In vitro induction and culture of adventitious buds in epicotyl segments of sour orange

In vitro induction and culture of adventitious buds in epicotyl segments of sour orange

Show other versions (1)

The objective of this work was to evaluate the induction and formation of adventitious buds in sour orange explants through the use of plant regulators. In vitro organogenesis experiments were conducted to evaluate the effect of BAP, TDZ, and KIN in different concentrations and under two light conditions; BAP and KIN, combined or not with NAA; BAP and KIN, separately or in combined concentrations. Sour orange epicotyl segments (1 cm length), from in vitro germinated plants, were used as explants. In order to induce bud formation, the explants were cultured in MT medium with or without the addition of plant regulators. The material was cultivated at 27ºC in the absence of light for 30 days, followed of culture under a 16-hour photoperiod. The experimental design was completely randomized, with four or five replicates, depending on the experiment; each replicate comprised one Petri dish with 20 explants. After 60 or 70 days of cultivation, the percentage of responsive explants and the number of buds were evaluated. The addition of BAP to the culture medium, combined or not with ANA, and in combinations with CIN, induces better organogenic response.

Saved in:
Bibliographic Details
Main Authors: Silva, Rosely Pereira da, Mendes, Beatriz Madalena Januzzi, Filho, Francisco de Assis Alves Mourão
Format: Digital revista
Language:por
Published: Pesquisa Agropecuaria Brasileira 2008
Online Access:https://seer.sct.embrapa.br/index.php/pab/article/view/710
Tags: Add Tag
No Tags, Be the first to tag this record!
id rev-pab-br-article-710
record_format ojs
spelling rev-pab-br-article-7102014-07-17T17:24:01Z In vitro induction and culture of adventitious buds in epicotyl segments of sour orange Indução e cultivo in vitro de gemas adventícias em segmentos de epicótilo de laranja-azeda Silva, Rosely Pereira da Mendes, Beatriz Madalena Januzzi Filho, Francisco de Assis Alves Mourão Citrus aurantium; organogenesis; growth regulator; micropropagation Citrus aurantium; fitorregulador; micropropagação; organogênese The objective of this work was to evaluate the induction and formation of adventitious buds in sour orange explants through the use of plant regulators. In vitro organogenesis experiments were conducted to evaluate the effect of BAP, TDZ, and KIN in different concentrations and under two light conditions; BAP and KIN, combined or not with NAA; BAP and KIN, separately or in combined concentrations. Sour orange epicotyl segments (1 cm length), from in vitro germinated plants, were used as explants. In order to induce bud formation, the explants were cultured in MT medium with or without the addition of plant regulators. The material was cultivated at 27ºC in the absence of light for 30 days, followed of culture under a 16-hour photoperiod. The experimental design was completely randomized, with four or five replicates, depending on the experiment; each replicate comprised one Petri dish with 20 explants. After 60 or 70 days of cultivation, the percentage of responsive explants and the number of buds were evaluated. The addition of BAP to the culture medium, combined or not with ANA, and in combinations with CIN, induces better organogenic response. O objetivo deste trabalho foi avaliar a indução e a formação de gemas adventícias em explantes de laranja-azeda, pelo uso de fi torreguladores. Em experimentos de organogênese in vitro foram avaliados 6-benzilaminopurina (BAP), thidiazuron (TDZ) e cinetina (CIN), cem diferentes concentrações e sob duas ondições de luminosidade; BAP e CIN combinados ou não com ácido naftalenoacético (ANA); e BAP eCIN isoladamente ou combinados entre si. Segmentos de epicótilo de 1 cm de comprimento, provenientes de plântulas de laranja-azeda germinadas in vitro, foram utilizados como explantes. Para induzir a formação de gemas, os segmentos foram cultivados em meio MT com ou sem adição de fi torreguladores. O material foi cultivado a 27ºC em ausência de luz por 30 dias, seguidos de fotoperíodo de 16 horas. O delineamento experimental foi inteiramente casualizado, com quatro ou cinco repetições, a depender do experimento e, cadarepetição foi constituída de placa de Petri com 20 explantes. Após 60 ou 70 dias de cultivo foram avaliados o percentual de explantes responsivos e o número de gemas por explante. A adição de BAP ao meio de cultura,combinada ou não com ANA, e em combinações com CIN promovem melhor resposta organogênica. Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira CNPq 2008-10-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://seer.sct.embrapa.br/index.php/pab/article/view/710 Pesquisa Agropecuaria Brasileira; v.43, n.10, out. 2008; 1331-1337 Pesquisa Agropecuária Brasileira; v.43, n.10, out. 2008; 1331-1337 1678-3921 0100-104x por https://seer.sct.embrapa.br/index.php/pab/article/view/710/5519
institution EMBRAPA
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-pab-br
tag revista
region America del Sur
libraryname Sistema de bibliotecas de EMBRAPA
language por
format Digital
author Silva, Rosely Pereira da
Mendes, Beatriz Madalena Januzzi
Filho, Francisco de Assis Alves Mourão
spellingShingle Silva, Rosely Pereira da
Mendes, Beatriz Madalena Januzzi
Filho, Francisco de Assis Alves Mourão
In vitro induction and culture of adventitious buds in epicotyl segments of sour orange
author_facet Silva, Rosely Pereira da
Mendes, Beatriz Madalena Januzzi
Filho, Francisco de Assis Alves Mourão
author_sort Silva, Rosely Pereira da
title In vitro induction and culture of adventitious buds in epicotyl segments of sour orange
title_short In vitro induction and culture of adventitious buds in epicotyl segments of sour orange
title_full In vitro induction and culture of adventitious buds in epicotyl segments of sour orange
title_fullStr In vitro induction and culture of adventitious buds in epicotyl segments of sour orange
title_full_unstemmed In vitro induction and culture of adventitious buds in epicotyl segments of sour orange
title_sort in vitro induction and culture of adventitious buds in epicotyl segments of sour orange
description The objective of this work was to evaluate the induction and formation of adventitious buds in sour orange explants through the use of plant regulators. In vitro organogenesis experiments were conducted to evaluate the effect of BAP, TDZ, and KIN in different concentrations and under two light conditions; BAP and KIN, combined or not with NAA; BAP and KIN, separately or in combined concentrations. Sour orange epicotyl segments (1 cm length), from in vitro germinated plants, were used as explants. In order to induce bud formation, the explants were cultured in MT medium with or without the addition of plant regulators. The material was cultivated at 27ºC in the absence of light for 30 days, followed of culture under a 16-hour photoperiod. The experimental design was completely randomized, with four or five replicates, depending on the experiment; each replicate comprised one Petri dish with 20 explants. After 60 or 70 days of cultivation, the percentage of responsive explants and the number of buds were evaluated. The addition of BAP to the culture medium, combined or not with ANA, and in combinations with CIN, induces better organogenic response.
publisher Pesquisa Agropecuaria Brasileira
publishDate 2008
url https://seer.sct.embrapa.br/index.php/pab/article/view/710
work_keys_str_mv AT silvaroselypereirada invitroinductionandcultureofadventitiousbudsinepicotylsegmentsofsourorange
AT mendesbeatrizmadalenajanuzzi invitroinductionandcultureofadventitiousbudsinepicotylsegmentsofsourorange
AT filhofranciscodeassisalvesmourao invitroinductionandcultureofadventitiousbudsinepicotylsegmentsofsourorange
AT silvaroselypereirada inducaoecultivoinvitrodegemasadventiciasemsegmentosdeepicotilodelaranjaazeda
AT mendesbeatrizmadalenajanuzzi inducaoecultivoinvitrodegemasadventiciasemsegmentosdeepicotilodelaranjaazeda
AT filhofranciscodeassisalvesmourao inducaoecultivoinvitrodegemasadventiciasemsegmentosdeepicotilodelaranjaazeda
_version_ 1813449965334167552

Similar Items