Brucella abortus significación de los títulos aglutinantes en bovinos de carne del Uruguay
A sample of 738 adult beef cows vaccinated with strain 19 was studied, the objective was to know the significance of agglutination titers. The Plate Agglutination test showed 449 (60.84 %) sera with titers, 32 (4.34 %) were suspicious and none was positive. The Tube Agglutination test gave similar results. The Rose Bengal test disclosed 42 (5.64 0/0) positive sera; from these sera two were positive to the Mercaptoethanol test end one of the last ones was positive to the Complement Fixation test too. All the other sera were negative to these tests. From 1280 Agar Dextrose serum plates (with inhibitors) seeded with samples from udder and limph nodes of 64 cows selected as possible infected, none Brucella abortus isolating were obtained. The authors suggest that most of the agglutination titers < 200 i,a.u. represent in Uruguay residuals vaccination antibodies and they claim to employ a more usefull diagnostic proceduns like the Rose Bengal test as screening and complemented with a more specific one.
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Sociedad de Medicina Veterinaria del Uruguay (SMVU)
1987
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rev-smvu-uy-article-9152023-06-29T18:48:45Z Brucella abortus significación de los títulos aglutinantes en bovinos de carne del Uruguay Bermudez, J. Barriola, J. Errico, F. Buchelli, D. Bovino Brucella abortus Diagnóstico serológico Catlle Brucella abortus Serological diagnosis A sample of 738 adult beef cows vaccinated with strain 19 was studied, the objective was to know the significance of agglutination titers. The Plate Agglutination test showed 449 (60.84 %) sera with titers, 32 (4.34 %) were suspicious and none was positive. The Tube Agglutination test gave similar results. The Rose Bengal test disclosed 42 (5.64 0/0) positive sera; from these sera two were positive to the Mercaptoethanol test end one of the last ones was positive to the Complement Fixation test too. All the other sera were negative to these tests. From 1280 Agar Dextrose serum plates (with inhibitors) seeded with samples from udder and limph nodes of 64 cows selected as possible infected, none Brucella abortus isolating were obtained. The authors suggest that most of the agglutination titers < 200 i,a.u. represent in Uruguay residuals vaccination antibodies and they claim to employ a more usefull diagnostic proceduns like the Rose Bengal test as screening and complemented with a more specific one. Los autores efectúan un estudio serológico y bacteriológico sobre 738 bovinos hembras de raza de carne vacunados con cepa 19 con el objetivo de determinar la significación de los títulos aglutinantes. La prueba de Aglutinación en Placa señala 449 (60.84 %) sueros con título, de ellas 32 (4.34 %) como sospechosos y ninguno como positivo. La Prueba de Aglutinación en Tubo señala resultados similares. Con el Rosa Bengala se obtuvieron 42 sueros positivos (5.69 %) de los que dos fueron positivos al Mercaptoetanol y de ellos solo uno a la Fijación de Complemento. Los restantes sueros todos fueron negativos a dichas pruebas. A partir de órganos (ubre y grupos ganglionares) de 64 animales seleccionados por su serología "sospechosos", se sembraron 1280 placas de Agar Dextrosa suero con inhibidores de los que no se obtuvo ningún aislamiento de Brucella abortus. Los autores concluyen que los títulos aglutinantes menores de 200 u.i,a., responden en su mayoría a anticuerpos residuales de vacunación por lo que es necesario emplear otro procedimiento diagnóstico como la Prueba de Rosa Bengala en función de descarte complementada con otra prueba más específica. Sociedad de Medicina Veterinaria del Uruguay (SMVU) 1987-01-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed article. Artículo evaluado por pares. Artigo revisado por pares. application/pdf https://www.revistasmvu.com.uy/index.php/smvu/article/view/915 Veterinaria (Montevideo); Vol. 23 No. 96 (1987): Enero - marzo; 21-23 Veterinaria (Montevideo); Vol. 23 Núm. 96 (1987): Enero - marzo; 21-23 Veterinaria (Montevideo); v. 23 n. 96 (1987): Enero - marzo; 21-23 1688-4809 0376-4362 spa https://www.revistasmvu.com.uy/index.php/smvu/article/view/915/568 Derechos de autor 1987 Sociedad de Medicina Veterinaria del Uruguay https://creativecommons.org/licenses/by-nc/4.0/deed.es |
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Uruguay |
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Biblioteca de la Facultad de Veterinaria de la UDELAR de UY |
| language |
spa |
| format |
Digital |
| author |
Bermudez, J. Barriola, J. Errico, F. Buchelli, D. |
| spellingShingle |
Bermudez, J. Barriola, J. Errico, F. Buchelli, D. Brucella abortus significación de los títulos aglutinantes en bovinos de carne del Uruguay |
| author_facet |
Bermudez, J. Barriola, J. Errico, F. Buchelli, D. |
| author_sort |
Bermudez, J. |
| title |
Brucella abortus significación de los títulos aglutinantes en bovinos de carne del Uruguay |
| title_short |
Brucella abortus significación de los títulos aglutinantes en bovinos de carne del Uruguay |
| title_full |
Brucella abortus significación de los títulos aglutinantes en bovinos de carne del Uruguay |
| title_fullStr |
Brucella abortus significación de los títulos aglutinantes en bovinos de carne del Uruguay |
| title_full_unstemmed |
Brucella abortus significación de los títulos aglutinantes en bovinos de carne del Uruguay |
| title_sort |
brucella abortus significación de los títulos aglutinantes en bovinos de carne del uruguay |
| description |
A sample of 738 adult beef cows vaccinated with strain 19 was studied, the objective was to know the significance of agglutination titers.
The Plate Agglutination test showed 449 (60.84 %) sera with titers, 32 (4.34 %) were suspicious and none was positive. The Tube Agglutination test gave similar results. The Rose Bengal test disclosed 42 (5.64 0/0) positive sera; from these sera two were positive to the Mercaptoethanol test end one of the last ones was positive to the Complement Fixation test too. All the other sera were negative to these tests. From 1280 Agar Dextrose serum plates (with inhibitors) seeded with samples from udder and limph nodes of 64 cows selected as possible infected, none Brucella abortus isolating were obtained.
The authors suggest that most of the agglutination titers < 200 i,a.u. represent in Uruguay residuals vaccination antibodies and they claim to employ a more usefull diagnostic proceduns like the Rose Bengal test as screening and complemented with a more specific one. |
| publisher |
Sociedad de Medicina Veterinaria del Uruguay (SMVU) |
| publishDate |
1987 |
| url |
https://www.revistasmvu.com.uy/index.php/smvu/article/view/915 |
| work_keys_str_mv |
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