Molecular identification of the secondary endosymbiont Hamiltonella defensa in the rose-grain aphid Metopolophium dirhodum

This is the first report of the association between the rose-grain aphid Metopolophium dirhodum, a potentially important cereal pest and the facultative symbiont Hamiltonella defensa. The infection with this gamma-proteobacterium was determined by PCR in laboratory-reared and field-collected specimens of an Argentinian population of the aphid. Partial bacterial 16S, IGS and 23S rRNA genes were sequenced and compared to other available Hamiltonella sequences by phylogenetic analysis. The present study provides new information on previously unknown M. dirhodum microbiota.

Bibliographic Details

Main Authors:	Telesnicki, M. C., Ghersa, C. M., Martínez-Ghersa, M. A., Arneodo, J. D.
Format:	article biblioteca
Language:	eng
Subjects:	APHIDIDAE, FACULTATIVE SYMBIONT, GAMMA-PROTEOBACTERIA, PCR, SEQUENCING, INTERNAL TRANSCRIBED SPACER, RNA 16S, RNA 23S, APHID, BACTERIUM IDENTIFICATION, GAMMAPROTEOBACTERIA, GENE SEQUENCE, GEOGRAPHIC DISTRIBUTION, HAMILTONELLA DEFENSA, HUMAN, INFECTION RATE, METOPOLOPHIUM DIRHODUM, NUCLEOTIDE SEQUENCE, PHYLOGENETIC TREE, PHYLOGENY, POLYMERASE CHAIN REACTION, ANIMALS, APHIDS, PROTEOBACTERIA, SEQUENCE ANALYSIS, RNA, SYMBIOSIS,
Online Access:	http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012Telesnicki
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