Consequences of stoichiometric error on nuclear DNA content evaluation in Coffea liberica var. dewevrei using DAPI and propidium iodide
The genome size of coffee trees (Coffea sp.) was assessed using flow cytometry. Nuclear DNA was stained with two dyes (4',6-diamino-2-phenylindole dihydrochloride hydrate (DAPI) and propidium iodide (PI)). Fluorescence in coffee tree nuclei (C-PI orC-DAPI) was compared with that of the standard, petunia (P-PI or P-DAPI). If there is no stoichiometric error, then the ratio between fluorescence of the target nuclei and that of the standard nuclei (R-PI or R-DAPI) is expected to be proportional to thegenome size. Between-tree differences in target:standard fluorescence ratios were noted in Coffea liberica var. dewevrei using propidium iodide and DAPI. For both dyes, between-tree differences were due to a lack of proportionality when comparing locations of the coffee peak and the petunia peak. Intraspecific genome size variations clearly cannot explain variations in the target: standard fluorescence ratio. The origin of the lack of proportionality between target and standard fluorescences differed for the two dyes. With propidium iodide, there was a regression line convergence point, and no between-tree differences were noted in this respect, whereas there was no such convergence with DAPI. An accurate estimate of genome size can thus be obtained with PI. Implications with respect to accessibility and binding mode are discussed.
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | biblioteca |
| Published: |
2002
|
| Subjects: | COFFEA, COFFEA LIBERICA, PETUNIA, HIBRIDOS, CAFE, ADN, GENOMAS, ACIDOS NUCLEICOS, |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
KOHA-OAI-BVE:89172 |
|---|---|
| record_format |
koha |
| spelling |
KOHA-OAI-BVE:891722020-02-03T21:57:02ZConsequences of stoichiometric error on nuclear DNA content evaluation in Coffea liberica var. dewevrei using DAPI and propidium iodide 99614 Noirot, M. 46618 Barre, P. 87745 Louarn, J. 63686 Duperray, C. 75866 Hamon, S. 2002The genome size of coffee trees (Coffea sp.) was assessed using flow cytometry. Nuclear DNA was stained with two dyes (4',6-diamino-2-phenylindole dihydrochloride hydrate (DAPI) and propidium iodide (PI)). Fluorescence in coffee tree nuclei (C-PI orC-DAPI) was compared with that of the standard, petunia (P-PI or P-DAPI). If there is no stoichiometric error, then the ratio between fluorescence of the target nuclei and that of the standard nuclei (R-PI or R-DAPI) is expected to be proportional to thegenome size. Between-tree differences in target:standard fluorescence ratios were noted in Coffea liberica var. dewevrei using propidium iodide and DAPI. For both dyes, between-tree differences were due to a lack of proportionality when comparing locations of the coffee peak and the petunia peak. Intraspecific genome size variations clearly cannot explain variations in the target: standard fluorescence ratio. The origin of the lack of proportionality between target and standard fluorescences differed for the two dyes. With propidium iodide, there was a regression line convergence point, and no between-tree differences were noted in this respect, whereas there was no such convergence with DAPI. An accurate estimate of genome size can thus be obtained with PI. Implications with respect to accessibility and binding mode are discussed.The genome size of coffee trees (Coffea sp.) was assessed using flow cytometry. Nuclear DNA was stained with two dyes (4',6-diamino-2-phenylindole dihydrochloride hydrate (DAPI) and propidium iodide (PI)). Fluorescence in coffee tree nuclei (C-PI orC-DAPI) was compared with that of the standard, petunia (P-PI or P-DAPI). If there is no stoichiometric error, then the ratio between fluorescence of the target nuclei and that of the standard nuclei (R-PI or R-DAPI) is expected to be proportional to thegenome size. Between-tree differences in target:standard fluorescence ratios were noted in Coffea liberica var. dewevrei using propidium iodide and DAPI. For both dyes, between-tree differences were due to a lack of proportionality when comparing locations of the coffee peak and the petunia peak. Intraspecific genome size variations clearly cannot explain variations in the target: standard fluorescence ratio. The origin of the lack of proportionality between target and standard fluorescences differed for the two dyes. With propidium iodide, there was a regression line convergence point, and no between-tree differences were noted in this respect, whereas there was no such convergence with DAPI. An accurate estimate of genome size can thus be obtained with PI. Implications with respect to accessibility and binding mode are discussed.COFFEACOFFEA LIBERICAPETUNIAHIBRIDOSCAFEADNGENOMASACIDOS NUCLEICOSAnnals of Botany (RU) |
| institution |
IICA |
| collection |
Koha |
| country |
Costa Rica |
| countrycode |
CR |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
cat-sibiica |
| tag |
biblioteca |
| region |
America Central |
| libraryname |
Sistema de Bibliotecas IICA/CATIE |
| topic |
COFFEA COFFEA LIBERICA PETUNIA HIBRIDOS CAFE ADN GENOMAS ACIDOS NUCLEICOS COFFEA COFFEA LIBERICA PETUNIA HIBRIDOS CAFE ADN GENOMAS ACIDOS NUCLEICOS |
| spellingShingle |
COFFEA COFFEA LIBERICA PETUNIA HIBRIDOS CAFE ADN GENOMAS ACIDOS NUCLEICOS COFFEA COFFEA LIBERICA PETUNIA HIBRIDOS CAFE ADN GENOMAS ACIDOS NUCLEICOS 99614 Noirot, M. 46618 Barre, P. 87745 Louarn, J. 63686 Duperray, C. 75866 Hamon, S. Consequences of stoichiometric error on nuclear DNA content evaluation in Coffea liberica var. dewevrei using DAPI and propidium iodide |
| description |
The genome size of coffee trees (Coffea sp.) was assessed using flow cytometry. Nuclear DNA was stained with two dyes (4',6-diamino-2-phenylindole dihydrochloride hydrate (DAPI) and propidium iodide (PI)). Fluorescence in coffee tree nuclei (C-PI orC-DAPI) was compared with that of the standard, petunia (P-PI or P-DAPI). If there is no stoichiometric error, then the ratio between fluorescence of the target nuclei and that of the standard nuclei (R-PI or R-DAPI) is expected to be proportional to thegenome size. Between-tree differences in target:standard fluorescence ratios were noted in Coffea liberica var. dewevrei using propidium iodide and DAPI. For both dyes, between-tree differences were due to a lack of proportionality when comparing locations of the coffee peak and the petunia peak. Intraspecific genome size variations clearly cannot explain variations in the target: standard fluorescence ratio. The origin of the lack of proportionality between target and standard fluorescences differed for the two dyes. With propidium iodide, there was a regression line convergence point, and no between-tree differences were noted in this respect, whereas there was no such convergence with DAPI. An accurate estimate of genome size can thus be obtained with PI. Implications with respect to accessibility and binding mode are discussed. |
| format |
|
| topic_facet |
COFFEA COFFEA LIBERICA PETUNIA HIBRIDOS CAFE ADN GENOMAS ACIDOS NUCLEICOS |
| author |
99614 Noirot, M. 46618 Barre, P. 87745 Louarn, J. 63686 Duperray, C. 75866 Hamon, S. |
| author_facet |
99614 Noirot, M. 46618 Barre, P. 87745 Louarn, J. 63686 Duperray, C. 75866 Hamon, S. |
| author_sort |
99614 Noirot, M. |
| title |
Consequences of stoichiometric error on nuclear DNA content evaluation in Coffea liberica var. dewevrei using DAPI and propidium iodide |
| title_short |
Consequences of stoichiometric error on nuclear DNA content evaluation in Coffea liberica var. dewevrei using DAPI and propidium iodide |
| title_full |
Consequences of stoichiometric error on nuclear DNA content evaluation in Coffea liberica var. dewevrei using DAPI and propidium iodide |
| title_fullStr |
Consequences of stoichiometric error on nuclear DNA content evaluation in Coffea liberica var. dewevrei using DAPI and propidium iodide |
| title_full_unstemmed |
Consequences of stoichiometric error on nuclear DNA content evaluation in Coffea liberica var. dewevrei using DAPI and propidium iodide |
| title_sort |
consequences of stoichiometric error on nuclear dna content evaluation in coffea liberica var. dewevrei using dapi and propidium iodide |
| publishDate |
2002 |
| work_keys_str_mv |
AT 99614noirotm consequencesofstoichiometricerroronnucleardnacontentevaluationincoffealibericavardewevreiusingdapiandpropidiumiodide AT 46618barrep consequencesofstoichiometricerroronnucleardnacontentevaluationincoffealibericavardewevreiusingdapiandpropidiumiodide AT 87745louarnj consequencesofstoichiometricerroronnucleardnacontentevaluationincoffealibericavardewevreiusingdapiandpropidiumiodide AT 63686duperrayc consequencesofstoichiometricerroronnucleardnacontentevaluationincoffealibericavardewevreiusingdapiandpropidiumiodide AT 75866hamons consequencesofstoichiometricerroronnucleardnacontentevaluationincoffealibericavardewevreiusingdapiandpropidiumiodide |
| _version_ |
1756059919066333185 |