Molecular detection of Hepatozoon felis in anemic cats in Uruguay
Hepatozoonosis is a parasitic disease caused by Hepatozoon spp. In general, it has a subclinical progression, and its symptoms are associated with an induced immunodeficiency triggered by a concomitant disease. The aim of this study was to detect by PCR the presence of Hepatozoon spp. in domestic cats from Uruguay. A total of 230 blood samples were analyzed obtained in nine of the 19 departments of Uruguay. Only were included samples from cats with early diagnosis of clinical anemia. For molecular diagnosis, two PCRs targeting two overlapping regions of 18S rRNA gene for Hepatozoon spp. were carried out. Nine out of 230 samples were positive for Hepatozoon spp., resulting in an infection frequency of 3.91%. The obtained sequences revealed 99.10 to 100% identity with Hepatozoon felis sequences registered in GenBank. Moreover, the phylogenetic analysis showed that the sequences obtained in this study clustered, with a high Bootstrap support with sequences of H. felis from different continents. To our knowledge, this is the first report of H. felis infecting domestic cats in Uruguay.
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Sociedad de Medicina Veterinaria del Uruguay (SMVU)
2021
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rev-smvu-uy-article-2742021-05-21T17:18:47Z Molecular detection of Hepatozoon felis in anemic cats in Uruguay Detección molecular de Hepatozoon felis en gatos con anemia en Uruguay Bazzano, Valentín Freire, Jorge Armúa-Fernández, María T. Félix, María L. Carvalho, Luis A. Venzal, José Hepatozoonosis Hepatozoon felis PCR Gatos Uruguay Anemia Hepatozoonosis Hepatozoon felis PCR Cats Uruguay Anemia Hepatozoonosis is a parasitic disease caused by Hepatozoon spp. In general, it has a subclinical progression, and its symptoms are associated with an induced immunodeficiency triggered by a concomitant disease. The aim of this study was to detect by PCR the presence of Hepatozoon spp. in domestic cats from Uruguay. A total of 230 blood samples were analyzed obtained in nine of the 19 departments of Uruguay. Only were included samples from cats with early diagnosis of clinical anemia. For molecular diagnosis, two PCRs targeting two overlapping regions of 18S rRNA gene for Hepatozoon spp. were carried out. Nine out of 230 samples were positive for Hepatozoon spp., resulting in an infection frequency of 3.91%. The obtained sequences revealed 99.10 to 100% identity with Hepatozoon felis sequences registered in GenBank. Moreover, the phylogenetic analysis showed that the sequences obtained in this study clustered, with a high Bootstrap support with sequences of H. felis from different continents. To our knowledge, this is the first report of H. felis infecting domestic cats in Uruguay. La Hepatozoonosis felina es una enfermedad parasitaria causada por Hepatozoon spp., que generalmente cursa de manera subclínica y cuando se manifiesta lo hace en asociación con una inmunodeficiencia inducida por una enfermedad concomitante. El objetivo del presente estudio fue determinar la presencia de Hepatozoon spp. en gatos domésticos de Uruguay a través del diagnóstico mediante técnicas moleculares (PCR). Para ello se obtuvieron 230 muestras de sangre de gatos que previamente habían sido diagnosticados con anemia clínica, procedentes de nueve de los 19 departamentos del país. Para el diagnóstico molecular, dos PCRs independientes fueron realizadas amplificando dos fragmentos solapantes del gen 18S ARN ribosomal de Hepatozoon spp. Nueve de las 230 muestras resultaron positivas a Hepatozoon spp., siendo la frecuencia de infección del 3,91%. La identidad de las secuencias obtenidas fue entre 99,10 y 100% con secuencias de Hepatozoon felis disponibles en GenBank. Así mismo, el análisis filogenético demostró que estas secuencias obtenidas de los gatos de Uruguay se agrupan en un clado con alto soporte con secuencias de H. felis procedentes de diferentes continentes. En base a estos resultados, se reporta por primera vez la presencia de H. felis infectando gatos domésticos en Uruguay. Sociedad de Medicina Veterinaria del Uruguay (SMVU) 2021-04-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed article. Artículo evaluado por pares. Artigo revisado por pares. application/pdf https://www.revistasmvu.com.uy/index.php/smvu/article/view/274 Veterinaria (Montevideo); Vol. 57 No. 215 (2021): Enero - junio 2021; e20215721504 Veterinaria (Montevideo); Vol. 57 Núm. 215 (2021): Enero - junio 2021; e20215721504 Veterinaria (Montevideo); v. 57 n. 215 (2021): Enero - junio 2021; e20215721504 1688-4809 0376-4362 spa https://www.revistasmvu.com.uy/index.php/smvu/article/view/274/516 Derechos de autor 2021 Sociedad de Medicina Veterinaria del Uruguay |
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Uruguay |
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Biblioteca de la Facultad de Veterinaria de la UDELAR de UY |
| language |
spa |
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| author |
Bazzano, Valentín Freire, Jorge Armúa-Fernández, María T. Félix, María L. Carvalho, Luis A. Venzal, José |
| spellingShingle |
Bazzano, Valentín Freire, Jorge Armúa-Fernández, María T. Félix, María L. Carvalho, Luis A. Venzal, José Molecular detection of Hepatozoon felis in anemic cats in Uruguay |
| author_facet |
Bazzano, Valentín Freire, Jorge Armúa-Fernández, María T. Félix, María L. Carvalho, Luis A. Venzal, José |
| author_sort |
Bazzano, Valentín |
| title |
Molecular detection of Hepatozoon felis in anemic cats in Uruguay |
| title_short |
Molecular detection of Hepatozoon felis in anemic cats in Uruguay |
| title_full |
Molecular detection of Hepatozoon felis in anemic cats in Uruguay |
| title_fullStr |
Molecular detection of Hepatozoon felis in anemic cats in Uruguay |
| title_full_unstemmed |
Molecular detection of Hepatozoon felis in anemic cats in Uruguay |
| title_sort |
molecular detection of hepatozoon felis in anemic cats in uruguay |
| description |
Hepatozoonosis is a parasitic disease caused by Hepatozoon spp. In general, it has a subclinical progression, and its symptoms are associated with an induced immunodeficiency triggered by a concomitant disease. The aim of this study was to detect by PCR the presence of Hepatozoon spp. in domestic cats from Uruguay. A total of 230 blood samples were analyzed obtained in nine of the 19 departments of Uruguay. Only were included samples from cats with early diagnosis of clinical anemia. For molecular diagnosis, two PCRs targeting two overlapping regions of 18S rRNA gene for Hepatozoon spp. were carried out. Nine out of 230 samples were positive for Hepatozoon spp., resulting in an infection frequency of 3.91%. The obtained sequences revealed 99.10 to 100% identity with Hepatozoon felis sequences registered in GenBank. Moreover, the phylogenetic analysis showed that the sequences obtained in this study clustered, with a high Bootstrap support with sequences of H. felis from different continents. To our knowledge, this is the first report of H. felis infecting domestic cats in Uruguay. |
| publisher |
Sociedad de Medicina Veterinaria del Uruguay (SMVU) |
| publishDate |
2021 |
| url |
https://www.revistasmvu.com.uy/index.php/smvu/article/view/274 |
| work_keys_str_mv |
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1781886131612155904 |