Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis
While studying blue light-independent effects of cryptochrome 1 [cry1] photoreceptor, we observed premature opening of the hook in cry1 mutants grown in complete darkness, a phenotype that resembles the one described for the heterotrimeric G-protein alpha subunit [GPA1] null mutant gpa1. Both cry1 and gpa1 also showed reduced accumulation of anthocyanin under blue light. These convergent gpa1 and cry1 phenotypes required the presence of sucrose in the growth media and were not additive in the cry1gpa1 double mutant, suggesting context-dependent signaling convergence between cry1 and GPA1 signaling pathways. Both, gpa1 and cry1 mutants showed reduced GTP-binding activity. The cry1 mutant showed wild-type levels of GPA1 mRNA or GPA1 protein. However, an anti-transducin antibody [AS/7] typically used for plant G alpha proteins, recognized a 54 kDa band in the wild type but not in gpa1 and cry1 mutants. We propose a model where cry1-mediated post-translational modification of GPA1 alters its GTP-binding activity.
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| Subjects: | ARABIDOPSIS THALIANA, CRY1, GPA1, HETEROTRIMERIC G-PROTEINS, SIGNAL TRANSDUCTION, ANTHOCYANIN, ARABIDOPSIS PROTEIN, CRY1 PROTEIN, ARABIDOPSIS, CRYPTOCHROME, GPA1 PROTEIN, ARABIDOPSIS, GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT, MESSENGER RNA, PLANT RNA, SUCROSE, ARABIDOPSIS, BIOLOGICAL MODEL, BIOSYNTHESIS, DRUG EFFECT, GENE EXPRESSION REGULATION, GENETICS, LIGHT, METABOLISM, MUTATION, PHENOTYPE, PHYSIOLOGY, PLANT GROWTH, PROTEIN BINDING, PROTEIN PROCESSING, RADIATION EXPOSURE, SEEDLING, ANTHOCYANINS, ARABIDOPSIS PROTEINS, CRYPTOCHROMES, GENE EXPRESSION REGULATION, PLANT, GTP-BINDING PROTEIN ALPHA SUBUNITS, HYPOCOTYL, MODELS, BIOLOGICAL, PROTEIN PROCESSING, POST-TRANSLATIONAL, RNA, MESSENGER, RNA, PLANT, |
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KOHA-OAI-AGRO:467552022-10-26T11:23:36Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=46755AAGCry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in ArabidopsisFox, Ana RominaSoto, Gabriela Cynthia Jones, Alan M.Casal, Jorge JoséMuschietti, Jorge PrometeoMazzella, María Agustinatextengapplication/pdfWhile studying blue light-independent effects of cryptochrome 1 [cry1] photoreceptor, we observed premature opening of the hook in cry1 mutants grown in complete darkness, a phenotype that resembles the one described for the heterotrimeric G-protein alpha subunit [GPA1] null mutant gpa1. Both cry1 and gpa1 also showed reduced accumulation of anthocyanin under blue light. These convergent gpa1 and cry1 phenotypes required the presence of sucrose in the growth media and were not additive in the cry1gpa1 double mutant, suggesting context-dependent signaling convergence between cry1 and GPA1 signaling pathways. Both, gpa1 and cry1 mutants showed reduced GTP-binding activity. The cry1 mutant showed wild-type levels of GPA1 mRNA or GPA1 protein. However, an anti-transducin antibody [AS/7] typically used for plant G alpha proteins, recognized a 54 kDa band in the wild type but not in gpa1 and cry1 mutants. We propose a model where cry1-mediated post-translational modification of GPA1 alters its GTP-binding activity.While studying blue light-independent effects of cryptochrome 1 [cry1] photoreceptor, we observed premature opening of the hook in cry1 mutants grown in complete darkness, a phenotype that resembles the one described for the heterotrimeric G-protein alpha subunit [GPA1] null mutant gpa1. Both cry1 and gpa1 also showed reduced accumulation of anthocyanin under blue light. These convergent gpa1 and cry1 phenotypes required the presence of sucrose in the growth media and were not additive in the cry1gpa1 double mutant, suggesting context-dependent signaling convergence between cry1 and GPA1 signaling pathways. Both, gpa1 and cry1 mutants showed reduced GTP-binding activity. The cry1 mutant showed wild-type levels of GPA1 mRNA or GPA1 protein. However, an anti-transducin antibody [AS/7] typically used for plant G alpha proteins, recognized a 54 kDa band in the wild type but not in gpa1 and cry1 mutants. We propose a model where cry1-mediated post-translational modification of GPA1 alters its GTP-binding activity.ARABIDOPSIS THALIANACRY1GPA1HETEROTRIMERIC G-PROTEINSSIGNAL TRANSDUCTIONANTHOCYANINARABIDOPSIS PROTEINCRY1 PROTEIN, ARABIDOPSISCRYPTOCHROMEGPA1 PROTEIN, ARABIDOPSISGUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNITMESSENGER RNAPLANT RNASUCROSEARABIDOPSISBIOLOGICAL MODELBIOSYNTHESISDRUG EFFECTGENE EXPRESSION REGULATIONGENETICSLIGHTMETABOLISMMUTATIONPHENOTYPEPHYSIOLOGYPLANT GROWTHPROTEIN BINDINGPROTEIN PROCESSINGRADIATION EXPOSURESEEDLINGANTHOCYANINSARABIDOPSIS PROTEINSCRYPTOCHROMESGENE EXPRESSION REGULATION, PLANTGTP-BINDING PROTEIN ALPHA SUBUNITSHYPOCOTYLMODELS, BIOLOGICALPROTEIN PROCESSING, POST-TRANSLATIONALRNA, MESSENGERRNA, PLANTPlant Molecular Biology |
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ARABIDOPSIS THALIANA CRY1 GPA1 HETEROTRIMERIC G-PROTEINS SIGNAL TRANSDUCTION ANTHOCYANIN ARABIDOPSIS PROTEIN CRY1 PROTEIN, ARABIDOPSIS CRYPTOCHROME GPA1 PROTEIN, ARABIDOPSIS GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT MESSENGER RNA PLANT RNA SUCROSE ARABIDOPSIS BIOLOGICAL MODEL BIOSYNTHESIS DRUG EFFECT GENE EXPRESSION REGULATION GENETICS LIGHT METABOLISM MUTATION PHENOTYPE PHYSIOLOGY PLANT GROWTH PROTEIN BINDING PROTEIN PROCESSING RADIATION EXPOSURE SEEDLING ANTHOCYANINS ARABIDOPSIS PROTEINS CRYPTOCHROMES GENE EXPRESSION REGULATION, PLANT GTP-BINDING PROTEIN ALPHA SUBUNITS HYPOCOTYL MODELS, BIOLOGICAL PROTEIN PROCESSING, POST-TRANSLATIONAL RNA, MESSENGER RNA, PLANT ARABIDOPSIS THALIANA CRY1 GPA1 HETEROTRIMERIC G-PROTEINS SIGNAL TRANSDUCTION ANTHOCYANIN ARABIDOPSIS PROTEIN CRY1 PROTEIN, ARABIDOPSIS CRYPTOCHROME GPA1 PROTEIN, ARABIDOPSIS GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT MESSENGER RNA PLANT RNA SUCROSE ARABIDOPSIS BIOLOGICAL MODEL BIOSYNTHESIS DRUG EFFECT GENE EXPRESSION REGULATION GENETICS LIGHT METABOLISM MUTATION PHENOTYPE PHYSIOLOGY PLANT GROWTH PROTEIN BINDING PROTEIN PROCESSING RADIATION EXPOSURE SEEDLING ANTHOCYANINS ARABIDOPSIS PROTEINS CRYPTOCHROMES GENE EXPRESSION REGULATION, PLANT GTP-BINDING PROTEIN ALPHA SUBUNITS HYPOCOTYL MODELS, BIOLOGICAL PROTEIN PROCESSING, POST-TRANSLATIONAL RNA, MESSENGER RNA, PLANT |
| spellingShingle |
ARABIDOPSIS THALIANA CRY1 GPA1 HETEROTRIMERIC G-PROTEINS SIGNAL TRANSDUCTION ANTHOCYANIN ARABIDOPSIS PROTEIN CRY1 PROTEIN, ARABIDOPSIS CRYPTOCHROME GPA1 PROTEIN, ARABIDOPSIS GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT MESSENGER RNA PLANT RNA SUCROSE ARABIDOPSIS BIOLOGICAL MODEL BIOSYNTHESIS DRUG EFFECT GENE EXPRESSION REGULATION GENETICS LIGHT METABOLISM MUTATION PHENOTYPE PHYSIOLOGY PLANT GROWTH PROTEIN BINDING PROTEIN PROCESSING RADIATION EXPOSURE SEEDLING ANTHOCYANINS ARABIDOPSIS PROTEINS CRYPTOCHROMES GENE EXPRESSION REGULATION, PLANT GTP-BINDING PROTEIN ALPHA SUBUNITS HYPOCOTYL MODELS, BIOLOGICAL PROTEIN PROCESSING, POST-TRANSLATIONAL RNA, MESSENGER RNA, PLANT ARABIDOPSIS THALIANA CRY1 GPA1 HETEROTRIMERIC G-PROTEINS SIGNAL TRANSDUCTION ANTHOCYANIN ARABIDOPSIS PROTEIN CRY1 PROTEIN, ARABIDOPSIS CRYPTOCHROME GPA1 PROTEIN, ARABIDOPSIS GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT MESSENGER RNA PLANT RNA SUCROSE ARABIDOPSIS BIOLOGICAL MODEL BIOSYNTHESIS DRUG EFFECT GENE EXPRESSION REGULATION GENETICS LIGHT METABOLISM MUTATION PHENOTYPE PHYSIOLOGY PLANT GROWTH PROTEIN BINDING PROTEIN PROCESSING RADIATION EXPOSURE SEEDLING ANTHOCYANINS ARABIDOPSIS PROTEINS CRYPTOCHROMES GENE EXPRESSION REGULATION, PLANT GTP-BINDING PROTEIN ALPHA SUBUNITS HYPOCOTYL MODELS, BIOLOGICAL PROTEIN PROCESSING, POST-TRANSLATIONAL RNA, MESSENGER RNA, PLANT Fox, Ana Romina Soto, Gabriela Cynthia Jones, Alan M. Casal, Jorge José Muschietti, Jorge Prometeo Mazzella, María Agustina Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis |
| description |
While studying blue light-independent effects of cryptochrome 1 [cry1] photoreceptor, we observed premature opening of the hook in cry1 mutants grown in complete darkness, a phenotype that resembles the one described for the heterotrimeric G-protein alpha subunit [GPA1] null mutant gpa1. Both cry1 and gpa1 also showed reduced accumulation of anthocyanin under blue light. These convergent gpa1 and cry1 phenotypes required the presence of sucrose in the growth media and were not additive in the cry1gpa1 double mutant, suggesting context-dependent signaling convergence between cry1 and GPA1 signaling pathways. Both, gpa1 and cry1 mutants showed reduced GTP-binding activity. The cry1 mutant showed wild-type levels of GPA1 mRNA or GPA1 protein. However, an anti-transducin antibody [AS/7] typically used for plant G alpha proteins, recognized a 54 kDa band in the wild type but not in gpa1 and cry1 mutants. We propose a model where cry1-mediated post-translational modification of GPA1 alters its GTP-binding activity. |
| format |
Texto |
| topic_facet |
ARABIDOPSIS THALIANA CRY1 GPA1 HETEROTRIMERIC G-PROTEINS SIGNAL TRANSDUCTION ANTHOCYANIN ARABIDOPSIS PROTEIN CRY1 PROTEIN, ARABIDOPSIS CRYPTOCHROME GPA1 PROTEIN, ARABIDOPSIS GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT MESSENGER RNA PLANT RNA SUCROSE ARABIDOPSIS BIOLOGICAL MODEL BIOSYNTHESIS DRUG EFFECT GENE EXPRESSION REGULATION GENETICS LIGHT METABOLISM MUTATION PHENOTYPE PHYSIOLOGY PLANT GROWTH PROTEIN BINDING PROTEIN PROCESSING RADIATION EXPOSURE SEEDLING ANTHOCYANINS ARABIDOPSIS PROTEINS CRYPTOCHROMES GENE EXPRESSION REGULATION, PLANT GTP-BINDING PROTEIN ALPHA SUBUNITS HYPOCOTYL MODELS, BIOLOGICAL PROTEIN PROCESSING, POST-TRANSLATIONAL RNA, MESSENGER RNA, PLANT |
| author |
Fox, Ana Romina Soto, Gabriela Cynthia Jones, Alan M. Casal, Jorge José Muschietti, Jorge Prometeo Mazzella, María Agustina |
| author_facet |
Fox, Ana Romina Soto, Gabriela Cynthia Jones, Alan M. Casal, Jorge José Muschietti, Jorge Prometeo Mazzella, María Agustina |
| author_sort |
Fox, Ana Romina |
| title |
Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis |
| title_short |
Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis |
| title_full |
Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis |
| title_fullStr |
Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis |
| title_full_unstemmed |
Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis |
| title_sort |
cry1 and gpa1 signaling genetically interact in hook opening and anthocyanin synthesis in arabidopsis |
| url |
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=46755 |
| work_keys_str_mv |
AT foxanaromina cry1andgpa1signalinggeneticallyinteractinhookopeningandanthocyaninsynthesisinarabidopsis AT sotogabrielacynthia cry1andgpa1signalinggeneticallyinteractinhookopeningandanthocyaninsynthesisinarabidopsis AT jonesalanm cry1andgpa1signalinggeneticallyinteractinhookopeningandanthocyaninsynthesisinarabidopsis AT casaljorgejose cry1andgpa1signalinggeneticallyinteractinhookopeningandanthocyaninsynthesisinarabidopsis AT muschiettijorgeprometeo cry1andgpa1signalinggeneticallyinteractinhookopeningandanthocyaninsynthesisinarabidopsis AT mazzellamariaagustina cry1andgpa1signalinggeneticallyinteractinhookopeningandanthocyaninsynthesisinarabidopsis |
| _version_ |
1756046686563598336 |