Box Wilson factorial design methodology for the optimization of laccase production by Pleurotus djamor in liquid Culture
A surface response design methodology was used to optimize laccase production by Pleurotus djamor. Among six strains ECS-0184 was selected to apply the mathematical approach. ECS-0184 showed a specific growth rate (μ) of 0.87 days-¹ and a laccase yield (YL/X) of 372 UA g-¹ biomass. Predictable optimal conditions obtained were pH 5-6, temperature range 25-28 °C and 3-5 days of cultivation to obtain best laccase yield, 1175 UA g-¹ biomass. Critical factors for laccase production are temperature and day of harvest according with experimental design. The laccase produced by Pleurotus djamor has a molecular weight of 44 kDa; its activity was detected in PAGE gels with ABTS and pyrogallol as substrates.
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| Main Authors: | , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Subjects: | Pleurotus djamor, Cultivo de hongos, Lacasas, |
| Online Access: | http://crmb.aizeonpublishers.net/content/2016/2/no2.html |
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