Strategies for the efficiency of common bean selection for resistance to fusarium wilt
The objective of this work was to improve the efficiency of common bean lines selection for resistance to Fusarium oxysporum f.sp. phaseoli. Seven methods of inoculation were compared, using four common bean lines in a randomized complete design, in 4x7 factorial, with five replications. Lines reaction was evaluated at 21 days after inoculation. In order to identify the best date for severity evaluation, 18 common bean lines were inoculated through root immersion in a suspension of spores, with roots cut, in completely randomized design, with 15 replications. The disease progress was measured at 7, 14, 21 and 28 days after inoculation. The effect of number of replications from 5 to 15 was simulated, using data from 15 replications, with 20 common bean lines. The best methods to discriminate the lines were root immersions in a suspension of spores – with or without cut of the roots. Disease evaluation should be done at least 21 days after inoculation. Five replications are sufficient to classify the lines of common bean to fusarium wilt.
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Pesquisa Agropecuaria Brasileira
2008
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rev-pab-br-article-732014-07-02T17:45:28Z Strategies for the efficiency of common bean selection for resistance to fusarium wilt Estratégias para eficiência da seleção de feijoeiro quanto à resistência à murcha-de-fusário Pereira, Mônica Juliani Zavaglia Ramalho, Magno Antonio Patto Abreu, Ângela de Fátima Barbosa Fusarium oxysporum; evaluation times; inoculation; genetic improvement; severity Fusarium oxysporum; épocas de avaliação; inoculação; melhoramento genético; severidade The objective of this work was to improve the efficiency of common bean lines selection for resistance to Fusarium oxysporum f.sp. phaseoli. Seven methods of inoculation were compared, using four common bean lines in a randomized complete design, in 4x7 factorial, with five replications. Lines reaction was evaluated at 21 days after inoculation. In order to identify the best date for severity evaluation, 18 common bean lines were inoculated through root immersion in a suspension of spores, with roots cut, in completely randomized design, with 15 replications. The disease progress was measured at 7, 14, 21 and 28 days after inoculation. The effect of number of replications from 5 to 15 was simulated, using data from 15 replications, with 20 common bean lines. The best methods to discriminate the lines were root immersions in a suspension of spores – with or without cut of the roots. Disease evaluation should be done at least 21 days after inoculation. Five replications are sufficient to classify the lines of common bean to fusarium wilt. O objetivo deste trabalho foi melhorar a eficiência de seleção de linhagens de feijoeiro, para a resistência ao Fusarium oxysporum f.sp. phaseoli. Foram comparados sete métodos de inoculação do patógeno, em quatro linhagens de feijoeiro, em delineamento inteiramente ao acaso, em esquema fatorial 4x7, com cinco repetições. A reação das linhagens foi avaliada aos 21 dias após a inoculação. Para identificar a melhor época de avaliação da severidade do patógeno, 18 linhagens de feijoeiro foram submetidas à inoculação do patógeno, pela imersão de raízes na suspensão de esporos, com corte do sistema radicular, em delineamento inteiramente ao acaso, com 15 repetições. O progresso da doença foi acompanhado aos 7, 14, 21 e 28 dias após a inoculação. Com dados de experimento de 20 linhagens de feijoeiro com 15 repetições, foi simulado o efeito do número de repetições, que variou de 5 a 15. Os métodos que melhor discriminaram as linhagens foram os de imersão de raízes na suspensão de conídios - com ou sem o corte do sistema radicular. As avaliações da reação à doença devem ser realizadas com pelo menos 21 dias após a inoculação. Cinco repetições são suficientes para classificar as linhagens de feijoeiro, quanto à resistência à murcha-de-fusário. Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira CNPq 2008-10-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://seer.sct.embrapa.br/index.php/pab/article/view/73 Pesquisa Agropecuaria Brasileira; v.43, n.6, jun. 2008; 721-728 Pesquisa Agropecuária Brasileira; v.43, n.6, jun. 2008; 721-728 1678-3921 0100-104x por https://seer.sct.embrapa.br/index.php/pab/article/view/73/5449 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/73/43 |
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Pereira, Mônica Juliani Zavaglia Ramalho, Magno Antonio Patto Abreu, Ângela de Fátima Barbosa |
spellingShingle |
Pereira, Mônica Juliani Zavaglia Ramalho, Magno Antonio Patto Abreu, Ângela de Fátima Barbosa Strategies for the efficiency of common bean selection for resistance to fusarium wilt |
author_facet |
Pereira, Mônica Juliani Zavaglia Ramalho, Magno Antonio Patto Abreu, Ângela de Fátima Barbosa |
author_sort |
Pereira, Mônica Juliani Zavaglia |
title |
Strategies for the efficiency of common bean selection for resistance to fusarium wilt |
title_short |
Strategies for the efficiency of common bean selection for resistance to fusarium wilt |
title_full |
Strategies for the efficiency of common bean selection for resistance to fusarium wilt |
title_fullStr |
Strategies for the efficiency of common bean selection for resistance to fusarium wilt |
title_full_unstemmed |
Strategies for the efficiency of common bean selection for resistance to fusarium wilt |
title_sort |
strategies for the efficiency of common bean selection for resistance to fusarium wilt |
description |
The objective of this work was to improve the efficiency of common bean lines selection for resistance to Fusarium oxysporum f.sp. phaseoli. Seven methods of inoculation were compared, using four common bean lines in a randomized complete design, in 4x7 factorial, with five replications. Lines reaction was evaluated at 21 days after inoculation. In order to identify the best date for severity evaluation, 18 common bean lines were inoculated through root immersion in a suspension of spores, with roots cut, in completely randomized design, with 15 replications. The disease progress was measured at 7, 14, 21 and 28 days after inoculation. The effect of number of replications from 5 to 15 was simulated, using data from 15 replications, with 20 common bean lines. The best methods to discriminate the lines were root immersions in a suspension of spores – with or without cut of the roots. Disease evaluation should be done at least 21 days after inoculation. Five replications are sufficient to classify the lines of common bean to fusarium wilt. |
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Pesquisa Agropecuaria Brasileira |
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2008 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/73 |
work_keys_str_mv |
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