Strategies for the efficiency of common bean selection for resistance to fusarium wilt

The objective of this work was to improve the efficiency of common bean lines selection for resistance to Fusarium oxysporum f.sp. phaseoli. Seven methods of inoculation were compared, using four common bean lines in a randomized complete design, in 4x7 factorial, with five replications. Lines reaction was evaluated at 21 days after inoculation. In order to identify the best date for severity evaluation, 18 common bean lines were inoculated through root immersion in a suspension of spores, with roots cut, in completely randomized design, with 15 replications. The disease progress was measured at 7, 14, 21 and 28 days after inoculation. The effect of number of replications from 5 to 15 was simulated, using data from 15 replications, with 20 common bean lines. The best methods to discriminate the lines were root immersions in a suspension of spores – with or without cut of the roots. Disease evaluation should be done at least 21 days after inoculation. Five replications are sufficient to classify the lines of common bean to fusarium wilt.

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Main Authors: Pereira, Mônica Juliani Zavaglia, Ramalho, Magno Antonio Patto, Abreu, Ângela de Fátima Barbosa
Format: Digital revista
Language:por
Published: Pesquisa Agropecuaria Brasileira 2008
Online Access:https://seer.sct.embrapa.br/index.php/pab/article/view/73
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spelling rev-pab-br-article-732014-07-02T17:45:28Z Strategies for the efficiency of common bean selection for resistance to fusarium wilt Estratégias para eficiência da seleção de feijoeiro quanto à resistência à murcha-de-fusário Pereira, Mônica Juliani Zavaglia Ramalho, Magno Antonio Patto Abreu, Ângela de Fátima Barbosa Fusarium oxysporum; evaluation times; inoculation; genetic improvement; severity Fusarium oxysporum; épocas de avaliação; inoculação; melhoramento genético; severidade The objective of this work was to improve the efficiency of common bean lines selection for resistance to Fusarium oxysporum f.sp. phaseoli. Seven methods of inoculation were compared, using four common bean lines in a randomized complete design, in 4x7 factorial, with five replications. Lines reaction was evaluated at 21 days after inoculation. In order to identify the best date for severity evaluation, 18 common bean lines were inoculated through root immersion in a suspension of spores, with roots cut, in completely randomized design, with 15 replications. The disease progress was measured at 7, 14, 21 and 28 days after inoculation. The effect of number of replications from 5 to 15 was simulated, using data from 15 replications, with 20 common bean lines. The best methods to discriminate the lines were root immersions in a suspension of spores – with or without cut of the roots. Disease evaluation should be done at least 21 days after inoculation. Five replications are sufficient to classify the lines of common bean to fusarium wilt. O objetivo deste trabalho foi melhorar a eficiência de seleção de linhagens de feijoeiro, para a resistência ao Fusarium oxysporum f.sp. phaseoli. Foram comparados sete métodos de inoculação do patógeno, em quatro linhagens de feijoeiro, em delineamento inteiramente ao acaso, em esquema fatorial 4x7, com cinco repetições. A reação das linhagens foi avaliada aos 21 dias após a inoculação. Para identificar a melhor época de avaliação da severidade do patógeno, 18 linhagens de feijoeiro foram submetidas à inoculação do patógeno, pela imersão de raízes na suspensão de esporos, com corte do sistema radicular, em delineamento inteiramente ao acaso, com 15 repetições. O progresso da doença foi acompanhado aos 7, 14, 21 e 28 dias após a inoculação. Com dados de experimento de 20 linhagens de feijoeiro com 15 repetições, foi simulado o efeito do número de repetições, que variou de 5 a 15. Os métodos que melhor discriminaram as linhagens foram os de imersão de raízes na suspensão de conídios - com ou sem o corte do sistema radicular. As avaliações da reação à doença devem ser realizadas com pelo menos 21 dias após a inoculação. Cinco repetições são suficientes para classificar as linhagens de feijoeiro, quanto à resistência à murcha-de-fusário. Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira CNPq 2008-10-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://seer.sct.embrapa.br/index.php/pab/article/view/73 Pesquisa Agropecuaria Brasileira; v.43, n.6, jun. 2008; 721-728 Pesquisa Agropecuária Brasileira; v.43, n.6, jun. 2008; 721-728 1678-3921 0100-104x por https://seer.sct.embrapa.br/index.php/pab/article/view/73/5449 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/73/43
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country Brasil
countrycode BR
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databasecode rev-pab-br
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libraryname Sistema de bibliotecas de EMBRAPA
language por
format Digital
author Pereira, Mônica Juliani Zavaglia
Ramalho, Magno Antonio Patto
Abreu, Ângela de Fátima Barbosa
spellingShingle Pereira, Mônica Juliani Zavaglia
Ramalho, Magno Antonio Patto
Abreu, Ângela de Fátima Barbosa
Strategies for the efficiency of common bean selection for resistance to fusarium wilt
author_facet Pereira, Mônica Juliani Zavaglia
Ramalho, Magno Antonio Patto
Abreu, Ângela de Fátima Barbosa
author_sort Pereira, Mônica Juliani Zavaglia
title Strategies for the efficiency of common bean selection for resistance to fusarium wilt
title_short Strategies for the efficiency of common bean selection for resistance to fusarium wilt
title_full Strategies for the efficiency of common bean selection for resistance to fusarium wilt
title_fullStr Strategies for the efficiency of common bean selection for resistance to fusarium wilt
title_full_unstemmed Strategies for the efficiency of common bean selection for resistance to fusarium wilt
title_sort strategies for the efficiency of common bean selection for resistance to fusarium wilt
description The objective of this work was to improve the efficiency of common bean lines selection for resistance to Fusarium oxysporum f.sp. phaseoli. Seven methods of inoculation were compared, using four common bean lines in a randomized complete design, in 4x7 factorial, with five replications. Lines reaction was evaluated at 21 days after inoculation. In order to identify the best date for severity evaluation, 18 common bean lines were inoculated through root immersion in a suspension of spores, with roots cut, in completely randomized design, with 15 replications. The disease progress was measured at 7, 14, 21 and 28 days after inoculation. The effect of number of replications from 5 to 15 was simulated, using data from 15 replications, with 20 common bean lines. The best methods to discriminate the lines were root immersions in a suspension of spores – with or without cut of the roots. Disease evaluation should be done at least 21 days after inoculation. Five replications are sufficient to classify the lines of common bean to fusarium wilt.
publisher Pesquisa Agropecuaria Brasileira
publishDate 2008
url https://seer.sct.embrapa.br/index.php/pab/article/view/73
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