Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium

Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium

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The objective of this work was to evaluate the susceptibility to glufosinate ammonium of transgenic plants of Swingle citrumelo with high proline production. The mutant gene of the enzyme D1-pyrroline-5-carboxylate synthetase (P5CS), the rate-limiting enzyme in proline biosynthesis, was inserted into Swingle citrumelo plants. The expression of the gene p5cs caused up to 5-fold increase on the proline content in leaf tissues of transgenic plants treated with 200 mM glufosinate ammonium, when compared with control plants. Leaves of transgenic plants accumulated higher amounts of NH4+ than the nontransgenic control. The herbicide toxicity was evaluated using leaf disks cultivated in MS medium, containing different concentrations of glufosinate ammonium. The severity of the chlorosis, observed in leaf disks of transgenic plants, confirmed the higher susceptibility of Swingle citrumelo plants, with high proline production, to this herbicide.

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Main Authors: Carneiro, Cristine Elizabeth Alvarenga, Molinari, Hugo Bruno Correa, Andrade, Giselly Aparecida, Pereira, Luiz Filipe Protasio, Vieira, Luiz Gonzaga Esteves
Format: Digital revista
Language:por
Published: Pesquisa Agropecuaria Brasileira 2006
Online Access:https://seer.sct.embrapa.br/index.php/pab/article/view/7202
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spelling rev-pab-br-article-72022014-09-29T18:21:07Z Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium Produção de prolina e suscetibilidade ao glufosinato de amônio em plantas transgênicas de citrumelo Swingle Carneiro, Cristine Elizabeth Alvarenga Molinari, Hugo Bruno Correa Andrade, Giselly Aparecida Pereira, Luiz Filipe Protasio Vieira, Luiz Gonzaga Esteves Citrus paradisi; Poncirus trifoliata; phosphinothricin; P5CS Citrus paradisi; Poncirus trifoliata; fosfinotricina; P5CS The objective of this work was to evaluate the susceptibility to glufosinate ammonium of transgenic plants of Swingle citrumelo with high proline production. The mutant gene of the enzyme D1-pyrroline-5-carboxylate synthetase (P5CS), the rate-limiting enzyme in proline biosynthesis, was inserted into Swingle citrumelo plants. The expression of the gene p5cs caused up to 5-fold increase on the proline content in leaf tissues of transgenic plants treated with 200 mM glufosinate ammonium, when compared with control plants. Leaves of transgenic plants accumulated higher amounts of NH4+ than the nontransgenic control. The herbicide toxicity was evaluated using leaf disks cultivated in MS medium, containing different concentrations of glufosinate ammonium. The severity of the chlorosis, observed in leaf disks of transgenic plants, confirmed the higher susceptibility of Swingle citrumelo plants, with high proline production, to this herbicide. O objetivo deste trabalho foi avaliar a sensibilidade de plantas transgênicas de citrumelo Swingle com elevada produção de prolina, ao herbicida glufosinato de amônio. As plantas utilizadas apresentavam a inserção do gene mutante da enzima D1-pirrolina-5-carboxilato sintetase (P5CS), responsável pela biossíntese de prolina. A expressão do gene p5cs em plantas transgênicas causou aumento nas quantidades de prolina em tecidos foliares, em até cinco vezes, quando comparadas às plantas-controle tratadas com 200 mM de glufosinato de amônio. As plantas transgênicas acumularam maior quantidade de NH4+ nas folhas, em relação às plantas não-transgênicas. Os danos causados pelo herbicida foram avaliados in vitro, utilizando-se discos foliares cultivados em meio MS com diferentes concentrações de glufosinato de amônio. Observou-se maior clorose em discos foliares das plantas transgênicas, o que comprova a maior suscetibilidade de plantas de citrumelo Swingle com alta produção de prolina ao herbicida. Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira 2006-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://seer.sct.embrapa.br/index.php/pab/article/view/7202 Pesquisa Agropecuaria Brasileira; v.41, n.5, maio 2006; 747-753 Pesquisa Agropecuária Brasileira; v.41, n.5, maio 2006; 747-753 1678-3921 0100-104x por https://seer.sct.embrapa.br/index.php/pab/article/view/7202/4247
institution EMBRAPA
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-pab-br
tag revista
region America del Sur
libraryname Sistema de bibliotecas de EMBRAPA
language por
format Digital
author Carneiro, Cristine Elizabeth Alvarenga
Molinari, Hugo Bruno Correa
Andrade, Giselly Aparecida
Pereira, Luiz Filipe Protasio
Vieira, Luiz Gonzaga Esteves
spellingShingle Carneiro, Cristine Elizabeth Alvarenga
Molinari, Hugo Bruno Correa
Andrade, Giselly Aparecida
Pereira, Luiz Filipe Protasio
Vieira, Luiz Gonzaga Esteves
Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
author_facet Carneiro, Cristine Elizabeth Alvarenga
Molinari, Hugo Bruno Correa
Andrade, Giselly Aparecida
Pereira, Luiz Filipe Protasio
Vieira, Luiz Gonzaga Esteves
author_sort Carneiro, Cristine Elizabeth Alvarenga
title Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
title_short Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
title_full Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
title_fullStr Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
title_full_unstemmed Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium
title_sort proline production by transgenic plants of swingle citrumelo and susceptibility to glufosinate ammonium
description The objective of this work was to evaluate the susceptibility to glufosinate ammonium of transgenic plants of Swingle citrumelo with high proline production. The mutant gene of the enzyme D1-pyrroline-5-carboxylate synthetase (P5CS), the rate-limiting enzyme in proline biosynthesis, was inserted into Swingle citrumelo plants. The expression of the gene p5cs caused up to 5-fold increase on the proline content in leaf tissues of transgenic plants treated with 200 mM glufosinate ammonium, when compared with control plants. Leaves of transgenic plants accumulated higher amounts of NH4+ than the nontransgenic control. The herbicide toxicity was evaluated using leaf disks cultivated in MS medium, containing different concentrations of glufosinate ammonium. The severity of the chlorosis, observed in leaf disks of transgenic plants, confirmed the higher susceptibility of Swingle citrumelo plants, with high proline production, to this herbicide.
publisher Pesquisa Agropecuaria Brasileira
publishDate 2006
url https://seer.sct.embrapa.br/index.php/pab/article/view/7202
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