Seed germination of Lotus tenuis: effect of pregerminative treatment, storage period and the role of the hard cover in the long term
It was evaluated the effect of the mechanical scarification in two moments after harvest in the seed germination response of 23 genotypes of Lotus tenuis Waldst et Kit stored under laboratory conditions without scarification and the role of the hard covers on this response. A complete random design with three factors was used [genotypes, pregerminative treatment (E=scarified; NE=non-scarified) and storage period (t1=0.25 years; t2=22.5 años)] (n=4). The percentages of cumulative germination (Gac%) and of hard seeds (Sd%) were calculated. In t2, germination speed index (IVG) was calculated. A non-parametric ANOVA (Kruskal-Wallis) and comparison of medians were applied. In t2, correlation and regression simple were analyzed. The triple interaction was significant (p≤0,01) for both parameters. Gac% had higher values in the treatment E in t1 and lower values in treatment NE in t2. The factor genotype was significant (p≤0,01) for the treatment E in t2. Sd% had higher values in the treatment NE in t1 and lower values in the treatment E regardless the storage period. The factor genotype was significant (p≤0,01) for the treatment NE in t2. The correlations Sd% vs. Gac% and IVG were significant (p≤0,01) and positives. Simple regressions SD% vs. Gac% and Sd% vs. IVG were significant (p≤0,01). This study showed that scarification and storage period affect positively and negatively, respectively, the germinative behavior of the studied genotypes, that the softening of the hard cover is variable among genotypes, and that these hard cover would be linked to the preservation of the germinative capacity in the long term.
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Facultad de Ciencias Agrarias y Forestales de la Universidad Nacional de La Plata
2020
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Lissarrague, Maria Isabel Entio, Lisandro Altamirano, Rodrigo Mujica, Mercedes |
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Lissarrague, Maria Isabel Entio, Lisandro Altamirano, Rodrigo Mujica, Mercedes Seed germination of Lotus tenuis: effect of pregerminative treatment, storage period and the role of the hard cover in the long term |
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Lissarrague, Maria Isabel Entio, Lisandro Altamirano, Rodrigo Mujica, Mercedes |
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Lissarrague, Maria Isabel |
title |
Seed germination of Lotus tenuis: effect of pregerminative treatment, storage period and the role of the hard cover in the long term |
title_short |
Seed germination of Lotus tenuis: effect of pregerminative treatment, storage period and the role of the hard cover in the long term |
title_full |
Seed germination of Lotus tenuis: effect of pregerminative treatment, storage period and the role of the hard cover in the long term |
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Seed germination of Lotus tenuis: effect of pregerminative treatment, storage period and the role of the hard cover in the long term |
title_full_unstemmed |
Seed germination of Lotus tenuis: effect of pregerminative treatment, storage period and the role of the hard cover in the long term |
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seed germination of lotus tenuis: effect of pregerminative treatment, storage period and the role of the hard cover in the long term |
description |
It was evaluated the effect of the mechanical scarification in two moments after harvest in the seed germination response of 23 genotypes of Lotus tenuis Waldst et Kit stored under laboratory conditions without scarification and the role of the hard covers on this response. A complete random design with three factors was used [genotypes, pregerminative treatment (E=scarified; NE=non-scarified) and storage period (t1=0.25 years; t2=22.5 años)] (n=4). The percentages of cumulative germination (Gac%) and of hard seeds (Sd%) were calculated. In t2, germination speed index (IVG) was calculated. A non-parametric ANOVA (Kruskal-Wallis) and comparison of medians were applied. In t2, correlation and regression simple were analyzed. The triple interaction was significant (p≤0,01) for both parameters. Gac% had higher values in the treatment E in t1 and lower values in treatment NE in t2. The factor genotype was significant (p≤0,01) for the treatment E in t2. Sd% had higher values in the treatment NE in t1 and lower values in the treatment E regardless the storage period. The factor genotype was significant (p≤0,01) for the treatment NE in t2. The correlations Sd% vs. Gac% and IVG were significant (p≤0,01) and positives. Simple regressions SD% vs. Gac% and Sd% vs. IVG were significant (p≤0,01). This study showed that scarification and storage period affect positively and negatively, respectively, the germinative behavior of the studied genotypes, that the softening of the hard cover is variable among genotypes, and that these hard cover would be linked to the preservation of the germinative capacity in the long term. |
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Facultad de Ciencias Agrarias y Forestales de la Universidad Nacional de La Plata |
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2020 |
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https://revistas.unlp.edu.ar/revagro/article/view/10198 |
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rev-revagro-ar-article-101982020-07-10T22:08:43Z Seed germination of Lotus tenuis: effect of pregerminative treatment, storage period and the role of the hard cover in the long term Germinación de semillas de Lotus tenuis: efecto del tratamiento pregerminativo, tiempo de almacenamiento y rol de la cubierta dura a largo plazo Lissarrague, Maria Isabel Entio, Lisandro Altamirano, Rodrigo Mujica, Mercedes especies forrajeras leguminosas germinacion conservacion de semillas semillas duras forages species legumes germination seed conservation hard seed It was evaluated the effect of the mechanical scarification in two moments after harvest in the seed germination response of 23 genotypes of Lotus tenuis Waldst et Kit stored under laboratory conditions without scarification and the role of the hard covers on this response. A complete random design with three factors was used [genotypes, pregerminative treatment (E=scarified; NE=non-scarified) and storage period (t1=0.25 years; t2=22.5 años)] (n=4). The percentages of cumulative germination (Gac%) and of hard seeds (Sd%) were calculated. In t2, germination speed index (IVG) was calculated. A non-parametric ANOVA (Kruskal-Wallis) and comparison of medians were applied. In t2, correlation and regression simple were analyzed. The triple interaction was significant (p≤0,01) for both parameters. Gac% had higher values in the treatment E in t1 and lower values in treatment NE in t2. The factor genotype was significant (p≤0,01) for the treatment E in t2. Sd% had higher values in the treatment NE in t1 and lower values in the treatment E regardless the storage period. The factor genotype was significant (p≤0,01) for the treatment NE in t2. The correlations Sd% vs. Gac% and IVG were significant (p≤0,01) and positives. Simple regressions SD% vs. Gac% and Sd% vs. IVG were significant (p≤0,01). This study showed that scarification and storage period affect positively and negatively, respectively, the germinative behavior of the studied genotypes, that the softening of the hard cover is variable among genotypes, and that these hard cover would be linked to the preservation of the germinative capacity in the long term. Se evaluó el efecto de la escarificación mecánica en dos momentos luego de la cosecha sobre la germinación de semillas de 23 genotipos de Lotus tenuis Waldst et Kit almacenadas en condiciones de laboratorio sin escarificar y el rol de las cubiertas duras sobre dicha respuesta a largo plazo. Se usó un DCA con tres factores [genotipos, tratamiento pregerminativo (E=escarificado; NE=ausencia de escarificado) y tiempo de almacenamiento (t1=0,25 años; t2=22,5 años)] (n=4). Se calculó el porcentaje de germinación acumulada (Gac%) y de semillas duras (Sd%). Para t2 se calculó el índice de velocidad germinativa (IVG). Se aplicó ANOVA no paramétrico (Kruskal-Wallis) y comparación de medianas. Para t2 se analizaron correlación y regresión simple. La interacción triple resultó significativa (p ≤0,01) para ambos parámetros. Gac% tuvo valores mayores en el tratamiento E en t1 y menores en NE en t2. El factor genotipo resultó significativo (p≤0,01) para el E en t2. Sd% tuvo valores mayores en el tratamiento NE en t1 y menores en E independientemente del tiempo de almacenamiento. El factor genotipo resultó significativo (p≤0,01) para NE en t2. La correlación Sd% vs. Gac% e IVG resultó significativa (p≤0,01) y positiva. Las regresiones simples Sd% vs. Gac% e IVG resultaron significativas (p≤0,01). El estudio demostró que la escarificación y el tiempo de almacenamiento afectan de manera positiva y negativa, respectivamente, al comportamiento germinativo de los genotipos estudiados, que el ablandamiento de la cubierta dura es variable entre genotipos y que dicha cubierta estaría vinculada con la conservación de la capacidad germinativa en el largo plazo. Facultad de Ciencias Agrarias y Forestales de la Universidad Nacional de La Plata 2020-07-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo evaluado por pares Peer reviewed article application/pdf https://revistas.unlp.edu.ar/revagro/article/view/10198 10.24215/16699513e043 Journal of the Agronomy College; Vol. 119 No. 1 (2020): Revista de la Facultad de Agronomia; 043 Revista de la Facultad de Agronomía; Vol. 119 Núm. 1 (2020): Revista de la Facultad de Agronomia; 043 Journal de la Faculté d'Agronomie; Vol. 119 No. 1 (2020): Revista de la Facultad de Agronomia; 043 Revista da Faculdade de Agronomia; v. 119 n. 1 (2020): Revista de la Facultad de Agronomia; 043 1669-9513 0041-8676 spa https://revistas.unlp.edu.ar/revagro/article/view/10198/8993 |