Memoria

The effect of germination conditions on the morphology of Coffea arabica L. somatic embryos mass-produced in a one-litre temporary immersion bioreactor (RITA7) was studied with emphasis on direct sowing in soil. Using germinated embryos, direct sowing resulted in a highly successful conversion of embryos into plants. Culture density above 1,600 embryos/11-bioreactor positively affected embryo morphology by causing higher embryonic axis elongation (+4-5mm). At this density, addition of a high sucrose concentration (234 mM), 2 weeks before sowing, promoted an increase in effective plant conversion in soil (78 percent) and a vigorous vegetative growth of the resulting plants. Using this somatic embryogenesis process, 50 000 selected F1 hybrids were recently difunded in America Central.

Bibliographic Details

Main Authors:	104332 Pereira, A., 48644 Bertrand, B., 127776 Vásquez, N., 46930 Barry-Etienne, D., Etienne, Hervé 66009, 120252 Solano, W., 64385 Echeverri, J., 132973 Zamora, L. comps., 10103 IICA, San José (Costa Rica). PROMECAFE, 11655 Instituto del Café de Costa Rica, San José (Costa Rica), 19. Simposio Latinoamericano de Caficultura San José (Costa Rica) 2-6 Oct 2000
Format:	biblioteca
Published:	San José (Costa Rica) 2000
Subjects:	COFFEA ARABICA, EMBRIOGENESIS SOMATICA, CULTIVO IN VITRO, SIEMBRA DIRECTA, GERMINACION,
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