Virus prevalence and mixed infections in yellow passion fruit (Passiflora edulis f. flavicarpa) crops in Valle del Cauca, Colombia
Yellow passion fruit crop is affected by multiple phytosanitary problems. Among the main ones are viruses of the genera Potyvirus (soybean mosaic virus, SMV), Cucumovirus (cucumber mosaic virus, CMV), Tymovirus (tymovirus passiflorae, PYMV) and Begomovirus (passionfruit leaf distortion virus, PLDV). Information about the prevalence and interaction between these viruses is scarce. The objective of this study was to verify the prevalence and identify the types of mixed viral infections of potyvirus, cucumovirus, tymovirus, and begomovirus in passion fruit crops in Valle del Cauca, Colombia. Passion fruit leaf samples with symptoms were collected and their nucleic acids were purified. Virus identification was performed by PCR using universal primers by viral genus, and specific primers for the SMV, CMV, PFYMV and PLDV viruses. The incidence of each virus by municipality was analyzed and the severity of the symptoms was compared with the diagnosis made. In total, 66 samples were collected in the municipalities of Toro, Dagua, Roldanillo, Bolívar, La Unión and El Cerrito. It was established that the most prevalent viruses are PLDV followed by SMV and CMV. The presence of PYMV was not detected in the samples analyzed. The presence of double and triple infections among potyvirus, cucumovirus and begomovirus were identified for the first time, finding greater severity of symptoms with a greater number of viruses identified per sample. This research provides key results for the design of control strategies for viral diseases in passion fruit.
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Universidad de Ciencias Aplicadas y Ambientales U.D.C.A
2024
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Ceballos-Burgos, Francy Jhoana Pérez-López, Jorge Iván Nieto-Cardenas , Andres Felipe Rivera-Toro, Diana Marcela Ocampo, Jhon Albeiro Lopez-Lopez, Karina Vaca-Vaca, Juan Carlos |
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Ceballos-Burgos, Francy Jhoana Pérez-López, Jorge Iván Nieto-Cardenas , Andres Felipe Rivera-Toro, Diana Marcela Ocampo, Jhon Albeiro Lopez-Lopez, Karina Vaca-Vaca, Juan Carlos Virus prevalence and mixed infections in yellow passion fruit (Passiflora edulis f. flavicarpa) crops in Valle del Cauca, Colombia |
| author_facet |
Ceballos-Burgos, Francy Jhoana Pérez-López, Jorge Iván Nieto-Cardenas , Andres Felipe Rivera-Toro, Diana Marcela Ocampo, Jhon Albeiro Lopez-Lopez, Karina Vaca-Vaca, Juan Carlos |
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Ceballos-Burgos, Francy Jhoana |
| title |
Virus prevalence and mixed infections in yellow passion fruit (Passiflora edulis f. flavicarpa) crops in Valle del Cauca, Colombia |
| title_short |
Virus prevalence and mixed infections in yellow passion fruit (Passiflora edulis f. flavicarpa) crops in Valle del Cauca, Colombia |
| title_full |
Virus prevalence and mixed infections in yellow passion fruit (Passiflora edulis f. flavicarpa) crops in Valle del Cauca, Colombia |
| title_fullStr |
Virus prevalence and mixed infections in yellow passion fruit (Passiflora edulis f. flavicarpa) crops in Valle del Cauca, Colombia |
| title_full_unstemmed |
Virus prevalence and mixed infections in yellow passion fruit (Passiflora edulis f. flavicarpa) crops in Valle del Cauca, Colombia |
| title_sort |
virus prevalence and mixed infections in yellow passion fruit (passiflora edulis f. flavicarpa) crops in valle del cauca, colombia |
| description |
Yellow passion fruit crop is affected by multiple phytosanitary problems. Among the main ones are viruses of the genera Potyvirus (soybean mosaic virus, SMV), Cucumovirus (cucumber mosaic virus, CMV), Tymovirus (tymovirus passiflorae, PYMV) and Begomovirus (passionfruit leaf distortion virus, PLDV). Information about the prevalence and interaction between these viruses is scarce. The objective of this study was to verify the prevalence and identify the types of mixed viral infections of potyvirus, cucumovirus, tymovirus, and begomovirus in passion fruit crops in Valle del Cauca, Colombia. Passion fruit leaf samples with symptoms were collected and their nucleic acids were purified. Virus identification was performed by PCR using universal primers by viral genus, and specific primers for the SMV, CMV, PFYMV and PLDV viruses. The incidence of each virus by municipality was analyzed and the severity of the symptoms was compared with the diagnosis made. In total, 66 samples were collected in the municipalities of Toro, Dagua, Roldanillo, Bolívar, La Unión and El Cerrito. It was established that the most prevalent viruses are PLDV followed by SMV and CMV. The presence of PYMV was not detected in the samples analyzed. The presence of double and triple infections among potyvirus, cucumovirus and begomovirus were identified for the first time, finding greater severity of symptoms with a greater number of viruses identified per sample. This research provides key results for the design of control strategies for viral diseases in passion fruit. |
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Universidad de Ciencias Aplicadas y Ambientales U.D.C.A |
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2024 |
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https://revistas.udca.edu.co/index.php/ruadc/article/view/2364 |
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rev-ruadc-co-article-23642024-07-10T19:35:02Z Virus prevalence and mixed infections in yellow passion fruit (Passiflora edulis f. flavicarpa) crops in Valle del Cauca, Colombia Prevalencia e infección mixta de virus en cultivos de maracuyá amarillo (Passiflora edulis f. flavicarpa) en el Valle del Cauca, Colombia Ceballos-Burgos, Francy Jhoana Pérez-López, Jorge Iván Nieto-Cardenas , Andres Felipe Rivera-Toro, Diana Marcela Ocampo, Jhon Albeiro Lopez-Lopez, Karina Vaca-Vaca, Juan Carlos Begomovirus Cucumovirus Identificación de virus Potyvirus Virus vegetales Begomovirus Cucumovirus Plant viruses Potyvirus Virus identification Maracujá Potyvirus Cucumovirus Begomovirus Infecções mistas Yellow passion fruit crop is affected by multiple phytosanitary problems. Among the main ones are viruses of the genera Potyvirus (soybean mosaic virus, SMV), Cucumovirus (cucumber mosaic virus, CMV), Tymovirus (tymovirus passiflorae, PYMV) and Begomovirus (passionfruit leaf distortion virus, PLDV). Information about the prevalence and interaction between these viruses is scarce. The objective of this study was to verify the prevalence and identify the types of mixed viral infections of potyvirus, cucumovirus, tymovirus, and begomovirus in passion fruit crops in Valle del Cauca, Colombia. Passion fruit leaf samples with symptoms were collected and their nucleic acids were purified. Virus identification was performed by PCR using universal primers by viral genus, and specific primers for the SMV, CMV, PFYMV and PLDV viruses. The incidence of each virus by municipality was analyzed and the severity of the symptoms was compared with the diagnosis made. In total, 66 samples were collected in the municipalities of Toro, Dagua, Roldanillo, Bolívar, La Unión and El Cerrito. It was established that the most prevalent viruses are PLDV followed by SMV and CMV. The presence of PYMV was not detected in the samples analyzed. The presence of double and triple infections among potyvirus, cucumovirus and begomovirus were identified for the first time, finding greater severity of symptoms with a greater number of viruses identified per sample. This research provides key results for the design of control strategies for viral diseases in passion fruit. El cultivo de maracuyá amarillo es afectado por múltiples problemas fitosanitarios. Entre los principales, están los virus de los géneros Potyvirus (soybean mosaic virus, SMV), Cucumovirus (cucumber mosaic virus, CMV), Tymovirus (passion fruit yellow mosaic virus, PFYMV) y Begomovirus (passionfruit leaf distortion virus, PLDV). Información acerca de la prevalencia e interacción entre estos virus es escasa. El objetivo del presente estudio fue verificar la prevalencia e identificar los tipos de infecciones virales mixtas de potyvirus, cucumovirus, tymovirus y begomovirus en cultivos de maracuyá en Valle del Cauca, Colombia. Muestras foliares de maracuyá con síntomas virales fueron recolectadas y se purificaron sus ácidos nucleicos. La identificación de los virus se realizó por PCR empleando cebadores universales por género viral, y cebadores específicos para los virus SMV, CMV, PFYMV y PLDV. Se analizó la incidencia de cada virus por municipio y se comparó la severidad de los síntomas con el diagnóstico realizado. En total se colectaron 66 muestras en los municipios de Toro, Dagua, Roldanillo, Bolívar, La Unión y El Cerrito. Se estableció que los virus con mayor prevalencia fueron PLDV, seguido por SMV y CMV. No se detectó la presencia de PFYMV en las muestras analizadas. Se identificó por primera vez la presencia de infecciones dobles y triples entre potyvirus, cucumovirus y begomovirus, encontrándose mayor severidad de síntomas a mayor número de virus identificado por muestra. La presente investigación provee resultados clave para el diseño de estrategias de control de enfermedades virales en maracuyá. A cultura do maracujá é afetada por múltiplos problemas fitossanitários. Entre os principais estão os vírus dos gêneros Potyvirus (Soybean mosaic virus, SMV), Cucumovirus (Cucumovirus mosaic virus, CMV), Tymovirus (Passion fruit yellow mosaic virus, PFYMV) e Begomovirus (Passion fruit leaf distortion virus, PLDV). As informações sobre a prevalência e interação entre estes vírus são escassas. O objetivo do presente estudo foi verificar a prevalência e identificar os tipos de infecções virais mistas de potyvirus, cucumovírus, tymovírus e begomovírus em cultivos de maracujá no Valle del Cauca. Foram coletadas amostras de folhas de maracujá e seus ácidos nucléicos foram purificados. A identificação do vírus foi realizada por PCR usando primers universais por gênero viral, e primers específicos para SMV, CMV, PFYMV e PLDV. A incidência de cada vírus por município foi analisada e a gravidade dos sintomas foi comparada com o diagnóstico feito. Um total de 66 amostras foi coletado nos municípios de Toro, Dagua, Roldanillo, Bolivar, La Union e El Cerrito. Os vírus mais prevalentes foram os begomovírus (45,5%), seguidos pelo PLDV (22,7%), potyvirus (21,2%), cucumovírus (19,6%), SMV (18,1%) e CMV (16,6%). O PFYMV não foi detectado nas amostras testadas. A presença de infecções duplas e triplas entre potyvirus, cucumovírus e begomovírus foi identificada pela primeira vez, e quanto maior o número de vírus identificados por amostra, maior a gravidade dos sintomas. A presente pesquisa fornece resultados-chave para o desenho de estratégias de controle de doenças virais em maracujás. Universidad de Ciencias Aplicadas y Ambientales U.D.C.A 2024-06-26 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion text/xml application/pdf https://revistas.udca.edu.co/index.php/ruadc/article/view/2364 10.31910/rudca.v27.n1.2024.2364 Revista U.D.C.A Actualidad & Divulgación Científica; Vol. 27 No. 1 (2024): Revista U.D.C.A Actualidad & Divulgación Científica. Enero-Junio Revista U.D.C.A Actualidad & Divulgación Científica; Vol. 27 Núm. 1 (2024): Revista U.D.C.A Actualidad & Divulgación Científica. Enero-Junio Revista U.D.C.A Actualidad & Divulgación Científica; v. 27 n. 1 (2024): Revista U.D.C.A Actualidad & Divulgación Científica. Enero-Junio 2619-2551 0123-4226 10.31910/rudca.v27.n1.2024 eng https://revistas.udca.edu.co/index.php/ruadc/article/view/2364/3114 https://revistas.udca.edu.co/index.php/ruadc/article/view/2364/3115 Derechos de autor 2024 Francy Jhoana Ceballos-Burgos, Jorge Iván Pérez-López, Andres Felipe Nieto-Cardenas , Diana Marcela Rivera-Toro, Jhon Albeiro Ocampo, Karina Lopez-Lopez, Juan Carlos Vaca-Vaca http://creativecommons.org/licenses/by-nc/4.0 |