Effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae)

Ramírez Santos, Edwin Mauricio Doctor 13471; Rendón Arana, Pedro Alfonso Doctor autor/a 21216; Ruiz Montoya, Lorena 1964- Doctora autor/a 5452; Toledo, Jorge Doctor autor/a 5445; Liedo Fernández, Pablo Doctor autor/a 67

Effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae)

The genetically modified strain of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) VIENNA 8 1260, was developed from the genetic sexing strain VIENNA 8. It has two molecular markers that exhibit red fluorescence in the body and green fluorescence in testis and sperm. These traits offer a precise tool to discriminate between mass-reared and wild males, increasing the effectiveness of sterile insect technique. The reproductive performance of the VIENNA 8 1260 and VIENNA 8 D53- (without the D53 inversion introduced to prevent recombination) was compared at different irradiation doses. The general effect of irradiation on VIENNA 8 1260 followed the same patterns documented in previous publications for VIENNA 8 D53-. Irradiation doses of 80 Gray or greater reduced fertility and induced high levels of sterility in wild females. Fecundity reduction was higher in VIENNA 8 1260 than in VIENNA 8 D53- females. Vertical transmission of the fluorescence gene was confirmed up to the F4 generation. Substerilization in the VIENNA 8 1260 could jeopardize the usefulness of the transgenic strain due to the possible vertical transfer of the fluorescence transgene from the sterile males to the wild flies. A biologically safe higher irradiation dose could result in reduced competitiveness of the VIENNA 8 1260 strain. Mating and remating experiments suggest that Mediterranean fruit fly females exhibit a relative precedence in the use of the sperm: though both sperms are mixed, sperm from the remating is spent first. Results suggest a lower fitness of VIENNA 8 1260 sperm, when compared with sperm from a nonfluorescent bisexual strain, which is consistent with the lower reproductive performance documented for the VIENNA 8 1260 strain.

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Bibliographic Details
Main Authors:	Ramírez Santos, Edwin Mauricio Doctor 13471, Rendón Arana, Pedro Alfonso Doctor autor/a 21216, Ruiz Montoya, Lorena 1964- Doctora autor/a 5452, Toledo, Jorge Doctor autor/a 5445, Liedo Fernández, Pablo Doctor autor/a 67
Format:	Texto biblioteca
Language:	eng
Subjects:	Ceratitis capitata, Moscas de la fruta, Técnica del insecto estéril, Fertilidad, Control de plagas, Artfrosur,
Online Access:	https://academic.oup.com/jee/article-lookup/doi/10.1093/jee/tox119
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id	KOHA-OAI-ECOSUR:28635
record_format	koha
institution	ECOSUR
collection	Koha
country	México
countrycode	MX
component	Bibliográfico
access	En linea En linea
databasecode	cat-ecosur
tag	biblioteca
region	America del Norte
libraryname	Sistema de Información Bibliotecario de ECOSUR (SIBE)
language	eng
topic	Ceratitis capitata Moscas de la fruta Técnica del insecto estéril Fertilidad Control de plagas Artfrosur Ceratitis capitata Moscas de la fruta Técnica del insecto estéril Fertilidad Control de plagas Artfrosur
spellingShingle	Ceratitis capitata Moscas de la fruta Técnica del insecto estéril Fertilidad Control de plagas Artfrosur Ceratitis capitata Moscas de la fruta Técnica del insecto estéril Fertilidad Control de plagas Artfrosur Ramírez Santos, Edwin Mauricio Doctor 13471 Rendón Arana, Pedro Alfonso Doctor autor/a 21216 Ruiz Montoya, Lorena 1964- Doctora autor/a 5452 Toledo, Jorge Doctor autor/a 5445 Liedo Fernández, Pablo Doctor autor/a 67 Effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae)
description	The genetically modified strain of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) VIENNA 8 1260, was developed from the genetic sexing strain VIENNA 8. It has two molecular markers that exhibit red fluorescence in the body and green fluorescence in testis and sperm. These traits offer a precise tool to discriminate between mass-reared and wild males, increasing the effectiveness of sterile insect technique. The reproductive performance of the VIENNA 8 1260 and VIENNA 8 D53- (without the D53 inversion introduced to prevent recombination) was compared at different irradiation doses. The general effect of irradiation on VIENNA 8 1260 followed the same patterns documented in previous publications for VIENNA 8 D53-. Irradiation doses of 80 Gray or greater reduced fertility and induced high levels of sterility in wild females. Fecundity reduction was higher in VIENNA 8 1260 than in VIENNA 8 D53- females. Vertical transmission of the fluorescence gene was confirmed up to the F4 generation. Substerilization in the VIENNA 8 1260 could jeopardize the usefulness of the transgenic strain due to the possible vertical transfer of the fluorescence transgene from the sterile males to the wild flies. A biologically safe higher irradiation dose could result in reduced competitiveness of the VIENNA 8 1260 strain. Mating and remating experiments suggest that Mediterranean fruit fly females exhibit a relative precedence in the use of the sperm: though both sperms are mixed, sperm from the remating is spent first. Results suggest a lower fitness of VIENNA 8 1260 sperm, when compared with sperm from a nonfluorescent bisexual strain, which is consistent with the lower reproductive performance documented for the VIENNA 8 1260 strain.
format	Texto
topic_facet	Ceratitis capitata Moscas de la fruta Técnica del insecto estéril Fertilidad Control de plagas Artfrosur
author	Ramírez Santos, Edwin Mauricio Doctor 13471 Rendón Arana, Pedro Alfonso Doctor autor/a 21216 Ruiz Montoya, Lorena 1964- Doctora autor/a 5452 Toledo, Jorge Doctor autor/a 5445 Liedo Fernández, Pablo Doctor autor/a 67
author_facet	Ramírez Santos, Edwin Mauricio Doctor 13471 Rendón Arana, Pedro Alfonso Doctor autor/a 21216 Ruiz Montoya, Lorena 1964- Doctora autor/a 5452 Toledo, Jorge Doctor autor/a 5445 Liedo Fernández, Pablo Doctor autor/a 67
author_sort	Ramírez Santos, Edwin Mauricio Doctor 13471
title	Effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae)
title_short	Effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae)
title_full	Effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae)
title_fullStr	Effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae)
title_full_unstemmed	Effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae)
title_sort	effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (diptera: tephritidae)
url	https://academic.oup.com/jee/article-lookup/doi/10.1093/jee/tox119
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spelling	KOHA-OAI-ECOSUR:286352024-08-26T11:26:56ZEffect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae) Ramírez Santos, Edwin Mauricio Doctor 13471 Rendón Arana, Pedro Alfonso Doctor autor/a 21216 Ruiz Montoya, Lorena 1964- Doctora autor/a 5452 Toledo, Jorge Doctor autor/a 5445 Liedo Fernández, Pablo Doctor autor/a 67 textengThe genetically modified strain of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) VIENNA 8 1260, was developed from the genetic sexing strain VIENNA 8. It has two molecular markers that exhibit red fluorescence in the body and green fluorescence in testis and sperm. These traits offer a precise tool to discriminate between mass-reared and wild males, increasing the effectiveness of sterile insect technique. The reproductive performance of the VIENNA 8 1260 and VIENNA 8 D53- (without the D53 inversion introduced to prevent recombination) was compared at different irradiation doses. The general effect of irradiation on VIENNA 8 1260 followed the same patterns documented in previous publications for VIENNA 8 D53-. Irradiation doses of 80 Gray or greater reduced fertility and induced high levels of sterility in wild females. Fecundity reduction was higher in VIENNA 8 1260 than in VIENNA 8 D53- females. Vertical transmission of the fluorescence gene was confirmed up to the F4 generation. Substerilization in the VIENNA 8 1260 could jeopardize the usefulness of the transgenic strain due to the possible vertical transfer of the fluorescence transgene from the sterile males to the wild flies. A biologically safe higher irradiation dose could result in reduced competitiveness of the VIENNA 8 1260 strain. Mating and remating experiments suggest that Mediterranean fruit fly females exhibit a relative precedence in the use of the sperm: though both sperms are mixed, sperm from the remating is spent first. Results suggest a lower fitness of VIENNA 8 1260 sperm, when compared with sperm from a nonfluorescent bisexual strain, which is consistent with the lower reproductive performance documented for the VIENNA 8 1260 strain.The genetically modified strain of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) VIENNA 8 1260, was developed from the genetic sexing strain VIENNA 8. It has two molecular markers that exhibit red fluorescence in the body and green fluorescence in testis and sperm. These traits offer a precise tool to discriminate between mass-reared and wild males, increasing the effectiveness of sterile insect technique. The reproductive performance of the VIENNA 8 1260 and VIENNA 8 D53- (without the D53 inversion introduced to prevent recombination) was compared at different irradiation doses. The general effect of irradiation on VIENNA 8 1260 followed the same patterns documented in previous publications for VIENNA 8 D53-. Irradiation doses of 80 Gray or greater reduced fertility and induced high levels of sterility in wild females. Fecundity reduction was higher in VIENNA 8 1260 than in VIENNA 8 D53- females. Vertical transmission of the fluorescence gene was confirmed up to the F4 generation. Substerilization in the VIENNA 8 1260 could jeopardize the usefulness of the transgenic strain due to the possible vertical transfer of the fluorescence transgene from the sterile males to the wild flies. A biologically safe higher irradiation dose could result in reduced competitiveness of the VIENNA 8 1260 strain. Mating and remating experiments suggest that Mediterranean fruit fly females exhibit a relative precedence in the use of the sperm: though both sperms are mixed, sperm from the remating is spent first. Results suggest a lower fitness of VIENNA 8 1260 sperm, when compared with sperm from a nonfluorescent bisexual strain, which is consistent with the lower reproductive performance documented for the VIENNA 8 1260 strain.Adobe Acrobat profesional 6.0 o superiorCeratitis capitataMoscas de la frutaTécnica del insecto estérilFertilidadControl de plagasArtfrosurDisponible en líneaJournal of Economic Entomologyhttps://academic.oup.com/jee/article-lookup/doi/10.1093/jee/tox119Disponible para usuarios de ECOSUR con su clave de acceso

Effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae)

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