Prevalence of the qnrB, qnrA and blaTEM genes in temperate bacteriophages of Escherichia coli isolated from wastewater and sewer water from slaughterhouses in the State of Mexico
Antibiotic resistance genes (ARG) have been described mainly in bacteria, but are known to occur in temperate phages. Prevalence of the qnrB, qnrA and blaTEM genes was identified in Escherichia coli strains and temperate phages by lytic cycle induction. From a total of 48 samples collected from drinking water, wastewater and sewer water in slaughterhouses in the State of Mexico, Mexico, 37 contained E. coli isolates. Resistance was highest to tetracycline (32/37; 86.4 %), followed by trimethoprim-sulfamethoxazole (19/37; 51.3 %) and ampicillin and nalidixic acid (18/37; 48.6 %). Prevalence of the blaTEM gene was 37.8 % in the bacterial isolates and 3.5 % in the phage isolates. The bacterial isolates contained 8.1 % qnrA and 29.7 % qnrB genes, while the phage isolates contained 2.7 and 24.3 %, respectively. Presence of ARG in the bacterial isolates was linked to phage DNA, highlighting the significant role it plays in the spread of ARG in the studied slaughterhouses. Understanding the mechanisms of antimicrobial resistance will contribute to developing effective control measures.
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Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2021
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rev-remexcp-article53782023-04-25T22:02:56Z Prevalence of the qnrB, qnrA and blaTEM genes in temperate bacteriophages of Escherichia coli isolated from wastewater and sewer water from slaughterhouses in the State of Mexico Prevalencia de genes qnrB, qnrA y blaTEM en bacteriófagos atemperados de Escherichia coli aislados en agua residual y alcantarillas de rastros del Estado de México Talavera-González, Juan Martín Acosta-Dibarrat, Jorge Reyes-Rodríguez, Nydia Edith Salgado-Miranda, Celene Talavera-Rojas, Martín Bacteriophages; Escherichia coli; Genes; Resistance Bacteriófagos; Escherichia coli; Genes; Resistencia Antibiotic resistance genes (ARG) have been described mainly in bacteria, but are known to occur in temperate phages. Prevalence of the qnrB, qnrA and blaTEM genes was identified in Escherichia coli strains and temperate phages by lytic cycle induction. From a total of 48 samples collected from drinking water, wastewater and sewer water in slaughterhouses in the State of Mexico, Mexico, 37 contained E. coli isolates. Resistance was highest to tetracycline (32/37; 86.4 %), followed by trimethoprim-sulfamethoxazole (19/37; 51.3 %) and ampicillin and nalidixic acid (18/37; 48.6 %). Prevalence of the blaTEM gene was 37.8 % in the bacterial isolates and 3.5 % in the phage isolates. The bacterial isolates contained 8.1 % qnrA and 29.7 % qnrB genes, while the phage isolates contained 2.7 and 24.3 %, respectively. Presence of ARG in the bacterial isolates was linked to phage DNA, highlighting the significant role it plays in the spread of ARG in the studied slaughterhouses. Understanding the mechanisms of antimicrobial resistance will contribute to developing effective control measures. Los genes de resistencia a los antibióticos (ARG) han sido descritos principalmente en bacterias; sin embargo, en fagos atemperados los estudios han sido escasos. En este estudio se determinó la prevalencia de los genes qnrB, qnrA y blaTEM en cepas de Escherichia coli y en fagos atemperados obtenidos por inducción del ciclo lítico en dichos aislamientos. Se recolectaron 48 muestras de agua potable, agua residual y alcantarillado en rastros del Estado de México obteniendo 37 aislamientos de E. coli. La mayor resistencia fue para tetraciclina 32/37 (86.4 %), seguido de trimetoprim-sulfametoxasol 19/37 (51.3 %) y por último ampicilina y ácido nalidíxico con el mismo número de aislamientos 18/37 (48.6 %). La prevalencia del gen blaTEM en aislamientos bacterianos fue 37.8 %, mientras que en los aislamientos fágicos fue 3.5 %. Los genes qnrA y qnrB fueron encontrado s en 8.1 % y 29.7 % respectivamente en aislamientos bacterianos, mientras que en los aislamientos fágicos fueron obtenidos 2.7 y 24.3 % respectivamente. Los resultados muestran que los ARG presentes en aislamientos bacterianos se relacionan en el ADN fágico, lo que indica el papel significativo en la propagación de ARG en los rastros estudiados. Comprender los mecanismos de resistencia a antimicrobianos, permitirá establecer medidas efectivas para disminuir este fenómeno. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias 2021-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/pdf text/html https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5378 10.22319/rmcp.v12i1.5378 Revista Mexicana de Ciencias Pecuarias; Vol. 12, Núm. 1 (2021): Enero-Marzo; 298-305 Revista Mexicana de Ciencias Pecuarias; Vol. 12, Núm. 1 (2021): Enero-Marzo; 298-305 2448-6698 2007-1124 spa eng https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5378/4518 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5378/4519 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5378/5160 http://creativecommons.org/licenses/by-nc-sa/4.0 |
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Talavera-González, Juan Martín Acosta-Dibarrat, Jorge Reyes-Rodríguez, Nydia Edith Salgado-Miranda, Celene Talavera-Rojas, Martín |
spellingShingle |
Talavera-González, Juan Martín Acosta-Dibarrat, Jorge Reyes-Rodríguez, Nydia Edith Salgado-Miranda, Celene Talavera-Rojas, Martín Prevalence of the qnrB, qnrA and blaTEM genes in temperate bacteriophages of Escherichia coli isolated from wastewater and sewer water from slaughterhouses in the State of Mexico |
author_facet |
Talavera-González, Juan Martín Acosta-Dibarrat, Jorge Reyes-Rodríguez, Nydia Edith Salgado-Miranda, Celene Talavera-Rojas, Martín |
author_sort |
Talavera-González, Juan Martín |
title |
Prevalence of the qnrB, qnrA and blaTEM genes in temperate bacteriophages of Escherichia coli isolated from wastewater and sewer water from slaughterhouses in the State of Mexico |
title_short |
Prevalence of the qnrB, qnrA and blaTEM genes in temperate bacteriophages of Escherichia coli isolated from wastewater and sewer water from slaughterhouses in the State of Mexico |
title_full |
Prevalence of the qnrB, qnrA and blaTEM genes in temperate bacteriophages of Escherichia coli isolated from wastewater and sewer water from slaughterhouses in the State of Mexico |
title_fullStr |
Prevalence of the qnrB, qnrA and blaTEM genes in temperate bacteriophages of Escherichia coli isolated from wastewater and sewer water from slaughterhouses in the State of Mexico |
title_full_unstemmed |
Prevalence of the qnrB, qnrA and blaTEM genes in temperate bacteriophages of Escherichia coli isolated from wastewater and sewer water from slaughterhouses in the State of Mexico |
title_sort |
prevalence of the qnrb, qnra and blatem genes in temperate bacteriophages of escherichia coli isolated from wastewater and sewer water from slaughterhouses in the state of mexico |
description |
Antibiotic resistance genes (ARG) have been described mainly in bacteria, but are known to occur in temperate phages. Prevalence of the qnrB, qnrA and blaTEM genes was identified in Escherichia coli strains and temperate phages by lytic cycle induction. From a total of 48 samples collected from drinking water, wastewater and sewer water in slaughterhouses in the State of Mexico, Mexico, 37 contained E. coli isolates. Resistance was highest to tetracycline (32/37; 86.4 %), followed by trimethoprim-sulfamethoxazole (19/37; 51.3 %) and ampicillin and nalidixic acid (18/37; 48.6 %). Prevalence of the blaTEM gene was 37.8 % in the bacterial isolates and 3.5 % in the phage isolates. The bacterial isolates contained 8.1 % qnrA and 29.7 % qnrB genes, while the phage isolates contained 2.7 and 24.3 %, respectively. Presence of ARG in the bacterial isolates was linked to phage DNA, highlighting the significant role it plays in the spread of ARG in the studied slaughterhouses. Understanding the mechanisms of antimicrobial resistance will contribute to developing effective control measures. |
publisher |
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias |
publishDate |
2021 |
url |
https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5378 |
work_keys_str_mv |
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