In vitro differentiation of preadipocytes from bovine adipose tissue

In vitro differentiation of preadipocytes from bovine adipose tissue

To study differentiation of bovine adipose tissue preadipocytes into adipocytes in bovines, two culture media were tested. Samples were obtained of subcutaneous and omentum fat from 3-4 yr old Holstein adults, and perirenal and omentum fat from fetuses in their last third of gestation. Although no differentiation was observed in cells obtained from adult animals' adipose tissue, fetal perirenal fibroblasts differentiated into adipocytes in the following medium: DMEM/F-12 1:1, 10 μ,,g/ml insulin, 0.25 μ,,M dexamethasone, 10 μ,,g/ml very low-density lipoproteins, 100 μ,,g/ml streptomycin, and 100 IU/ml penicillin. These results could be used to develop a model to study the molecular mechanisms of preadipocyte differentiation in bovine adipose tissue.

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Main Authors: González Gallardo, Adriana, Varela Echavarría, Alfredo, Shimada Miyasaka, Armando, Mora Izaguirre, Ofelia
Format: Digital revista
Language:spa
Published: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias 2012
Online Access:https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1811
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spelling rev-remexcp-article18112020-06-18T19:43:54Z In vitro differentiation of preadipocytes from bovine adipose tissue Diferenciación in vitro de preadipocitos de tejido adiposo bovino González Gallardo, Adriana Varela Echavarría, Alfredo Shimada Miyasaka, Armando Mora Izaguirre, Ofelia Bovine, Differentiation, Adipocytes Bovinos, Diferenciación, Adipocitos To study differentiation of bovine adipose tissue preadipocytes into adipocytes in bovines, two culture media were tested. Samples were obtained of subcutaneous and omentum fat from 3-4 yr old Holstein adults, and perirenal and omentum fat from fetuses in their last third of gestation. Although no differentiation was observed in cells obtained from adult animals' adipose tissue, fetal perirenal fibroblasts differentiated into adipocytes in the following medium: DMEM/F-12 1:1, 10 μ,,g/ml insulin, 0.25 μ,,M dexamethasone, 10 μ,,g/ml very low-density lipoproteins, 100 μ,,g/ml streptomycin, and 100 IU/ml penicillin. These results could be used to develop a model to study the molecular mechanisms of preadipocyte differentiation in bovine adipose tissue. Con objeto de obtener subcultivos seriales de preadipocitos del tejido adiposo bovino y luego tratar de diferenciarlos a adipocitos, se compararon dos medios de cultivo. Se obtuvieron muestras de tejido adiposo subcutáneo y de omento de animales adultos de 3 a 4 años de edad, y de grasa perirrenal y de omento de fetos en el íºltimo tercio de la gestación. En el caso de células adultas, se establecieron subcultivos, sin embargo no pudieron ser diferenciadas. Para el tejido adiposo fetal, se establecieron subcultivos y los fibroblastos perirrenales fueron capaces de diferenciarse con el medio apropiado (DMEM/ F-12 1:1, 10 μ,,g/ml insulina, 0.25 μ,,M dexametasona, 10 μ,,g/ml lipoproteí­nas de muy baja densidad, 100 μ,,g/ml estreptomicina, 100 UI/ml penicilina). Estos resultados podrí­an servir de base para obtener un modelo que permita estudiar los mecanismos moleculares de diferenciación de preadipocitos en tejido adiposo bovino. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias 2012-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1811 Revista Mexicana de Ciencias Pecuarias; Vol. 46, Núm. 2 (2008); 195 a 204 Revista Mexicana de Ciencias Pecuarias; Vol. 46, Núm. 2 (2008); 195 a 204 2448-6698 2007-1124 spa https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1811/1805 http://creativecommons.org/licenses/by-nc-sa/4.0
institution INIFAP
collection OJS
country México
countrycode MX
component Revista
access En linea
databasecode rev-remexcp
tag revista
region America del Norte
libraryname Biblioteca INIFAP
language spa
format Digital
author González Gallardo, Adriana
Varela Echavarría, Alfredo
Shimada Miyasaka, Armando
Mora Izaguirre, Ofelia
spellingShingle González Gallardo, Adriana
Varela Echavarría, Alfredo
Shimada Miyasaka, Armando
Mora Izaguirre, Ofelia
In vitro differentiation of preadipocytes from bovine adipose tissue
author_facet González Gallardo, Adriana
Varela Echavarría, Alfredo
Shimada Miyasaka, Armando
Mora Izaguirre, Ofelia
author_sort González Gallardo, Adriana
title In vitro differentiation of preadipocytes from bovine adipose tissue
title_short In vitro differentiation of preadipocytes from bovine adipose tissue
title_full In vitro differentiation of preadipocytes from bovine adipose tissue
title_fullStr In vitro differentiation of preadipocytes from bovine adipose tissue
title_full_unstemmed In vitro differentiation of preadipocytes from bovine adipose tissue
title_sort in vitro differentiation of preadipocytes from bovine adipose tissue
description To study differentiation of bovine adipose tissue preadipocytes into adipocytes in bovines, two culture media were tested. Samples were obtained of subcutaneous and omentum fat from 3-4 yr old Holstein adults, and perirenal and omentum fat from fetuses in their last third of gestation. Although no differentiation was observed in cells obtained from adult animals' adipose tissue, fetal perirenal fibroblasts differentiated into adipocytes in the following medium: DMEM/F-12 1:1, 10 μ,,g/ml insulin, 0.25 μ,,M dexamethasone, 10 μ,,g/ml very low-density lipoproteins, 100 μ,,g/ml streptomycin, and 100 IU/ml penicillin. These results could be used to develop a model to study the molecular mechanisms of preadipocyte differentiation in bovine adipose tissue.
publisher Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
publishDate 2012
url https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1811
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AT moraizaguirreofelia diferenciacioninvitrodepreadipocitosdetejidoadiposobovino
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