In vitro production of porcine embryos with use of chemically semi-defined culture media system

In vitro production of porcine embryos with use of chemically semi-defined culture media system

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The objective of this study was to determine the effect of a semi-defined culture media system developed in our laboratory, named Pigs Media System (PMS) on the in vitro production of porcine embryos. In a first assay, the cummulus-oocytes complexes (COCs) were matured, fertilized and cultured for embryo development in PMS supplemented with bovine serum albumin (BSA), and in North Carolina State University-23 (NCSU-23) supplemented with fluid follicular, until blastocysts evaluation. In the assay 2, maturation and culture were performed in PMS using BSA or polyvinyl alcohol (PVA) in a 2 × 2 factorial arrangement (PMS-BSA/BSA, PMS-BSA/PVA, PMS-PVA/PVA, PMS-PVA/BSA). The PMS had a positive effect on the total cell number (58.04) and the decrease of the total lipids (49.4%) regarding the NCSU-23 medium (37.98 and 59.2% respectively; p<0.05). The percentage of monospermic fertilization was significantly lower (42.3%; p<0.05) when oocytes were matured with PMS-BSA than in PMS-PVA (52.6%). The supplementation of BSA in the PMS for embryo culture, increased the blastocyst development, the cell number of blastocysts and decreased the content of total lipids (36.8%, 46.9 and 49.6% respectively; p<0.05), in comparison with the supplementation of PVA in the PMS for embryo culture. These results suggest that the semi-defined culture media system developed by the National Genetic Resources Center (CNRG), have proved favorable effects on the total cell number and the decrease of total lipids of porcine blastocysts in vitro produced. 

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Main Authors: Urbán Duarte, David, Álvarez Gallardo, Horacio, Pérez Reynozo, Sandra, De la Torre Sánchez, José Fernando
Format: Digital revista
Language:eng
spa
Published: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias 2020
Online Access:https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/4757
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id rev-remexcp-article4757
record_format ojs
institution INIFAP
collection OJS
country México
countrycode MX
component Revista
access En linea
databasecode rev-remexcp
tag revista
region America del Norte
libraryname Biblioteca INIFAP
language eng
spa
format Digital
author Urbán Duarte, David
Álvarez Gallardo, Horacio
Pérez Reynozo, Sandra
De la Torre Sánchez, José Fernando
spellingShingle Urbán Duarte, David
Álvarez Gallardo, Horacio
Pérez Reynozo, Sandra
De la Torre Sánchez, José Fernando
In vitro production of porcine embryos with use of chemically semi-defined culture media system
author_facet Urbán Duarte, David
Álvarez Gallardo, Horacio
Pérez Reynozo, Sandra
De la Torre Sánchez, José Fernando
author_sort Urbán Duarte, David
title In vitro production of porcine embryos with use of chemically semi-defined culture media system
title_short In vitro production of porcine embryos with use of chemically semi-defined culture media system
title_full In vitro production of porcine embryos with use of chemically semi-defined culture media system
title_fullStr In vitro production of porcine embryos with use of chemically semi-defined culture media system
title_full_unstemmed In vitro production of porcine embryos with use of chemically semi-defined culture media system
title_sort in vitro production of porcine embryos with use of chemically semi-defined culture media system
description The objective of this study was to determine the effect of a semi-defined culture media system developed in our laboratory, named Pigs Media System (PMS) on the in vitro production of porcine embryos. In a first assay, the cummulus-oocytes complexes (COCs) were matured, fertilized and cultured for embryo development in PMS supplemented with bovine serum albumin (BSA), and in North Carolina State University-23 (NCSU-23) supplemented with fluid follicular, until blastocysts evaluation. In the assay 2, maturation and culture were performed in PMS using BSA or polyvinyl alcohol (PVA) in a 2 × 2 factorial arrangement (PMS-BSA/BSA, PMS-BSA/PVA, PMS-PVA/PVA, PMS-PVA/BSA). The PMS had a positive effect on the total cell number (58.04) and the decrease of the total lipids (49.4%) regarding the NCSU-23 medium (37.98 and 59.2% respectively; p<0.05). The percentage of monospermic fertilization was significantly lower (42.3%; p<0.05) when oocytes were matured with PMS-BSA than in PMS-PVA (52.6%). The supplementation of BSA in the PMS for embryo culture, increased the blastocyst development, the cell number of blastocysts and decreased the content of total lipids (36.8%, 46.9 and 49.6% respectively; p<0.05), in comparison with the supplementation of PVA in the PMS for embryo culture. These results suggest that the semi-defined culture media system developed by the National Genetic Resources Center (CNRG), have proved favorable effects on the total cell number and the decrease of total lipids of porcine blastocysts in vitro produced. 
publisher Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
publishDate 2020
url https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/4757
work_keys_str_mv AT urbanduartedavid invitroproductionofporcineembryoswithuseofchemicallysemidefinedculturemediasystem
AT alvarezgallardohoracio invitroproductionofporcineembryoswithuseofchemicallysemidefinedculturemediasystem
AT perezreynozosandra invitroproductionofporcineembryoswithuseofchemicallysemidefinedculturemediasystem
AT delatorresanchezjosefernando invitroproductionofporcineembryoswithuseofchemicallysemidefinedculturemediasystem
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spelling rev-remexcp-article47572022-06-17T15:43:25Z In vitro production of porcine embryos with use of chemically semi-defined culture media system In vitro production of porcine embryos with use of chemically semi-defined culture media system Urbán Duarte, David Álvarez Gallardo, Horacio Pérez Reynozo, Sandra De la Torre Sánchez, José Fernando culture media; pigs; embryo; in vitro production culture media; pigs; embryo; in vitro production The objective of this study was to determine the effect of a semi-defined culture media system developed in our laboratory, named Pigs Media System (PMS) on the in vitro production of porcine embryos. In a first assay, the cummulus-oocytes complexes (COCs) were matured, fertilized and cultured for embryo development in PMS supplemented with bovine serum albumin (BSA), and in North Carolina State University-23 (NCSU-23) supplemented with fluid follicular, until blastocysts evaluation. In the assay 2, maturation and culture were performed in PMS using BSA or polyvinyl alcohol (PVA) in a 2 × 2 factorial arrangement (PMS-BSA/BSA, PMS-BSA/PVA, PMS-PVA/PVA, PMS-PVA/BSA). The PMS had a positive effect on the total cell number (58.04) and the decrease of the total lipids (49.4%) regarding the NCSU-23 medium (37.98 and 59.2% respectively; p<0.05). The percentage of monospermic fertilization was significantly lower (42.3%; p<0.05) when oocytes were matured with PMS-BSA than in PMS-PVA (52.6%). The supplementation of BSA in the PMS for embryo culture, increased the blastocyst development, the cell number of blastocysts and decreased the content of total lipids (36.8%, 46.9 and 49.6% respectively; p<0.05), in comparison with the supplementation of PVA in the PMS for embryo culture. These results suggest that the semi-defined culture media system developed by the National Genetic Resources Center (CNRG), have proved favorable effects on the total cell number and the decrease of total lipids of porcine blastocysts in vitro produced.  The objective of this study was to determine the effect of a semi-defined culture media system developed in our laboratory, named Pigs Media System (PMS) on the in vitro production of porcine embryos. In a first assay, the cummulus-oocytes complexes (COCs) were matured, fertilized and cultured for embryo development in PMS supplemented with bovine serum albumin (BSA), and in North Carolina State University-23 (NCSU-23) supplemented with fluid follicular, until blastocysts evaluation. In the assay 2, maturation and culture were performed in PMS using BSA or polyvinyl alcohol (PVA) in a 2 × 2 factorial arrangement (PMS-BSA/BSA, PMS-BSA/PVA, PMS-PVA/PVA, PMS-PVA/BSA). The PMS had a positive effect on the total cell number (58.04) and the decrease of the total lipids (49.4%) regarding the NCSU-23 medium (37.98 and 59.2% respectively; p<0.05). The percentage of monospermic fertilization was significantly lower (42.3%; p<0.05) when oocytes were matured with PMS-BSA than in PMS-PVA (52.6%). The supplementation of BSA in the PMS for embryo culture, increased the blastocyst development, the cell number of blastocysts and decreased the content of total lipids (36.8%, 46.9 and 49.6% respectively; p<0.05), in comparison with the supplementation of PVA in the PMS for embryo culture. These results suggest that the semi-defined culture media system developed by the National Genetic Resources Center (CNRG), have proved favorable effects on the total cell number and the decrease of total lipids of porcine blastocysts in vitro produced.  Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias INSTITUTO NACIONAL DE INVESTIGACIONES FORESTALES AGRÍCOLAS Y PECUARIAS INSTITUTO NACIONAL DE INVESTIGACIONES FORESTALES AGRÍCOLAS Y PECUARIAS 2020-02-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/pdf application/xml https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/4757 10.22319/rmcp.v11i1.4757 Revista Mexicana de Ciencias Pecuarias; Vol. 11, Núm. 1 (2020): Enero-Marzo; 102-115 Revista Mexicana de Ciencias Pecuarias; Vol. 11, Núm. 1 (2020): Enero-Marzo; 102-115 2448-6698 2007-1124 eng spa https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/4757/4241 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/4757/4242 https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/4757/4903 http://creativecommons.org/licenses/by-nc-sa/4.0

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