Anthelmintic evaluation of four fodder tree extracts against the nematode Haemonchus contortus under in vitro conditions
The objective was to evaluate the nematocidal effect of four hydroalcoholic extracts (HAE) of Brosimum alicastrum (HAE-Ba), Guazuma ulmifolia (HAE-Gu), Erythrina americana (HAE-Ea) and Leucaena leucocephala (HAE-Ll) against Haemonchus contortus. The tests of egg hatching inhibition (EHI) and larval (infective larvae) mortality were used. The treatments were HAEs at concentrations of 6.25-50 mg/mL for EHI and 25-100 mg/mL for larval mortality, ivermectin (5 mg/mL, positive control) and distilled water (negative control). Data were analyzed using an ANOVA and treatments with a concentration-dependent effect were subjected to a regression analysis to determine lethal concentrations (LC50 and LC90). In addition, a phytochemical analysis was performed on the extracts to identify the presence of the main secondary metabolites. The best ovicidal and larvicidal activity was observed in HAE-Gu with 96.78 % EHI at 6.25 mg/mL and 57.2 % larval mortality at 75 mg/mL. Followed by HAE-Ba showing 90 % EHI at 6.25 mg/mL and 58.0 % larval mortality at 75 mg/mL. The LC50 and LC90 of HAE-Gu on EHI were 2.7 and 4.4 mg/mL, respectively. While the LCs of this same extract on larvae were LC50= 64 and LC90= 125 mg/mL. The phytochemical analysis indicates that all extracts contain tannins, coumarins, flavonoids and terpenes. The fodder species G. ulmifolia and E. americana could be candidate plants for the control of H. contortus.
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Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2023
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Santiago-Figueroa, Itzel Lara-Bueno, Alejandro González-Garduño, Roberto Mendoza-de Gives, Pedro Delgado-Núñez, Edgar Jesús Maldonado-Simán, Ema de Jesús Garedaghi, Yagoob Olmedo-Juárez, Agustín |
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Santiago-Figueroa, Itzel Lara-Bueno, Alejandro González-Garduño, Roberto Mendoza-de Gives, Pedro Delgado-Núñez, Edgar Jesús Maldonado-Simán, Ema de Jesús Garedaghi, Yagoob Olmedo-Juárez, Agustín Anthelmintic evaluation of four fodder tree extracts against the nematode Haemonchus contortus under in vitro conditions |
| author_facet |
Santiago-Figueroa, Itzel Lara-Bueno, Alejandro González-Garduño, Roberto Mendoza-de Gives, Pedro Delgado-Núñez, Edgar Jesús Maldonado-Simán, Ema de Jesús Garedaghi, Yagoob Olmedo-Juárez, Agustín |
| author_sort |
Santiago-Figueroa, Itzel |
| title |
Anthelmintic evaluation of four fodder tree extracts against the nematode Haemonchus contortus under in vitro conditions |
| title_short |
Anthelmintic evaluation of four fodder tree extracts against the nematode Haemonchus contortus under in vitro conditions |
| title_full |
Anthelmintic evaluation of four fodder tree extracts against the nematode Haemonchus contortus under in vitro conditions |
| title_fullStr |
Anthelmintic evaluation of four fodder tree extracts against the nematode Haemonchus contortus under in vitro conditions |
| title_full_unstemmed |
Anthelmintic evaluation of four fodder tree extracts against the nematode Haemonchus contortus under in vitro conditions |
| title_sort |
anthelmintic evaluation of four fodder tree extracts against the nematode haemonchus contortus under in vitro conditions |
| description |
The objective was to evaluate the nematocidal effect of four hydroalcoholic extracts (HAE) of Brosimum alicastrum (HAE-Ba), Guazuma ulmifolia (HAE-Gu), Erythrina americana (HAE-Ea) and Leucaena leucocephala (HAE-Ll) against Haemonchus contortus. The tests of egg hatching inhibition (EHI) and larval (infective larvae) mortality were used. The treatments were HAEs at concentrations of 6.25-50 mg/mL for EHI and 25-100 mg/mL for larval mortality, ivermectin (5 mg/mL, positive control) and distilled water (negative control). Data were analyzed using an ANOVA and treatments with a concentration-dependent effect were subjected to a regression analysis to determine lethal concentrations (LC50 and LC90). In addition, a phytochemical analysis was performed on the extracts to identify the presence of the main secondary metabolites. The best ovicidal and larvicidal activity was observed in HAE-Gu with 96.78 % EHI at 6.25 mg/mL and 57.2 % larval mortality at 75 mg/mL. Followed by HAE-Ba showing 90 % EHI at 6.25 mg/mL and 58.0 % larval mortality at 75 mg/mL. The LC50 and LC90 of HAE-Gu on EHI were 2.7 and 4.4 mg/mL, respectively. While the LCs of this same extract on larvae were LC50= 64 and LC90= 125 mg/mL. The phytochemical analysis indicates that all extracts contain tannins, coumarins, flavonoids and terpenes. The fodder species G. ulmifolia and E. americana could be candidate plants for the control of H. contortus. |
| publisher |
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias |
| publishDate |
2023 |
| url |
https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/6339 |
| work_keys_str_mv |
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rev-remexcp-article63392023-10-02T17:58:37Z Anthelmintic evaluation of four fodder tree extracts against the nematode Haemonchus contortus under in vitro conditions Evaluación antihelmíntica de cuatro extractos de árboles forrajeros contra el nematodo Haemonchus contortus bajo condiciones in vitro Santiago-Figueroa, Itzel Lara-Bueno, Alejandro González-Garduño, Roberto Mendoza-de Gives, Pedro Delgado-Núñez, Edgar Jesús Maldonado-Simán, Ema de Jesús Garedaghi, Yagoob Olmedo-Juárez, Agustín Fodder trees; Secondary metabolites; Haemonchus contortus; Larval mortality; Egg hatching inhibition Arboles forrajeros; Metabolitos secundarios; Haemonchus contortus Mortalidad de larvas; Inhibición de la eclosión de huevos The objective was to evaluate the nematocidal effect of four hydroalcoholic extracts (HAE) of Brosimum alicastrum (HAE-Ba), Guazuma ulmifolia (HAE-Gu), Erythrina americana (HAE-Ea) and Leucaena leucocephala (HAE-Ll) against Haemonchus contortus. The tests of egg hatching inhibition (EHI) and larval (infective larvae) mortality were used. The treatments were HAEs at concentrations of 6.25-50 mg/mL for EHI and 25-100 mg/mL for larval mortality, ivermectin (5 mg/mL, positive control) and distilled water (negative control). Data were analyzed using an ANOVA and treatments with a concentration-dependent effect were subjected to a regression analysis to determine lethal concentrations (LC50 and LC90). In addition, a phytochemical analysis was performed on the extracts to identify the presence of the main secondary metabolites. The best ovicidal and larvicidal activity was observed in HAE-Gu with 96.78 % EHI at 6.25 mg/mL and 57.2 % larval mortality at 75 mg/mL. Followed by HAE-Ba showing 90 % EHI at 6.25 mg/mL and 58.0 % larval mortality at 75 mg/mL. The LC50 and LC90 of HAE-Gu on EHI were 2.7 and 4.4 mg/mL, respectively. While the LCs of this same extract on larvae were LC50= 64 and LC90= 125 mg/mL. The phytochemical analysis indicates that all extracts contain tannins, coumarins, flavonoids and terpenes. The fodder species G. ulmifolia and E. americana could be candidate plants for the control of H. contortus. El objetivo del presente estudio fue evaluar el efecto nematicida de cuatro extractos hidroalcohólicos (EHA) de Brosimum alicastrum (EHA-Ba), Guazuma ulmifolia (EHA-Gu), Erythrina americana (EHA-Ea) y Leucaena leucocephala (EHA-Ll) contra Haemonchus contortus. Se usaron las pruebas de inhibición de la eclosión de huevos (IEH) y mortalidad larval (larvas infectantes). Los tratamientos fueron los EHA´s a concentraciones de 6.25-50 mg/ml para IEH y de 25-100 mg/mlpara mortalidad larval, ivermectina (5 mg/ml, control positivo) y agua destilada (control negativo). Los datos se analizaron mediante un ANOVA y los tratamientos con efecto dependiente a la concentración se sometieron a un análisis de regresión para determinar las concentraciones letales (CL50 y CL90). Además, se realizó un análisis fitoquímico a los extractos para identificar la presencia de los principales metabolitos secundarios. La mejor actividad ovicida y larvicida fue observada en EHA-Gu con un 96.78 % de IEH a 6.25 mg/ml y 57.2 % de mortalidad larval a 75 mg/ml. Seguido de EHA-Ba mostrando 90 % IEH a 6.25 mg/mly un 58.0 % de mortalidad larval a 75 mg/ml. Las CL50 y CL90 del EHA-Gu sobre la IEH fueron 2.7 y 4.4 mg/ml, respectivamente. Mientras que las CL´s de este mismo extracto sobre larvas fue de CL50=64 y CL90=125 mg/ml. El análisis fitoquímico indica que todos los extractos contienen taninos, cumarinas, flavonoides y terpenos. Las especies forrajeras G. ulmifolia y E. americana podrían ser plantas candidatas para el control de H. contortus. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias 2023-10-02 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/pdf text/html https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/6339 10.22319/rmcp.v14i4.6339 Revista Mexicana de Ciencias Pecuarias; Vol. 14, Núm. 4 (2023): Octubre-Diciembre; 855-873 Revista Mexicana de Ciencias Pecuarias; Vol. 14, Núm. 4 (2023): Octubre-Diciembre; 855-873 2448-6698 2007-1124 spa eng https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/6339/5187 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/6339/5188 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/6339/5230 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/6339/2918 https://creativecommons.org/licenses/by-nc-sa/4.0 |