Characterization of the ruminal bacterial microbiota of a grazing bovine through classical and cultivation-independent techniques
Ruminal microbiota has conferred ruminants the ability to utilize cellulose present in vegetal material. Fiber breakdown provides energy sources to ruminants and the microbial biomass represents the main protein source for grazing animal. Rumen bacterial community is composed by hundreds of species and is distributed in the particulate solid phase, in ruminal fluid and to a lesser extent associated to the nuninal epithelium. Only a small portion of the mainly-anaerobic ruminal microbiota can normally be cultured. Molecular methods have shed light in the study of microbial diversity without cultivation. The objectives of this work were to characterize the cultivable and non-cultivable ruminal bacterial community associated with solid and fluid rumen content fractions of a grazing bovine and to isolate and identify bacteria that are able to grow onto a culture media with pure cellulose as the main energy and carbon sources. Sixteen isolates were identified including members of Butyrivibrio, Pseudobutyrivi- facbrio, Succinivibrio and Selenemonas genera. Four bacterial isolates were representatives of new genera and species. As far as we are concerned, this is the first local approach focused on the identification and characterization of ruminal native microorganisms using classic bacteriology and molecular techniques.
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Sociedad de Medicina Veterinaria del Uruguay (SMVU)
2013
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rev-smvu-uy-article-2182019-10-30T18:50:38Z Characterization of the ruminal bacterial microbiota of a grazing bovine through classical and cultivation-independent techniques Caracterización de la microbiota bacteriana ruminal de un bovino a pastoreo mediante técnicas clásicas e independientes del cultivo Fraga, M. Perelmuter, K. Valencia, M. J. Cajarville, C. Zunino, P. Microbiota bacteriana ruminal FISH Pastoreo Ruminal bacterial community FISH Grazing animals Ruminal microbiota has conferred ruminants the ability to utilize cellulose present in vegetal material. Fiber breakdown provides energy sources to ruminants and the microbial biomass represents the main protein source for grazing animal. Rumen bacterial community is composed by hundreds of species and is distributed in the particulate solid phase, in ruminal fluid and to a lesser extent associated to the nuninal epithelium. Only a small portion of the mainly-anaerobic ruminal microbiota can normally be cultured. Molecular methods have shed light in the study of microbial diversity without cultivation. The objectives of this work were to characterize the cultivable and non-cultivable ruminal bacterial community associated with solid and fluid rumen content fractions of a grazing bovine and to isolate and identify bacteria that are able to grow onto a culture media with pure cellulose as the main energy and carbon sources. Sixteen isolates were identified including members of Butyrivibrio, Pseudobutyrivi- facbrio, Succinivibrio and Selenemonas genera. Four bacterial isolates were representatives of new genera and species. As far as we are concerned, this is the first local approach focused on the identification and characterization of ruminal native microorganisms using classic bacteriology and molecular techniques. La microbiota simbionte ruminal ha conferido a los rumiantes la ventaja evolutiva de poder aprovechar la fibra vegetal a traves de su metabolismo fermentativo. La fermentación de la fibra en el rumen provee de fuentes de energía mientras que la biomasa microbiana aporta la principal fuente de proteínas. La comunidad bacteriana ruminal comprende varios cientos de especies bacterianas y esta distribuida en la fase sólida del contenido ruminal, en el fluido ruminal y en una menor medida asociada al epitelio. Solo una fracción de la microbiota ruminal, mayoritariamente anaerobia, puede ser cultivada y el advenimiento de los métodos moleculares ha permitido conocer la diversidad microbiana sin la necesidad de cultivo. Los objetivos de este trabajo fueron caracterizar la microbiota bacteriana ruminal cultivable y no cultivable asociada a las fracciones sólida y líquida del contenido ruminal de un bovino a pastoreo y aislar y clasificar bacterias capaces de crecer en un medio de cultivo con celulosa como principal fuente de carbono y energía. Para evaluar la microbiota cultivable se utilizaron medios de cultivo artificiales mientras que el análisis de la comunidad microbiana total se realizó por medio de la técnica de Fluorescent in situ hybridization (FISH). Se identificaron 16 aislamientos incluyendo miembros de los géneros Butyrivibrio, Pseudobutyrivibrio, Succinivibrio y Selenomonas ademas de otros 4 que representan nuevas especies y géneros bacterianos. Este trabajo representa una primera aproximación en el país dirigida a aislar e identificar microorganismos ruminales por medio de técnicas bacteriológicas y moleculares. Sociedad de Medicina Veterinaria del Uruguay (SMVU) 2013-03-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed article. Artículo evaluado por pares. Artigo revisado por pares. application/pdf https://www.revistasmvu.com.uy/index.php/smvu/article/view/218 Veterinaria (Montevideo); Vol. 49 No. 189 (2013): March; 26-38 Veterinaria (Montevideo); Vol. 49 Núm. 189 (2013): Marzo; 26-38 Veterinaria (Montevideo); v. 49 n. 189 (2013): Março; 26-38 1688-4809 0376-4362 spa https://www.revistasmvu.com.uy/index.php/smvu/article/view/218/144 Derechos de autor 2013 Sociedad de Medicina Veterinaria del Uruguay |
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| author |
Fraga, M. Perelmuter, K. Valencia, M. J. Cajarville, C. Zunino, P. |
| spellingShingle |
Fraga, M. Perelmuter, K. Valencia, M. J. Cajarville, C. Zunino, P. Characterization of the ruminal bacterial microbiota of a grazing bovine through classical and cultivation-independent techniques |
| author_facet |
Fraga, M. Perelmuter, K. Valencia, M. J. Cajarville, C. Zunino, P. |
| author_sort |
Fraga, M. |
| title |
Characterization of the ruminal bacterial microbiota of a grazing bovine through classical and cultivation-independent techniques |
| title_short |
Characterization of the ruminal bacterial microbiota of a grazing bovine through classical and cultivation-independent techniques |
| title_full |
Characterization of the ruminal bacterial microbiota of a grazing bovine through classical and cultivation-independent techniques |
| title_fullStr |
Characterization of the ruminal bacterial microbiota of a grazing bovine through classical and cultivation-independent techniques |
| title_full_unstemmed |
Characterization of the ruminal bacterial microbiota of a grazing bovine through classical and cultivation-independent techniques |
| title_sort |
characterization of the ruminal bacterial microbiota of a grazing bovine through classical and cultivation-independent techniques |
| description |
Ruminal microbiota has conferred ruminants the ability to utilize cellulose present in vegetal material. Fiber breakdown provides energy sources to ruminants and the microbial biomass represents the main protein source for grazing animal. Rumen bacterial community is composed by hundreds of species and is distributed in the particulate solid phase, in ruminal fluid and to a lesser extent associated to the nuninal epithelium. Only a small portion of the mainly-anaerobic ruminal microbiota can normally be cultured. Molecular methods have shed light in the study of microbial diversity without cultivation. The objectives of this work were to characterize the cultivable and non-cultivable ruminal bacterial community associated with solid and fluid rumen content fractions of a grazing bovine and to isolate and identify bacteria that are able to grow onto a culture media with pure cellulose as the main energy and carbon sources. Sixteen isolates were identified including members of Butyrivibrio, Pseudobutyrivi- facbrio, Succinivibrio and Selenemonas genera. Four bacterial isolates were representatives of new genera and species. As far as we are concerned, this is the first local approach focused on the identification and characterization of ruminal native microorganisms using classic bacteriology and molecular techniques. |
| publisher |
Sociedad de Medicina Veterinaria del Uruguay (SMVU) |
| publishDate |
2013 |
| url |
https://www.revistasmvu.com.uy/index.php/smvu/article/view/218 |
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