Coenurosis in cattle in Uruguay
Coenurosis is a disease caused by Coenurus cerebralis, the larval stage of Taenia multiceps. Ruminants, equids, swine and even humans can be affected developing neurological symptoms. This work reports C. cerebralis infection diagnosed by macroscopic and histologic lesions and molecular tools (PCR/ sequencing) in two heifers in Uruguay. Two 2-3 years old Hereford heifers were necropsied (case A and B). Both animals presented progressive deterioration, ataxia, circling and head deviation. Grossly, in case A, a cystic vesicle protruding from the left hemisphere was observed. The vesicle was broken spontaneously while the encephalon was retrieved from the cranial cavity. On case B, the whole cystic vesicle was retrieved from the right hemisphere. Through the translucent wall´s vesicle was possible to visualize whitish scolices attached to the inner layer. Histopathology of the central nervous system revealed an inflammatory reaction on the cyst adjacent tissue, characterized by the presence of macrophages, eosinophils, lymphocytes and giant cells. The vesicle of case B was dissected and a small piece was used for DNA extraction. By PCR, a fragment of nad1 gene was amplified and sent for direct sequencing. The obtained sequence was compared with the available sequences registered on GenBank using the BLAST tool. The result revealed a 99% homology with sequences registered as T. multiceps. Therefore, this work confirmed that bovine coenurosis is present in Uruguay.
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Sociedad de Medicina Veterinaria del Uruguay (SMVU)
2017
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rev-smvu-uy-article-992019-09-07T22:22:49Z Coenurosis in cattle in Uruguay Coenurosis bovina en Uruguay Buroni, F. Armua-Fernandez, T. M. Nan, F. Matto, C. Venzal, J. M. Rivero, R. Coenurosis bovina Coenurus cerebralis Bos taurus Uruguay Bovine coenurosis Coenurus cerebralis Bos taurus Uruguay Coenurosis is a disease caused by Coenurus cerebralis, the larval stage of Taenia multiceps. Ruminants, equids, swine and even humans can be affected developing neurological symptoms. This work reports C. cerebralis infection diagnosed by macroscopic and histologic lesions and molecular tools (PCR/ sequencing) in two heifers in Uruguay. Two 2-3 years old Hereford heifers were necropsied (case A and B). Both animals presented progressive deterioration, ataxia, circling and head deviation. Grossly, in case A, a cystic vesicle protruding from the left hemisphere was observed. The vesicle was broken spontaneously while the encephalon was retrieved from the cranial cavity. On case B, the whole cystic vesicle was retrieved from the right hemisphere. Through the translucent wall´s vesicle was possible to visualize whitish scolices attached to the inner layer. Histopathology of the central nervous system revealed an inflammatory reaction on the cyst adjacent tissue, characterized by the presence of macrophages, eosinophils, lymphocytes and giant cells. The vesicle of case B was dissected and a small piece was used for DNA extraction. By PCR, a fragment of nad1 gene was amplified and sent for direct sequencing. The obtained sequence was compared with the available sequences registered on GenBank using the BLAST tool. The result revealed a 99% homology with sequences registered as T. multiceps. Therefore, this work confirmed that bovine coenurosis is present in Uruguay. Coenurosis es la enfermedad causada por Coenurus cerebralis, forma larvaria de Taenia multiceps. Afecta a rumiantes, equinos, suinos y humanos provocando sintomatología nerviosa. El presente trabajo tuvo como objetivo describir un foco de coenurosis en bovinos en Uruguay, diagnosticado mediante estudios anatomopatológicos y técnicas moleculares (PCR/secuenciación). Se realizó la necropsia de dos vaquillonas Hereford (Caso A y B) de 2-3 años que presentaban desmejoramiento progresivo, ataxia, torneo y desviación de la cabeza. Macroscópicamente, se observó la presencia de una vesícula quística en el hemisferio cerebral izquierdo del caso A, la cual se rompió espontáneamente al extraer el encéfalo del cráneo. En el caso B, se extrajo del hemisferio cerebral derecho una vesícula completa, de color transparente con escólices blanquecinos en su interior. Al examen histopatológico del sistema nervioso central se observó reacción inflamatoria en el tejido adyacente a la ubicación de los quistes constituida por macrófagos, eosinófilos, linfocitos y células gigantes. La vesícula del caso B fue diseccionada y se utilizó un trozo para la extracción de ADN. Mediante PCR se amplificó un fragmento del gen nad1. La secuencia obtenida fue comparada con las secuencias registradas en el GenBank utilizando la herramienta BLAST. El resultado reveló una homología de 99% con T. multiceps. Por lo tanto, este trabajo confirma que la coenurosis bovina se encuentra presente Uruguay. Sociedad de Medicina Veterinaria del Uruguay (SMVU) 2017-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed article. Artículo evaluado por pares. Artigo revisado por pares. application/pdf https://www.revistasmvu.com.uy/index.php/smvu/article/view/99 Veterinaria (Montevideo); Vol. 53 No. 206 (2017): June; 19-24 Veterinaria (Montevideo); Vol. 53 Núm. 206 (2017): Junio; 19-24 Veterinaria (Montevideo); v. 53 n. 206 (2017): Junho; 19-24 1688-4809 0376-4362 spa https://www.revistasmvu.com.uy/index.php/smvu/article/view/99/45 Derechos de autor 2017 Sociedad de Medicina Veterinaria del Uruguay |
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Buroni, F. Armua-Fernandez, T. M. Nan, F. Matto, C. Venzal, J. M. Rivero, R. |
| spellingShingle |
Buroni, F. Armua-Fernandez, T. M. Nan, F. Matto, C. Venzal, J. M. Rivero, R. Coenurosis in cattle in Uruguay |
| author_facet |
Buroni, F. Armua-Fernandez, T. M. Nan, F. Matto, C. Venzal, J. M. Rivero, R. |
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Buroni, F. |
| title |
Coenurosis in cattle in Uruguay |
| title_short |
Coenurosis in cattle in Uruguay |
| title_full |
Coenurosis in cattle in Uruguay |
| title_fullStr |
Coenurosis in cattle in Uruguay |
| title_full_unstemmed |
Coenurosis in cattle in Uruguay |
| title_sort |
coenurosis in cattle in uruguay |
| description |
Coenurosis is a disease caused by Coenurus cerebralis, the larval stage of Taenia multiceps. Ruminants, equids, swine and even humans can be affected developing neurological symptoms. This work reports C. cerebralis infection diagnosed by macroscopic and histologic lesions and molecular tools (PCR/ sequencing) in two heifers in Uruguay. Two 2-3 years old Hereford heifers were necropsied (case A and B). Both animals presented progressive deterioration, ataxia, circling and head deviation. Grossly, in case A, a cystic vesicle protruding from the left hemisphere was observed. The vesicle was broken spontaneously while the encephalon was retrieved from the cranial cavity. On case B, the whole cystic vesicle was retrieved from the right hemisphere. Through the translucent wall´s vesicle was possible to visualize whitish scolices attached to the inner layer. Histopathology of the central nervous system revealed an inflammatory reaction on the cyst adjacent tissue, characterized by the presence of macrophages, eosinophils, lymphocytes and giant cells. The vesicle of case B was dissected and a small piece was used for DNA extraction. By PCR, a fragment of nad1 gene was amplified and sent for direct sequencing. The obtained sequence was compared with the available sequences registered on GenBank using the BLAST tool. The result revealed a 99% homology with sequences registered as T. multiceps. Therefore, this work confirmed that bovine coenurosis is present in Uruguay. |
| publisher |
Sociedad de Medicina Veterinaria del Uruguay (SMVU) |
| publishDate |
2017 |
| url |
https://www.revistasmvu.com.uy/index.php/smvu/article/view/99 |
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