An analysis of two native poultry populations (Gallus domesticus) using RAPD's as molecular markers

An analysis of two native poultry populations (Gallus domesticus) using RAPD's as molecular markers

México has a great variety of native poultry but knowledge about its diversity is minimal. In this study, twenty individuals belonging to two populations of native hens (Gallus domesticus) were analyzed. They were chosen by egg production, through polymorphism identification generated by DNA random amplification (RAPD',,,s). Amplification generated products show different sizes between 0.2 to 1.1 kb. Polymorphism was detected between populations and inside populations, being the low egg production hen population the one which showed the lowest variability. Both populations show conserved products at 0.2 kb, 0.4 kb, 0.5 kb and 0.6 kb. The 1.1 kb fragment present in the lower egg production hen population did not show up in the other population, thus indicating that it could be a candidate as a genetic marker for characterization of hens with low egg production, or that the absence of this fragment could indicate hens with good egg production. The RAPD technique is a useful tool for identifying polymorphisms in a population and preliminary results allow to establish molecular variability between hen populations.

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Main Authors: Soto Huipe, Irma Morelia, Zavala Páramo, Guadalupe, Cano Camacho, Horacio, López Meza, Joel E.
Format: Digital revista
Language:spa
Published: Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias 2012
Online Access:https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1295
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spelling rev-remexcp-article12952020-06-18T18:56:15Z An analysis of two native poultry populations (Gallus domesticus) using RAPD's as molecular markers Análisis de dos poblaciones de gallinas criollas (Gallus domesticus) utilizando RAPD´s como marcadores moleculares Soto Huipe, Irma Morelia Zavala Páramo, Guadalupe Cano Camacho, Horacio López Meza, Joel E. Native chickens, Egg production, Molecular markers, RAPD's Gallinas criollas, Producción de huevo, Marcadores moleculares, RAPD´s México has a great variety of native poultry but knowledge about its diversity is minimal. In this study, twenty individuals belonging to two populations of native hens (Gallus domesticus) were analyzed. They were chosen by egg production, through polymorphism identification generated by DNA random amplification (RAPD',,,s). Amplification generated products show different sizes between 0.2 to 1.1 kb. Polymorphism was detected between populations and inside populations, being the low egg production hen population the one which showed the lowest variability. Both populations show conserved products at 0.2 kb, 0.4 kb, 0.5 kb and 0.6 kb. The 1.1 kb fragment present in the lower egg production hen population did not show up in the other population, thus indicating that it could be a candidate as a genetic marker for characterization of hens with low egg production, or that the absence of this fragment could indicate hens with good egg production. The RAPD technique is a useful tool for identifying polymorphisms in a population and preliminary results allow to establish molecular variability between hen populations. México cuenta con una enorme riqueza de gallinas criollas, sin embargo, el conocimiento acerca de su diversidad es mí­nimo. En el presente trabajo se analizaron 20 individuos de dos poblaciones de gallinas criollas (Gallus domesticus), seleccionadas basándose en su producción de huevos, mediante la identificación de polimorfismos generados por la amplificación aleatoria de ADN (RAPD's). Los productos de amplificación generados presentaron tamaños en el rango de 0.2 a 1.1 kilobases (kb). Se detectó polimorfismo entre las poblaciones y dentro de las mismas, siendo la población de gallinas de baja producción la que presentó menor variabilidad. Existe una conservación de los productos de 0.2, 0.4, 0.5 y 0.6 kb en las dos poblaciones. El fragmento de 1.1 kb presente en la población de gallinas de baja producción de huevo no se generó en la otra población, lo cual indica que pudiera ser considerado como un candidato a marcador genético para caracterizar aquellas gallinas malas ponedoras, o bien, que su ausencia determine que una gallina puede tener una buena producción de huevos. La técnica de RAPD',,,s es una herramienta íºtil para detectar polimorfismos en una población, y los resultados preliminares obtenidos permiten establecer en el ámbito molecular la variabilidad entre las poblaciones de gallinas. PALABRAS CLAVE: Gallinas criollas, Producción de huevo, Marcadores moleculares, RAPD´s. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias 2012-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1295 Revista Mexicana de Ciencias Pecuarias; Vol. 40, Núm. 3 (2002) Revista Mexicana de Ciencias Pecuarias; Vol. 40, Núm. 3 (2002) 2448-6698 2007-1124 spa https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1295/1290 http://creativecommons.org/licenses/by-nc-sa/4.0
institution INIFAP
collection OJS
country México
countrycode MX
component Revista
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databasecode rev-remexcp
tag revista
region America del Norte
libraryname Biblioteca INIFAP
language spa
format Digital
author Soto Huipe, Irma Morelia
Zavala Páramo, Guadalupe
Cano Camacho, Horacio
López Meza, Joel E.
spellingShingle Soto Huipe, Irma Morelia
Zavala Páramo, Guadalupe
Cano Camacho, Horacio
López Meza, Joel E.
An analysis of two native poultry populations (Gallus domesticus) using RAPD's as molecular markers
author_facet Soto Huipe, Irma Morelia
Zavala Páramo, Guadalupe
Cano Camacho, Horacio
López Meza, Joel E.
author_sort Soto Huipe, Irma Morelia
title An analysis of two native poultry populations (Gallus domesticus) using RAPD's as molecular markers
title_short An analysis of two native poultry populations (Gallus domesticus) using RAPD's as molecular markers
title_full An analysis of two native poultry populations (Gallus domesticus) using RAPD's as molecular markers
title_fullStr An analysis of two native poultry populations (Gallus domesticus) using RAPD's as molecular markers
title_full_unstemmed An analysis of two native poultry populations (Gallus domesticus) using RAPD's as molecular markers
title_sort analysis of two native poultry populations (gallus domesticus) using rapd's as molecular markers
description México has a great variety of native poultry but knowledge about its diversity is minimal. In this study, twenty individuals belonging to two populations of native hens (Gallus domesticus) were analyzed. They were chosen by egg production, through polymorphism identification generated by DNA random amplification (RAPD',,,s). Amplification generated products show different sizes between 0.2 to 1.1 kb. Polymorphism was detected between populations and inside populations, being the low egg production hen population the one which showed the lowest variability. Both populations show conserved products at 0.2 kb, 0.4 kb, 0.5 kb and 0.6 kb. The 1.1 kb fragment present in the lower egg production hen population did not show up in the other population, thus indicating that it could be a candidate as a genetic marker for characterization of hens with low egg production, or that the absence of this fragment could indicate hens with good egg production. The RAPD technique is a useful tool for identifying polymorphisms in a population and preliminary results allow to establish molecular variability between hen populations.
publisher Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias
publishDate 2012
url https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1295
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