfico
access En linea
En linea
databasecode cat-colpos
tag biblioteca
region America del Norte
libraryname Departamento de documentación y biblioteca de COLPOS
language eng
topic Life sciences.
Pharmacology.
Biochemistry.
Cell biology.
Biomedical engineering.
Life Sciences.
Cell Biology.
Biomedical Engineering.
Biochemistry, general.
Pharmacology/Toxicology.
Life sciences.
Pharmacology.
Biochemistry.
Cell biology.
Biomedical engineering.
Life Sciences.
Cell Biology.
Biomedical Engineering.
Biochemistry, general.
Pharmacology/Toxicology.
spellingShingle Life sciences.
Pharmacology.
Biochemistry.
Cell biology.
Biomedical engineering.
Life Sciences.
Cell Biology.
Biomedical Engineering.
Biochemistry, general.
Pharmacology/Toxicology.
Life sciences.
Pharmacology.
Biochemistry.
Cell biology.
Biomedical engineering.
Life Sciences.
Cell Biology.
Biomedical Engineering.
Biochemistry, general.
Pharmacology/Toxicology.
Duijn, Bert Van. editor.
Wiltink, Anneke. editor.
SpringerLink (Online service)
Signal Transduction — Single Cell Techniques [electronic resource] /
description A variety of powerful techniques to monitor and analyse the events during signal transduction at the single cell level are described in this lab manual. An introductionary section for cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.
format Texto
topic_facet Life sciences.
Pharmacology.
Biochemistry.
Cell biology.
Biomedical engineering.
Life Sciences.
Cell Biology.
Biomedical Engineering.
Biochemistry, general.
Pharmacology/Toxicology.
author Duijn, Bert Van. editor.
Wiltink, Anneke. editor.
SpringerLink (Online service)
author_facet Duijn, Bert Van. editor.
Wiltink, Anneke. editor.
SpringerLink (Online service)
author_sort Duijn, Bert Van. editor.
title Signal Transduction — Single Cell Techniques [electronic resource] /
title_short Signal Transduction — Single Cell Techniques [electronic resource] /
title_full Signal Transduction — Single Cell Techniques [electronic resource] /
title_fullStr Signal Transduction — Single Cell Techniques [electronic resource] /
title_full_unstemmed Signal Transduction — Single Cell Techniques [electronic resource] /
title_sort signal transduction — single cell techniques [electronic resource] /
publisher Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer,
publishDate 1998
url http://dx.doi.org/10.1007/978-3-642-80368-0
work_keys_str_mv AT duijnbertvaneditor signaltransductionsinglecelltechniqueselectronicresource
AT wiltinkannekeeditor signaltransductionsinglecelltechniqueselectronicresource
AT springerlinkonlineservice signaltransductionsinglecelltechniqueselectronicresource
_version_ 1756264659354124288
spelling KOHA-OAI-TEST:1802422018-07-30T22:59:59ZSignal Transduction — Single Cell Techniques [electronic resource] / Duijn, Bert Van. editor. Wiltink, Anneke. editor. SpringerLink (Online service) textBerlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer,1998.engA variety of powerful techniques to monitor and analyse the events during signal transduction at the single cell level are described in this lab manual. An introductionary section for cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.I Handling of Cells in Single Cell Experiments -- 1 Maintaining Cells Under the Microscope -- 2 Simple Perfusion Chambers for Single Cell Measurements -- 3 Temperature Control on the Stage of the Microscope -- 4 A Modified U-Tube Drug Application System -- 5 Laser Microsurgery as a Tool in Single Cell Research -- II Ion Channel and Membrane Potential Measurements Using the Patch-Clamp Technique -- II–1 Introduction to the Measurement Technique -- 6 Significance of Ion Channels and Membrane Potential Changes in Cells -- 7 Practical Introduction to Patch Clamping by Simulation Experiments with Simple Electrical Circuits -- 8 Improved Electrophysiological Measurements by Series Resistance Compensation -- 9 Preparation of Patchable Plant Cell Protoplasts and a Procedure for the Improvement of Gigaseal Formation -- II–2 Single Channel Measurements -- 10 Recording and Analysis of ATP-Sensitive Potassium Channels in Inside-Out Patches of Rabbit Ventricular Myocytes -- 11 Mechanotransduction and Mechanosensitive Ion Channels in Osteoblasts -- 12 The Study of (Plant) Ion Channels Reconstituted in Planar Lipid Bilayers -- 13 Ion Channel Measurement on the Cell Nucleus -- 14 Monitoring Cytosolic Calcium and Membrane Potential from Cell-Attached Patch-Clamp Currents in Human T Lymphocytes -- II–3 Whole Cell Measurements -- 15 Membrane Potential and Action Potential Measurements in Whole Cell and Perforated Patch Configurations -- 16 Perforated Patch-Clamp Technique in Heart Cells -- 17 Measurement and Analysis of Different Aspects of Potassium Currents in Human Lymphocytes -- 18 Ionic Conductances in Chicken Osteoclasts -- 19 GABAA Receptor-Mediated Chloride Currents in Acutely Dissociated Hippocampal Neurons -- 20 Measurement of Whole Cell Potassium Currents in Protoplasts from Tobacco Cell Suspensions -- 21 Inward Rectifying Potassium Conductance in Barley Aleurone Protoplasts -- III Fluorescence to Measure Intracellular Ions -- III–1 Introduction to the Measurement Technique -- 22 An Introduction to the Use of Fluorescent Probes in Ratiometric Fluorescence Microscopy -- 23 How To Use Video Cameras To Acquire Images -- 24 Strategies for Studying Intracellular pH Regulation -- III–2 Flow Cytometer Measurements -- 25 An Introduction to the Working Principles of the Flow Cytometer -- 26 Flow Cytometric Membrane Potential Measurements -- 27 Flow Cytometric Measurement of Cytosolic Calcium in Lymphocytes -- III–3 Microfluorescence Measurements -- 28 Cytosolic pH and Cell Movement Measurement in Dictyostelium -- 29 Analysis of Agonist-Induced Cell Recruitment in Terms of Intracellular Calcium Mobilization in a Population of Enzymatically Dispersed Pancreatic Acinar Cells -- III–4 Confocal Microscopy -- 30 Quantitative Confocal Fluorescence Measurements in Living Tissue -- 31 Cytosolic Calcium Measurements with Confocal Microscopy.A variety of powerful techniques to monitor and analyse the events during signal transduction at the single cell level are described in this lab manual. An introductionary section for cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.Life sciences.Pharmacology.Biochemistry.Cell biology.Biomedical engineering.Life Sciences.Cell Biology.Biomedical Engineering.Biochemistry, general.Pharmacology/Toxicology.Springer eBookshttp://dx.doi.org/10.1007/978-3-642-80368-0URN:ISBN:9783642803680

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