Studies on the use of banana trees (Musa sp.) for coffee and cocoa sheling. 1: Supply of in vitro seedlings, formation and rooting of bud-like body of Musa paradisiaca
An experiment on in-vitro culture of banana had been carried out at the Laboratory of Tissue Culture of the Indonesian Coffee and Cocoa Research Institute. The experiment was aimed to anticipate the need of banana seedlings for temporary shade of young coffee and cocoa, especially for smallholder. It consisted of two experiments. The first experiment was to study the effect of organic N on the formation of bud like body (BLB), while the second one was to study the effect of auxin on root formation of BLB. The first experiment showed that organic N plays an important role on the BLB formation. The best result was achieved by combination of three sources of organic N, i.e. 4 mg/l glutamine, 1 mg/l asparagine and 1 mg/l casein hydrolisate. Each combination of organic N source used in this experiment showed either sinergism or antagonism effect when used simultaneously. The second experiment showed that auxin was effective for the root formation of BLB. The best result was achieved on medium supplemented with 0.3 mg/l NAA.
Main Authors: | , |
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Format: | biblioteca |
Published: |
1993
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Subjects: | COFFEA, THEOBROMA CACAO, SOMBRA, PLANTAS DE SOMBRA, MUSA (PLATANOS), CULTIVO IN VITRO, ENRAIZAMIENTO, AUXINAS, COMPUESTO ORGANICO DEL NITROGENO, |
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Summary: | An experiment on in-vitro culture of banana had been carried out at the Laboratory of Tissue Culture of the Indonesian Coffee and Cocoa Research Institute. The experiment was aimed to anticipate the need of banana seedlings for temporary shade of young coffee and cocoa, especially for smallholder. It consisted of two experiments. The first experiment was to study the effect of organic N on the formation of bud like body (BLB), while the second one was to study the effect of auxin on root formation of BLB. The first experiment showed that organic N plays an important role on the BLB formation. The best result was achieved by combination of three sources of organic N, i.e. 4 mg/l glutamine, 1 mg/l asparagine and 1 mg/l casein hydrolisate. Each combination of organic N source used in this experiment showed either sinergism or antagonism effect when used simultaneously. The second experiment showed that auxin was effective for the root formation of BLB. The best result was achieved on medium supplemented with 0.3 mg/l NAA. |
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