Identification of Genomic Sequences (GSC) in Creole Bovines of Uruguay: Paper presented at the III Ibero-American Symposium on the Conservation of Local Animal Genetic Resources and Sustainable Rural Development

Identification of Genomic Sequences (GSC) in Creole Bovines of Uruguay: Paper presented at the III Ibero-American Symposium on the Conservation of Local Animal Genetic Resources and Sustainable Rural Development

Uruguayan Creole cattle is taking down to breeds introduced in the American continent around the XVI century. The cytogenetic characterization has showed: a) absence of Y acrocentric chromosome of afroasiatic origin, so there was no introgresion of cebuine breeds; b) robertsonian translocation (robl;29) with an incidence of 4%. It is considered an important rearrangement in chromosome evolution of the Bovidae family, that it is presented in ancestral breeds. Loss of a determinate DNA alphoid sequences (1) rich in CG was found during the evolution of the monocentric translocation. It is proposed to search CCGG sequences among cyto-molecular and genomic level in Creole normal and heterocygous from 1;29, to advance in the study of its chromatin. The restriction enzyme RE Mspl (0,3U/ul) was used to digest chromosome DNA. After this, CBG banding and stainning with propidium iodide were used to take a differential stain of the centromeric chromatin of the 1;29 in front of its homologous and the rest of the chromosomes. A particular chromatin configuration is showed. A light fluorescence is also shown in the 30 pairs of chromosome. The same restriction enzyme is used to digest the genomic DNA of a female heterozygous of robl;29 and a pool of fifteen DNA selected to their high variability and normal karyotype. The samples are run in polyacrilamide desnaturalized gel (6%), and differential bands were stained with silver nitrate. The heterozygous female expressed 43% of alike bands with the population sample, but not own bands is shown. Possible reorganization of chromatin connected wuth evolutive divergents is proposed.

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Main Authors: Postiglioni, A., Rincón, G., Llambí, S., Armstrong, E., Arruga, M. V.
Format: Digital revista
Language:spa
Published: Sociedad de Medicina Veterinaria del Uruguay (SMVU) 2004
Online Access:https://www.revistasmvu.com.uy/index.php/smvu/article/view/484
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id rev-smvu-uy-article-484
record_format ojs
institution UDELAR
collection OJS
country Uruguay
countrycode UY
component Revista
access En linea
databasecode rev-smvu-uy
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Veterinaria de la UDELAR de UY
language spa
format Digital
author Postiglioni, A.
Rincón, G.
Llambí, S.
Armstrong, E.
Arruga, M. V.
spellingShingle Postiglioni, A.
Rincón, G.
Llambí, S.
Armstrong, E.
Arruga, M. V.
Identification of Genomic Sequences (GSC) in Creole Bovines of Uruguay: Paper presented at the III Ibero-American Symposium on the Conservation of Local Animal Genetic Resources and Sustainable Rural Development
author_facet Postiglioni, A.
Rincón, G.
Llambí, S.
Armstrong, E.
Arruga, M. V.
author_sort Postiglioni, A.
title Identification of Genomic Sequences (GSC) in Creole Bovines of Uruguay: Paper presented at the III Ibero-American Symposium on the Conservation of Local Animal Genetic Resources and Sustainable Rural Development
title_short Identification of Genomic Sequences (GSC) in Creole Bovines of Uruguay: Paper presented at the III Ibero-American Symposium on the Conservation of Local Animal Genetic Resources and Sustainable Rural Development
title_full Identification of Genomic Sequences (GSC) in Creole Bovines of Uruguay: Paper presented at the III Ibero-American Symposium on the Conservation of Local Animal Genetic Resources and Sustainable Rural Development
title_fullStr Identification of Genomic Sequences (GSC) in Creole Bovines of Uruguay: Paper presented at the III Ibero-American Symposium on the Conservation of Local Animal Genetic Resources and Sustainable Rural Development
title_full_unstemmed Identification of Genomic Sequences (GSC) in Creole Bovines of Uruguay: Paper presented at the III Ibero-American Symposium on the Conservation of Local Animal Genetic Resources and Sustainable Rural Development
title_sort identification of genomic sequences (gsc) in creole bovines of uruguay: paper presented at the iii ibero-american symposium on the conservation of local animal genetic resources and sustainable rural development
description Uruguayan Creole cattle is taking down to breeds introduced in the American continent around the XVI century. The cytogenetic characterization has showed: a) absence of Y acrocentric chromosome of afroasiatic origin, so there was no introgresion of cebuine breeds; b) robertsonian translocation (robl;29) with an incidence of 4%. It is considered an important rearrangement in chromosome evolution of the Bovidae family, that it is presented in ancestral breeds. Loss of a determinate DNA alphoid sequences (1) rich in CG was found during the evolution of the monocentric translocation. It is proposed to search CCGG sequences among cyto-molecular and genomic level in Creole normal and heterocygous from 1;29, to advance in the study of its chromatin. The restriction enzyme RE Mspl (0,3U/ul) was used to digest chromosome DNA. After this, CBG banding and stainning with propidium iodide were used to take a differential stain of the centromeric chromatin of the 1;29 in front of its homologous and the rest of the chromosomes. A particular chromatin configuration is showed. A light fluorescence is also shown in the 30 pairs of chromosome. The same restriction enzyme is used to digest the genomic DNA of a female heterozygous of robl;29 and a pool of fifteen DNA selected to their high variability and normal karyotype. The samples are run in polyacrilamide desnaturalized gel (6%), and differential bands were stained with silver nitrate. The heterozygous female expressed 43% of alike bands with the population sample, but not own bands is shown. Possible reorganization of chromatin connected wuth evolutive divergents is proposed.
publisher Sociedad de Medicina Veterinaria del Uruguay (SMVU)
publishDate 2004
url https://www.revistasmvu.com.uy/index.php/smvu/article/view/484
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spelling rev-smvu-uy-article-4842020-08-17T15:22:00Z Identification of Genomic Sequences (GSC) in Creole Bovines of Uruguay: Paper presented at the III Ibero-American Symposium on the Conservation of Local Animal Genetic Resources and Sustainable Rural Development Identificación de Secuencias Genómicas (CCGG) en Bovinos Criollos del Uruguay: Trabajo presentado al III Simposio Iberoamericano sobre la Conservación de los Recursos Zoogenéticos Locales y el Desarrollo Rural Sostenible Postiglioni, A. Rincón, G. Llambí, S. Armstrong, E. Arruga, M. V. Robl;29 Bovinos criollos Secuencias CG Robl;29 Creole cattle CG sequences Uruguayan Creole cattle is taking down to breeds introduced in the American continent around the XVI century. The cytogenetic characterization has showed: a) absence of Y acrocentric chromosome of afroasiatic origin, so there was no introgresion of cebuine breeds; b) robertsonian translocation (robl;29) with an incidence of 4%. It is considered an important rearrangement in chromosome evolution of the Bovidae family, that it is presented in ancestral breeds. Loss of a determinate DNA alphoid sequences (1) rich in CG was found during the evolution of the monocentric translocation. It is proposed to search CCGG sequences among cyto-molecular and genomic level in Creole normal and heterocygous from 1;29, to advance in the study of its chromatin. The restriction enzyme RE Mspl (0,3U/ul) was used to digest chromosome DNA. After this, CBG banding and stainning with propidium iodide were used to take a differential stain of the centromeric chromatin of the 1;29 in front of its homologous and the rest of the chromosomes. A particular chromatin configuration is showed. A light fluorescence is also shown in the 30 pairs of chromosome. The same restriction enzyme is used to digest the genomic DNA of a female heterozygous of robl;29 and a pool of fifteen DNA selected to their high variability and normal karyotype. The samples are run in polyacrilamide desnaturalized gel (6%), and differential bands were stained with silver nitrate. The heterozygous female expressed 43% of alike bands with the population sample, but not own bands is shown. Possible reorganization of chromatin connected wuth evolutive divergents is proposed. Los bovinos Criollos Uruguayos se consideran descendientes directos de razas introducidas en América durante la época colonial (siglo XVI), hoy naturalizadas. Su caracterización citogenética ha determinado: a) ausencia de cromosoma Y acrocéntrico de origen afroasiático, apoyando la no introgresión de razas cebuinas; b) presencia (4%) de la translocación robertsoniana (robl;29), destacado reordenamiento de la evolución cromosómica de la familia Bovidae, presente en posibles razas ancestrales. Se ha descrito pérdida de secuencias de ADN alfoides (1) rico en CG durante su evolución como translocación monocéntrica. En esta comunicación se plantea la búsqueda a nivel cito-molecular y genómico de secuencias CCGG en bovinos criollos normales y portadores de la robl;29 con el propósito de profundizar en el estudio de su cromatina. Se utiliza la enzima de restricción ER Mspl (0,3U/ul) para digerir el ADN cromosómico. Se aplica bandeo CBG y contra-coloración con yoduro de propidio obteniéndose una tinción diferencial de la cromatina centromérica del cromosoma 1;29, frente a sus homólogos y resto del cariotipo. Se plantea una configuración particular de esta cromatina. Los 30 pares de brazos cromosómicos se expresan con fluorescencia pálida luego del tratamiento. Se utiliza la misma enzima Mspl, para digerir el ADN genómico de una hembra portadora de la robl;29 y un "pool" de 15 ADNs seleccionados por su alta variabilidad y cariotipo normal. El producto se corre en gel de poliacrilamida no desnaturalizante (6%) teñido con nitrato de plata observándose un patrón de bandeo diferencial. La hembra portadora expresa un 43% de similitud con la muestra poblacional, no presentando bandas propias. Se plantean posibles reorganizaciones de la cromatina con probable implicancia en divergencias evolutivas. Sociedad de Medicina Veterinaria del Uruguay (SMVU) 2004-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://www.revistasmvu.com.uy/index.php/smvu/article/view/484 Veterinaria (Montevideo); Vol. 39 No. 155 - 156 (2004): April - December; 17-20 Veterinaria (Montevideo); Vol. 39 Núm. 155 - 156 (2004): Abril - Diciembre; 17-20 Veterinaria (Montevideo); v. 39 n. 155 - 156 (2004): Abril - Dezembro; 17-20 1688-4809 0376-4362 spa https://www.revistasmvu.com.uy/index.php/smvu/article/view/484/319 Derechos de autor 2004 Sociedad de Medicina Veterinaria del Uruguay

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