In vitro differentiation of preadipocytes from bovine adipose tissue

To study differentiation of bovine adipose tissue preadipocytes into adipocytes in bovines, two culture media were tested. Samples were obtained of subcutaneous and omentum fat from 3-4 yr old Holstein adults, and perirenal and omentum fat from fetuses in their last third of gestation. Although no differentiation was observed in cells obtained from adult animals' adipose tissue, fetal perirenal fibroblasts differentiated into adipocytes in the following medium: DMEM/F-12 1:1, 10 μ,,g/ml insulin, 0.25 μ,,M dexamethasone, 10 μ,,g/ml very low-density lipoproteins, 100 μ,,g/ml streptomycin, and 100 IU/ml penicillin. These results could be used to develop a model to study the molecular mechanisms of preadipocyte differentiation in bovine adipose tissue.