Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers
The objective of this work was to evaluate the genetic diversity in a population of 148 hybrids of 'Murcott' tangor (Citrus reticulata Blanco x C. sinensis L. Osbeck) and 'Pêra' sweet orange (C. sinensis L. Osbeck), obtained by controlled polination, using fAFLP and RAPD markers. Polymorphic markers (416 fAFLP and 33 RAPD markers) were used to evaluate genetic similarity among the hybrids, calculated by the coefficient of Jaccard, using the UPGMA method. The consistency of each group was determined by software BOOD. There was high genetic similarity within the parents. 'Pêra' sweet orange had a higher number of loci in heterozygosis (132) compared to 'Murcott' tangor (105), supporting the theory of hybrid origin for sweet oranges. Two distinct groups of plants were observed: one group had 80% of the hybrids that displayed higher genetic similarity with 'Pêra' sweet orange. The bootstrap analysis did not show consistence between the groups. The fAFLP markers are more efficient to obtain polymorphism than RAPDs and are more suitable to select hybrids closer to the parents.
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Pesquisa Agropecuaria Brasileira
2006
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rev-pab-br-article-72062010-12-02T14:10:49Z Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers Diversidade genética entre híbridos de laranja-doce e tangor 'Murcott' avaliada por fAFLP e RAPD Bastianel, Marinês Oliveira, Antonio Carlos de Cristofani, Mariângela Machado, Marcos Antônio citrus; molecular markers; genetic similarity citros; marcadores moleculares; similaridade genética The objective of this work was to evaluate the genetic diversity in a population of 148 hybrids of 'Murcott' tangor (Citrus reticulata Blanco x C. sinensis L. Osbeck) and 'Pêra' sweet orange (C. sinensis L. Osbeck), obtained by controlled polination, using fAFLP and RAPD markers. Polymorphic markers (416 fAFLP and 33 RAPD markers) were used to evaluate genetic similarity among the hybrids, calculated by the coefficient of Jaccard, using the UPGMA method. The consistency of each group was determined by software BOOD. There was high genetic similarity within the parents. 'Pêra' sweet orange had a higher number of loci in heterozygosis (132) compared to 'Murcott' tangor (105), supporting the theory of hybrid origin for sweet oranges. Two distinct groups of plants were observed: one group had 80% of the hybrids that displayed higher genetic similarity with 'Pêra' sweet orange. The bootstrap analysis did not show consistence between the groups. The fAFLP markers are more efficient to obtain polymorphism than RAPDs and are more suitable to select hybrids closer to the parents. O objetivo deste trabalho foi avaliar a diversidade genética em uma população de 148 híbridos de tangor 'Murcott' (Citrus reticulata Blanco x C. sinensis L. Osbeck) e laranja 'Pêra' (C. sinensis L. Osbeck) obtidos por polinização controlada, pelo uso de marcadores fAFLP e RAPD. Marcadores polimórficos (416 marcadores fAFLP e 33 RAPD) foram utilizados para avaliar a similaridade genética entre os híbridos, calculada com o coeficiente Jaccard pelo método UPGMA. A consistência de cada agrupamento foi determinada pelo programa BOOD. Houve alta similaridade genética entre os parentais. A laranja 'Pêra' apresentou maior número (132) de loci em heterozigose em relação ao tangor 'Murcott' (105), corroborando a teoria de origem híbrida para a laranja-doce. Observaram-se dois grupos distintos de plantas, e um deles abrangeu 80% dos híbridos com maior similaridade com a laranja 'Pêra'. A análise bootstrap não revelou consistência estatística entre esses grupos. Marcadores fAFLP são mais eficientes na avaliação do polimorfismo, sendo indicados para seleção de indivíduos híbridos mais próximos a um dos parentais. Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira 2006-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://seer.sct.embrapa.br/index.php/pab/article/view/7206 Pesquisa Agropecuaria Brasileira; v.41, n.5, maio 2006; 779-784 Pesquisa Agropecuária Brasileira; v.41, n.5, maio 2006; 779-784 1678-3921 0100-104x por https://seer.sct.embrapa.br/index.php/pab/article/view/7206/4251 |
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Bastianel, Marinês Oliveira, Antonio Carlos de Cristofani, Mariângela Machado, Marcos Antônio |
| spellingShingle |
Bastianel, Marinês Oliveira, Antonio Carlos de Cristofani, Mariângela Machado, Marcos Antônio Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers |
| author_facet |
Bastianel, Marinês Oliveira, Antonio Carlos de Cristofani, Mariângela Machado, Marcos Antônio |
| author_sort |
Bastianel, Marinês |
| title |
Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers |
| title_short |
Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers |
| title_full |
Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers |
| title_fullStr |
Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers |
| title_full_unstemmed |
Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers |
| title_sort |
genetic diversity among hybrids of sweet orange and 'murcott' tangor evaluated by faflp and rapd markers |
| description |
The objective of this work was to evaluate the genetic diversity in a population of 148 hybrids of 'Murcott' tangor (Citrus reticulata Blanco x C. sinensis L. Osbeck) and 'Pêra' sweet orange (C. sinensis L. Osbeck), obtained by controlled polination, using fAFLP and RAPD markers. Polymorphic markers (416 fAFLP and 33 RAPD markers) were used to evaluate genetic similarity among the hybrids, calculated by the coefficient of Jaccard, using the UPGMA method. The consistency of each group was determined by software BOOD. There was high genetic similarity within the parents. 'Pêra' sweet orange had a higher number of loci in heterozygosis (132) compared to 'Murcott' tangor (105), supporting the theory of hybrid origin for sweet oranges. Two distinct groups of plants were observed: one group had 80% of the hybrids that displayed higher genetic similarity with 'Pêra' sweet orange. The bootstrap analysis did not show consistence between the groups. The fAFLP markers are more efficient to obtain polymorphism than RAPDs and are more suitable to select hybrids closer to the parents. |
| publisher |
Pesquisa Agropecuaria Brasileira |
| publishDate |
2006 |
| url |
https://seer.sct.embrapa.br/index.php/pab/article/view/7206 |
| work_keys_str_mv |
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