The restriction in the time of access to forage in ovine fed a high quality pasture affect groups of ruminal microorganisms
The effects of restricting the time of access to forage and of the time from the beginning of the intake on different groups of ruminal microbes were studied. Six fistulized wethers, allocated in individual cages, consuming only fresh forage (88% legumes) were separated in 2 groups: 24h and 6h (forage available during all the day and 6 h/d, respectively). After 30 days of adaptation, ruminal content samples (fluid + solid) were extracted to evaluate the microbiota of each group (24h vs. 6h). In animals with restricted access to forage (6h group) the microbiota was also compared among 0, 4 and 8 hours from the beginning of the meal. The samples were analyzed by FISH (Fluorescence in situ hybridization), using probes targeting the Domain Bacteria (Eubacteria), fibrolytic (Ruminococcus albus and flavefaciens, lactate-producing (Streptococcus spp.) and lactate-consuming (Megasphaera elsdenii, Propionibacterium spp., Selenomona ruminantium) bacterias. The ruminal pH was not affected by the restriction, but R. albus, R. flavefaciens and S. ruminantum were more abundant for the 24h group. Within the animals under restriction the number of Eubacterias tended to be lower al hours 4 and 8, when ruminal pH was lower too, regarding to the hour 0, possibly due to the entry of fresh food. There were not differences between bacteria groups due to the sampling time were not observed.
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Sociedad de Medicina Veterinaria del Uruguay (SMVU)
2014
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rev-smvu-uy-article-2562022-12-06T18:58:05Z The restriction in the time of access to forage in ovine fed a high quality pasture affect groups of ruminal microorganisms La restricción en el tiempo de acceso al forraje en ovinos alimentados con pastura de buena calidad afecta algunos grupos microbianos ruminales Pérez-Ruchel, A. Repetto, J. L. Fraga, M. Perelmuter, K. Zunino, P. Cajarville, C. Pastura templada Microbiota ruminal Bacterias fibrolíticas Selenomonas ruminantium Temperate pasture Ruminalmicrobiota Fibrolytic bacteria Selenomonas ruminantium The effects of restricting the time of access to forage and of the time from the beginning of the intake on different groups of ruminal microbes were studied. Six fistulized wethers, allocated in individual cages, consuming only fresh forage (88% legumes) were separated in 2 groups: 24h and 6h (forage available during all the day and 6 h/d, respectively). After 30 days of adaptation, ruminal content samples (fluid + solid) were extracted to evaluate the microbiota of each group (24h vs. 6h). In animals with restricted access to forage (6h group) the microbiota was also compared among 0, 4 and 8 hours from the beginning of the meal. The samples were analyzed by FISH (Fluorescence in situ hybridization), using probes targeting the Domain Bacteria (Eubacteria), fibrolytic (Ruminococcus albus and flavefaciens, lactate-producing (Streptococcus spp.) and lactate-consuming (Megasphaera elsdenii, Propionibacterium spp., Selenomona ruminantium) bacterias. The ruminal pH was not affected by the restriction, but R. albus, R. flavefaciens and S. ruminantum were more abundant for the 24h group. Within the animals under restriction the number of Eubacterias tended to be lower al hours 4 and 8, when ruminal pH was lower too, regarding to the hour 0, possibly due to the entry of fresh food. There were not differences between bacteria groups due to the sampling time were not observed. Se estudiaron los efectos de restringir el tiempo de acceso al forraje y del tiempo desde el inicio de la ingesta, sobre diferentes grupos bacterianos ruminales. Seis borregos fistulados, alojados en jaulas individuales, consumiendo únicamente forraje fresco (88% leguminosas) fueron distribuidos en 2 grupos: 24h y 6h (forraje disponible todo el día y 6 h/día, respectivamente). Luego de 30 días de adaptación, se extrajeron muestras de contenido ruminal (líquido + sólido) para estudiar la microbiota de cada grupo (24h vs. 6h). En los animales con acceso restringido al forraje (grupo 6h) se comparó también la microbiota entre las horas 0, 4 y 8 desde el inicio de la ingesta. Las muestras se analizaron por FISH (Fluorescence in situ hybridization), utilizando sondas para el Dominio Bacteria (Eubacterias), bacterias fibrolíticas (Ruminococcus albusyflavefaciens, productoras de lactato (Streptococcus spp.) y consumidoras de lactato (Megasphaera elsdenii, Propionibacterium spp., Selenomonas ruminantium. El pH ruminal no fue afectado por la restricción, pero R. albus, R. flavefaciens y S. ruminantium fueron más abundantes para el grupo 24h. Dentro de los animales sometidos a restricción las Eubacterias tendieron a ser menos abundantes a las horas 4 y 8, cuando el pH ruminal también fue menor respecto a la hora 0, posiblemente debido al ingreso de alimento fresco. No se observaron diferencias entre grupos bacterianos debidas al horario de muestreo. Sociedad de Medicina Veterinaria del Uruguay (SMVU) 2014-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed article. Artículo evaluado por pares. Artigo revisado por pares. application/pdf https://www.revistasmvu.com.uy/index.php/smvu/article/view/256 Veterinaria (Montevideo); Vol. 50 No. 194 (2014): June; 22-33 Veterinaria (Montevideo); Vol. 50 Núm. 194 (2014): Junio; 22-33 Veterinaria (Montevideo); v. 50 n. 194 (2014): Junho; 22-33 1688-4809 0376-4362 spa https://www.revistasmvu.com.uy/index.php/smvu/article/view/256/172 Derechos de autor 2014 Sociedad de Medicina Veterinaria del Uruguay https://creativecommons.org/licenses/by-nc/4.0 |
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| author |
Pérez-Ruchel, A. Repetto, J. L. Fraga, M. Perelmuter, K. Zunino, P. Cajarville, C. |
| spellingShingle |
Pérez-Ruchel, A. Repetto, J. L. Fraga, M. Perelmuter, K. Zunino, P. Cajarville, C. The restriction in the time of access to forage in ovine fed a high quality pasture affect groups of ruminal microorganisms |
| author_facet |
Pérez-Ruchel, A. Repetto, J. L. Fraga, M. Perelmuter, K. Zunino, P. Cajarville, C. |
| author_sort |
Pérez-Ruchel, A. |
| title |
The restriction in the time of access to forage in ovine fed a high quality pasture affect groups of ruminal microorganisms |
| title_short |
The restriction in the time of access to forage in ovine fed a high quality pasture affect groups of ruminal microorganisms |
| title_full |
The restriction in the time of access to forage in ovine fed a high quality pasture affect groups of ruminal microorganisms |
| title_fullStr |
The restriction in the time of access to forage in ovine fed a high quality pasture affect groups of ruminal microorganisms |
| title_full_unstemmed |
The restriction in the time of access to forage in ovine fed a high quality pasture affect groups of ruminal microorganisms |
| title_sort |
restriction in the time of access to forage in ovine fed a high quality pasture affect groups of ruminal microorganisms |
| description |
The effects of restricting the time of access to forage and of the time from the beginning of the intake on different groups of ruminal microbes were studied. Six fistulized wethers, allocated in individual cages, consuming only fresh forage (88% legumes) were separated in 2 groups: 24h and 6h (forage available during all the day and 6 h/d, respectively). After 30 days of adaptation, ruminal content samples (fluid + solid) were extracted to evaluate the microbiota of each group (24h vs. 6h). In animals with restricted access to forage (6h group) the microbiota was also compared among 0, 4 and 8 hours from the beginning of the meal. The samples were analyzed by FISH (Fluorescence in situ hybridization), using probes targeting the Domain Bacteria (Eubacteria), fibrolytic (Ruminococcus albus and flavefaciens, lactate-producing (Streptococcus spp.) and lactate-consuming (Megasphaera elsdenii, Propionibacterium spp., Selenomona ruminantium) bacterias. The ruminal pH was not affected by the restriction, but R. albus, R. flavefaciens and S. ruminantum were more abundant for the 24h group. Within the animals under restriction the number of Eubacterias tended to be lower al hours 4 and 8, when ruminal pH was lower too, regarding to the hour 0, possibly due to the entry of fresh food. There were not differences between bacteria groups due to the sampling time were not observed. |
| publisher |
Sociedad de Medicina Veterinaria del Uruguay (SMVU) |
| publishDate |
2014 |
| url |
https://www.revistasmvu.com.uy/index.php/smvu/article/view/256 |
| work_keys_str_mv |
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