Transmisión y evaluación del efecto del virus de los anillos cloróticos del café (CoCRSV), sobre el desarrollo de tres variedades de Coffea arabica cultivadas en Colombia
Plant material collected in Andes (Antioquía) and Fusagasugá (Cundinamarca) was used to evaluate the methods of mechanical, graft, insect and seed transmission as well as the effect of Coffee chlorotic ringspot caulimovirus on the growth and development of Caturra, Colombia and Típica varieties. The mechanical transmission efficiency was 60 percent, with an incubation period of 25-35 days in the three coffee varieties and with the presence of mottle, chlorotic spots, interveinal blisters, chlorosis and vein deformation. In the graft transmission it was possible to see chlorotic and etched ringspots, leaf distortion, interveinal chlorosis and rosetting of the buds, with 70 percent transmission efficiency and an incubation period of 60 days in the three varieties. The best transmission efficiency (80 percent) was obtained whit the aphid Toxoptera aurantii, in groups of 10 adults per plant, with an incubation period of 15-20 days in the inoculated plants and with the presence of circular chlorotic or etched lesions, mottle, vein chlorosis, or mosaic. Plants inoculated with the virus throughout the aphid presented growth reduction the after 10 months of evaluation. The inoculated plants development has a reduction in their height of 20, 40 and 60 percent in Típica, Caturra and Colombia varieties. In seed transmission there was no evidence of symptoms. Transmission electron microscopic observations revealed the presence of viroplasts with isometric particles of 50-60 nm in diameter, which are characteristic of the Caulimovirus.
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| Format: | biblioteca |
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| Subjects: | COFFEA ARABICA, VIROSIS, CAULIMOVIRUS, TOXOPTERA AURANTII, TRANSMISION DE ENFERMEDADES, SINTOMAS, COLOMBIA, |
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