Practical approaches for knock - out gene editing in pigs

Practical approaches for knock - out gene editing in pigs

Pigs are an important resource for meat production and serve as a model for human diseases. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for biomedical research. Although, in the past pig have not been widely used partially because of the difficulty in genetic modification; nowadays, with the new revolutionary technology of programmable nucleases, and fundamentally of the CRISPR-Cas9 systems, it is possible for the first time to precisely modify the porcine genome as never before. To this purpose, it is necessary to introduce the system into early stage zygotes or to edit cells followed by somatic cell nuclear transfer. In this review, several strategies for pig knock-out gene editing, using the CRISPR-Cas9 system, will be summarized, as well as genotyping methods and different delivery techniques to introduce these tools into the embryos. Finally, the best approaches to produce homogeneous, biallelic edited animals will be discussed.

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Main Authors: Ratner, Laura Daniela, La Motta, Gaston Emilio, Briski, Olinda, Salamone, Daniel Felipe, Fernández Martín, Rafael
Format: Texto biblioteca
Language:eng
Subjects:CRISPR CAS9, KNOCK OUT, ELECTROPORATION, MICROINJECTION, PORCINE ZYGOTES, SCNT, ,
Online Access:http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=54737
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spelling KOHA-OAI-AGRO:547372024-12-13T11:23:40Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=54737http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGPractical approaches for knock - out gene editing in pigsRatner, Laura DanielaLa Motta, Gaston EmilioBriski, OlindaSalamone, Daniel FelipeFernández Martín, Rafaeltextengapplication/pdfPigs are an important resource for meat production and serve as a model for human diseases. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for biomedical research. Although, in the past pig have not been widely used partially because of the difficulty in genetic modification; nowadays, with the new revolutionary technology of programmable nucleases, and fundamentally of the CRISPR-Cas9 systems, it is possible for the first time to precisely modify the porcine genome as never before. To this purpose, it is necessary to introduce the system into early stage zygotes or to edit cells followed by somatic cell nuclear transfer. In this review, several strategies for pig knock-out gene editing, using the CRISPR-Cas9 system, will be summarized, as well as genotyping methods and different delivery techniques to introduce these tools into the embryos. Finally, the best approaches to produce homogeneous, biallelic edited animals will be discussed.Pigs are an important resource for meat production and serve as a model for human diseases. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for biomedical research. Although, in the past pig have not been widely used partially because of the difficulty in genetic modification; nowadays, with the new revolutionary technology of programmable nucleases, and fundamentally of the CRISPR-Cas9 systems, it is possible for the first time to precisely modify the porcine genome as never before. To this purpose, it is necessary to introduce the system into early stage zygotes or to edit cells followed by somatic cell nuclear transfer. In this review, several strategies for pig knock-out gene editing, using the CRISPR-Cas9 system, will be summarized, as well as genotyping methods and different delivery techniques to introduce these tools into the embryos. Finally, the best approaches to produce homogeneous, biallelic edited animals will be discussed.CRISPR CAS9KNOCK OUTELECTROPORATIONMICROINJECTIONPORCINE ZYGOTESSCNTFrontiers in Genetics
institution UBA FA
collection Koha
country Argentina
countrycode AR
component Bibliográfico
access En linea
En linea
databasecode cat-ceiba
tag biblioteca
region America del Sur
libraryname Biblioteca Central FAUBA
language eng
topic CRISPR CAS9
KNOCK OUT
ELECTROPORATION
MICROINJECTION
PORCINE ZYGOTES
SCNT

CRISPR CAS9
KNOCK OUT
ELECTROPORATION
MICROINJECTION
PORCINE ZYGOTES
SCNT
spellingShingle CRISPR CAS9
KNOCK OUT
ELECTROPORATION
MICROINJECTION
PORCINE ZYGOTES
SCNT

CRISPR CAS9
KNOCK OUT
ELECTROPORATION
MICROINJECTION
PORCINE ZYGOTES
SCNT
Ratner, Laura Daniela
La Motta, Gaston Emilio
Briski, Olinda
Salamone, Daniel Felipe
Fernández Martín, Rafael
Practical approaches for knock - out gene editing in pigs
description Pigs are an important resource for meat production and serve as a model for human diseases. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for biomedical research. Although, in the past pig have not been widely used partially because of the difficulty in genetic modification; nowadays, with the new revolutionary technology of programmable nucleases, and fundamentally of the CRISPR-Cas9 systems, it is possible for the first time to precisely modify the porcine genome as never before. To this purpose, it is necessary to introduce the system into early stage zygotes or to edit cells followed by somatic cell nuclear transfer. In this review, several strategies for pig knock-out gene editing, using the CRISPR-Cas9 system, will be summarized, as well as genotyping methods and different delivery techniques to introduce these tools into the embryos. Finally, the best approaches to produce homogeneous, biallelic edited animals will be discussed.
format Texto
topic_facet
CRISPR CAS9
KNOCK OUT
ELECTROPORATION
MICROINJECTION
PORCINE ZYGOTES
SCNT
author Ratner, Laura Daniela
La Motta, Gaston Emilio
Briski, Olinda
Salamone, Daniel Felipe
Fernández Martín, Rafael
author_facet Ratner, Laura Daniela
La Motta, Gaston Emilio
Briski, Olinda
Salamone, Daniel Felipe
Fernández Martín, Rafael
author_sort Ratner, Laura Daniela
title Practical approaches for knock - out gene editing in pigs
title_short Practical approaches for knock - out gene editing in pigs
title_full Practical approaches for knock - out gene editing in pigs
title_fullStr Practical approaches for knock - out gene editing in pigs
title_full_unstemmed Practical approaches for knock - out gene editing in pigs
title_sort practical approaches for knock - out gene editing in pigs
url http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=54737
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
work_keys_str_mv AT ratnerlauradaniela practicalapproachesforknockoutgeneeditinginpigs
AT lamottagastonemilio practicalapproachesforknockoutgeneeditinginpigs
AT briskiolinda practicalapproachesforknockoutgeneeditinginpigs
AT salamonedanielfelipe practicalapproachesforknockoutgeneeditinginpigs
AT fernandezmartinrafael practicalapproachesforknockoutgeneeditinginpigs
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