Characterization of the two component regulatory system PhoPR in Mycobacterium bovis

Characterization of the two component regulatory system PhoPR in Mycobacterium bovis

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Mycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown that the genes encoding the two - component system PhoPR, which regulates several genes involved in the virulence of M. tuberculosis, are polymorphic in M. bovis, when compared to M. tuberculosis, which results in a dysfunctional two - component system. In this study we investigated the role of PhoPR in two M. bovis strains with differing degrees of virulence. We found that the deletion of phoP in an M. bovis isolate reduced its capacity of inducing phagosomal arrest in bovine macrophages. By gene expression analysis, we demonstrated that, in both M. bovis strains, PhoP regulates the expression of a putative lipid desaturase Mb1404-Mb1405, a protein involved in redox stress AhpC, the sulfolipid transporter Mmpl8 and the secreted antigen ESAT-6. Furthermore, the lack of PhoP increased the sensitivity to acidic stress and alteration of the biofilm/pellicle formation of M. bovis. Both these phenotypes are connected to bacterial redox homeostasis. Therefore, the results of this study suggest a role of PhoPR in M. bovis to be linked to the mechanisms that mycobacteria display to maintain their redox balance.

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Main Authors: García, Elizabeth A., Blanco, Federico Carlos, Bigi, María Mercedes, Vázquez, Cristina Lourdes, Forrellad, Marina Andrea, Rocha, Rosana Valeria, Golby, Paul, Soria, Marcelo Abel, Bigi, Fabiana
Format: Texto biblioteca
Language:eng
Subjects:MYCOBACTERIUM BOVIS, PHOPR, TWO - COMPONENT SYSTEM, GENE EXPRESSION,
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spelling KOHA-OAI-AGRO:459522023-11-23T14:52:03Zhttp://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=45952http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=AAGCharacterization of the two component regulatory system PhoPR in Mycobacterium bovisGarcía, Elizabeth A.Blanco, Federico CarlosBigi, María MercedesVázquez, Cristina LourdesForrellad, Marina AndreaRocha, Rosana ValeriaGolby, PaulSoria, Marcelo AbelBigi, Fabianatextengapplication/pdfMycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown that the genes encoding the two - component system PhoPR, which regulates several genes involved in the virulence of M. tuberculosis, are polymorphic in M. bovis, when compared to M. tuberculosis, which results in a dysfunctional two - component system. In this study we investigated the role of PhoPR in two M. bovis strains with differing degrees of virulence. We found that the deletion of phoP in an M. bovis isolate reduced its capacity of inducing phagosomal arrest in bovine macrophages. By gene expression analysis, we demonstrated that, in both M. bovis strains, PhoP regulates the expression of a putative lipid desaturase Mb1404-Mb1405, a protein involved in redox stress AhpC, the sulfolipid transporter Mmpl8 and the secreted antigen ESAT-6. Furthermore, the lack of PhoP increased the sensitivity to acidic stress and alteration of the biofilm/pellicle formation of M. bovis. Both these phenotypes are connected to bacterial redox homeostasis. Therefore, the results of this study suggest a role of PhoPR in M. bovis to be linked to the mechanisms that mycobacteria display to maintain their redox balance.Mycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown that the genes encoding the two - component system PhoPR, which regulates several genes involved in the virulence of M. tuberculosis, are polymorphic in M. bovis, when compared to M. tuberculosis, which results in a dysfunctional two - component system. In this study we investigated the role of PhoPR in two M. bovis strains with differing degrees of virulence. We found that the deletion of phoP in an M. bovis isolate reduced its capacity of inducing phagosomal arrest in bovine macrophages. By gene expression analysis, we demonstrated that, in both M. bovis strains, PhoP regulates the expression of a putative lipid desaturase Mb1404-Mb1405, a protein involved in redox stress AhpC, the sulfolipid transporter Mmpl8 and the secreted antigen ESAT-6. Furthermore, the lack of PhoP increased the sensitivity to acidic stress and alteration of the biofilm/pellicle formation of M. bovis. Both these phenotypes are connected to bacterial redox homeostasis. Therefore, the results of this study suggest a role of PhoPR in M. bovis to be linked to the mechanisms that mycobacteria display to maintain their redox balance.MYCOBACTERIUM BOVISPHOPRTWO - COMPONENT SYSTEMGENE EXPRESSIONVeterinary Microbiology
institution UBA FA
collection Koha
country Argentina
countrycode AR
component Bibliográfico
access En linea
En linea
databasecode cat-ceiba
tag biblioteca
region America del Sur
libraryname Biblioteca Central FAUBA
language eng
topic MYCOBACTERIUM BOVIS
PHOPR
TWO - COMPONENT SYSTEM
GENE EXPRESSION
MYCOBACTERIUM BOVIS
PHOPR
TWO - COMPONENT SYSTEM
GENE EXPRESSION
spellingShingle MYCOBACTERIUM BOVIS
PHOPR
TWO - COMPONENT SYSTEM
GENE EXPRESSION
MYCOBACTERIUM BOVIS
PHOPR
TWO - COMPONENT SYSTEM
GENE EXPRESSION
García, Elizabeth A.
Blanco, Federico Carlos
Bigi, María Mercedes
Vázquez, Cristina Lourdes
Forrellad, Marina Andrea
Rocha, Rosana Valeria
Golby, Paul
Soria, Marcelo Abel
Bigi, Fabiana
Characterization of the two component regulatory system PhoPR in Mycobacterium bovis
description Mycobacterium bovis is the causative agent of bovine tuberculosis and is a member of Mycobacterium tuberculosis complex, which causes tuberculosis in a number of mammals including humans. Previous studies have shown that the genes encoding the two - component system PhoPR, which regulates several genes involved in the virulence of M. tuberculosis, are polymorphic in M. bovis, when compared to M. tuberculosis, which results in a dysfunctional two - component system. In this study we investigated the role of PhoPR in two M. bovis strains with differing degrees of virulence. We found that the deletion of phoP in an M. bovis isolate reduced its capacity of inducing phagosomal arrest in bovine macrophages. By gene expression analysis, we demonstrated that, in both M. bovis strains, PhoP regulates the expression of a putative lipid desaturase Mb1404-Mb1405, a protein involved in redox stress AhpC, the sulfolipid transporter Mmpl8 and the secreted antigen ESAT-6. Furthermore, the lack of PhoP increased the sensitivity to acidic stress and alteration of the biofilm/pellicle formation of M. bovis. Both these phenotypes are connected to bacterial redox homeostasis. Therefore, the results of this study suggest a role of PhoPR in M. bovis to be linked to the mechanisms that mycobacteria display to maintain their redox balance.
format Texto
topic_facet MYCOBACTERIUM BOVIS
PHOPR
TWO - COMPONENT SYSTEM
GENE EXPRESSION
author García, Elizabeth A.
Blanco, Federico Carlos
Bigi, María Mercedes
Vázquez, Cristina Lourdes
Forrellad, Marina Andrea
Rocha, Rosana Valeria
Golby, Paul
Soria, Marcelo Abel
Bigi, Fabiana
author_facet García, Elizabeth A.
Blanco, Federico Carlos
Bigi, María Mercedes
Vázquez, Cristina Lourdes
Forrellad, Marina Andrea
Rocha, Rosana Valeria
Golby, Paul
Soria, Marcelo Abel
Bigi, Fabiana
author_sort García, Elizabeth A.
title Characterization of the two component regulatory system PhoPR in Mycobacterium bovis
title_short Characterization of the two component regulatory system PhoPR in Mycobacterium bovis
title_full Characterization of the two component regulatory system PhoPR in Mycobacterium bovis
title_fullStr Characterization of the two component regulatory system PhoPR in Mycobacterium bovis
title_full_unstemmed Characterization of the two component regulatory system PhoPR in Mycobacterium bovis
title_sort characterization of the two component regulatory system phopr in mycobacterium bovis
url http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=45952
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
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http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=
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