Frequency of M. hyopneumoniae, M. hyorhinis and M. hyosynoviae in nasal and lung samples from pigs with symptoms of porcine enzootic pneumonia
M. hyopneumoniae, M. hyorhinis and M. hyosynoviae are genetically related species of the genus Mycoplasma that affect pig production. The objective of this work was the isolation and identification by PCR of M. hyopneumoniae, M. hyorhinis and M. hyosynoviae from nasal swabs and lung samples of pigs from different regions of Mexico in order to determine the frequency of these species and to evaluate PCR as a diagnostic tool for PEP. Pigs aged 4 to 8 weeks with clinical diagnosis of PEP were included. Lung samples and nasal swabs were obtained for the isolation of the Mycoplasma in liquid Friis medium and identified by species-specific PCR based on the 16S rRNA subunit. Isolation was achieved in 37.11 % (36/97) of the samples. The three Mycoplasma species were identified in lung and nasal swab samples. Mycoplasma co-infection was identified in 27.77 % (10/36). The bacterial genera associated with Mycoplasma infections were E. coli, Bordetella, Enterobacter, SCN, Corynebacterium, Pasteurella, Streptococcus, Shigella and Klebsiella. Mixed infection was present in 26 nasal swabs (45.61 %) and absent in the lungs. It was concluded that the frequency of Mycoplasma on production farms was higher than expected (40.27 %). It was also identified other Mycoplasma species involved in the development of PEP. Therefore, surveillance through isolation and molecular techniques can be of great help to breeding stock providers, as well as for removing Mycoplasma from pig farms.
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Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
2020
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Miranda Morales, Rosa Elena Rojas Trejo, Veronica López-Cerino, Luis Enrique Carrillo Casas, Erika Margarita Sarmiento Silva, Rosa Elena Trujillo Ortega, María Elena Beltrán Figueroa, Rolando Trigo Tavera, Francisco José |
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Miranda Morales, Rosa Elena Rojas Trejo, Veronica López-Cerino, Luis Enrique Carrillo Casas, Erika Margarita Sarmiento Silva, Rosa Elena Trujillo Ortega, María Elena Beltrán Figueroa, Rolando Trigo Tavera, Francisco José Frequency of M. hyopneumoniae, M. hyorhinis and M. hyosynoviae in nasal and lung samples from pigs with symptoms of porcine enzootic pneumonia |
| author_facet |
Miranda Morales, Rosa Elena Rojas Trejo, Veronica López-Cerino, Luis Enrique Carrillo Casas, Erika Margarita Sarmiento Silva, Rosa Elena Trujillo Ortega, María Elena Beltrán Figueroa, Rolando Trigo Tavera, Francisco José |
| author_sort |
Miranda Morales, Rosa Elena |
| title |
Frequency of M. hyopneumoniae, M. hyorhinis and M. hyosynoviae in nasal and lung samples from pigs with symptoms of porcine enzootic pneumonia |
| title_short |
Frequency of M. hyopneumoniae, M. hyorhinis and M. hyosynoviae in nasal and lung samples from pigs with symptoms of porcine enzootic pneumonia |
| title_full |
Frequency of M. hyopneumoniae, M. hyorhinis and M. hyosynoviae in nasal and lung samples from pigs with symptoms of porcine enzootic pneumonia |
| title_fullStr |
Frequency of M. hyopneumoniae, M. hyorhinis and M. hyosynoviae in nasal and lung samples from pigs with symptoms of porcine enzootic pneumonia |
| title_full_unstemmed |
Frequency of M. hyopneumoniae, M. hyorhinis and M. hyosynoviae in nasal and lung samples from pigs with symptoms of porcine enzootic pneumonia |
| title_sort |
frequency of m. hyopneumoniae, m. hyorhinis and m. hyosynoviae in nasal and lung samples from pigs with symptoms of porcine enzootic pneumonia |
| description |
M. hyopneumoniae, M. hyorhinis and M. hyosynoviae are genetically related species of the genus Mycoplasma that affect pig production. The objective of this work was the isolation and identification by PCR of M. hyopneumoniae, M. hyorhinis and M. hyosynoviae from nasal swabs and lung samples of pigs from different regions of Mexico in order to determine the frequency of these species and to evaluate PCR as a diagnostic tool for PEP. Pigs aged 4 to 8 weeks with clinical diagnosis of PEP were included. Lung samples and nasal swabs were obtained for the isolation of the Mycoplasma in liquid Friis medium and identified by species-specific PCR based on the 16S rRNA subunit. Isolation was achieved in 37.11 % (36/97) of the samples. The three Mycoplasma species were identified in lung and nasal swab samples. Mycoplasma co-infection was identified in 27.77 % (10/36). The bacterial genera associated with Mycoplasma infections were E. coli, Bordetella, Enterobacter, SCN, Corynebacterium, Pasteurella, Streptococcus, Shigella and Klebsiella. Mixed infection was present in 26 nasal swabs (45.61 %) and absent in the lungs. It was concluded that the frequency of Mycoplasma on production farms was higher than expected (40.27 %). It was also identified other Mycoplasma species involved in the development of PEP. Therefore, surveillance through isolation and molecular techniques can be of great help to breeding stock providers, as well as for removing Mycoplasma from pig farms. |
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Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias |
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2020 |
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https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5124 |
| work_keys_str_mv |
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rev-remexcp-article51242023-02-14T16:41:25Z Frequency of M. hyopneumoniae, M. hyorhinis and M. hyosynoviae in nasal and lung samples from pigs with symptoms of porcine enzootic pneumonia Frecuencia de M. hyopneumoniae, M. hyorhinis y M. hyosynoviae en muestras nasales y de pulmón de cerdos con síntomas de neumonía enzoótica porcina Miranda Morales, Rosa Elena Rojas Trejo, Veronica López-Cerino, Luis Enrique Carrillo Casas, Erika Margarita Sarmiento Silva, Rosa Elena Trujillo Ortega, María Elena Beltrán Figueroa, Rolando Trigo Tavera, Francisco José Mycoplasmosis; M. hyopneumoniae; M. hyorhinis; M. hyosynoviae; Porcine enzootic pneumonia Micoplasmosis; M. hyopneumoniae; M. hyorhinis; M. hyosynoviae; Neumonía enzoótica porcina M. hyopneumoniae, M. hyorhinis and M. hyosynoviae are genetically related species of the genus Mycoplasma that affect pig production. The objective of this work was the isolation and identification by PCR of M. hyopneumoniae, M. hyorhinis and M. hyosynoviae from nasal swabs and lung samples of pigs from different regions of Mexico in order to determine the frequency of these species and to evaluate PCR as a diagnostic tool for PEP. Pigs aged 4 to 8 weeks with clinical diagnosis of PEP were included. Lung samples and nasal swabs were obtained for the isolation of the Mycoplasma in liquid Friis medium and identified by species-specific PCR based on the 16S rRNA subunit. Isolation was achieved in 37.11 % (36/97) of the samples. The three Mycoplasma species were identified in lung and nasal swab samples. Mycoplasma co-infection was identified in 27.77 % (10/36). The bacterial genera associated with Mycoplasma infections were E. coli, Bordetella, Enterobacter, SCN, Corynebacterium, Pasteurella, Streptococcus, Shigella and Klebsiella. Mixed infection was present in 26 nasal swabs (45.61 %) and absent in the lungs. It was concluded that the frequency of Mycoplasma on production farms was higher than expected (40.27 %). It was also identified other Mycoplasma species involved in the development of PEP. Therefore, surveillance through isolation and molecular techniques can be of great help to breeding stock providers, as well as for removing Mycoplasma from pig farms. M. hyopneumoniae, M. hyorhinis y M. hyopsynoviae son especies genéticamente relacionadas del género Mycoplasma que afectan la producción porcina. El objetivo de este trabajo fue el aislamiento e identificación por PCR de M. hyopneumoniae, M. hyorhinis y M. hyosynoviae a partir de hisopos nasales y muestras de pulmón de cerdos de diferentes regiones mexicanas para determinar la frecuencia de estas especies y evaluar la PCR como herramienta diagnóstica para NEP. Se incluyeron cerdos de 4 a 8 semanas con diagnóstico clínico de NEP. Se obtuvieron muestras de pulmón e hisopos nasales para el aislamiento de Mycoplasma en medio Friis líquido y se identificaron mediante la PCR especie-específica basada en la subunidad 16S rRNA. El aislamiento se logró en 37.11 % (36/97) muestras. Las tres especies de Mycoplasma se identificaron en muestras de pulmón e hisopos nasales. La co-infección por Mycoplasma se identificó en el 27.77 % (10/36). Los géneros bacterianos asociados a las infecciones por Mycoplasma fueron E. coli, Bordetella, Enterobacter, SCN, Corynebacterium, Pasteurella, Streptococcus, Shigella y Kebsiella. La infección mixta estuvo presente en 26 hisopos nasales (45.61 %) y ausente en pulmones. Se concluyó que la frecuencia de Mycoplasma en las fincas de producción fue mayor a la esperada (40.27 %). También se identificaron otras especies de Mycoplasma involucradas en el desarrollo de la NEP. Por lo tanto, la vigilancia asistida por el aislamiento y las técnicas moleculares pueden ser de gran ayuda para la eliminación de Mycoplasma de las explotaciones porcinas y para los proveedores de pie de cría. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias Universidad Nacional Autónoma de México 2020-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/pdf text/html https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5124 10.22319/rmcp.v11i4.5124 Revista Mexicana de Ciencias Pecuarias; Vol. 11, Núm. 4 (2020): Octubre-Diciembre; 946-960 Revista Mexicana de Ciencias Pecuarias; Vol. 11, Núm. 4 (2020): Octubre-Diciembre; 946-960 2448-6698 2007-1124 spa eng https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5124/4439 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5124/4438 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/5124/5099 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/5124/1206 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/5124/1207 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/5124/1208 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/5124/1209 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/5124/1210 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/5124/1211 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/5124/1212 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/5124/1519 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/downloadSuppFile/5124/1520 http://creativecommons.org/licenses/by-nc-sa/4.0 |