PCR detection of Clostridium chauvoei in tissues of Cavia porcellus

PCR detection of Clostridium chauvoei in tissues of Cavia porcellus

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The objective of this work was the standardization of a polymerase chain reaction (PCR) for detection of Clostridium chauvoei in formalin-fixed, paraffin-embedded tissues of guinea-pigs (Cavia porcellus) infected experimentally with this microorganism. The animals were sacrificed, and samples of muscle from inoculation area (MIA), liver, myocardium and spleen were available for PCR technique. Clostridium chauvoei was detected in all sections of the MIA, liver and myocardium, and no product was observed in sections of the spleen. Cross-reactions were not observed in sections of MIA of the animals inoculated with other clostridia, as well as no amplification was observed in sections of MIA of control animals. These results show that the PCR technique developed in this study may be useful for detection of C. chauvoei in formalin-fixed, paraffin-embedded tissues.

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Main Authors: Assis, Ronnie Antunes de, Lobato, Francisco Carlos Faria, Miyakawa, Mariano Fernandez, Uzal, Francisco Alejandro
Format: Digital revista
Language:por
Published: Pesquisa Agropecuaria Brasileira 2005
Online Access:https://seer.sct.embrapa.br/index.php/pab/article/view/7092
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spelling rev-pab-br-article-70922010-10-07T14:54:29Z PCR detection of Clostridium chauvoei in tissues of Cavia porcellus Reação em cadeia da polimerase para detecção de Clostridium chauvoei em tecidos de Cavia porcellus Assis, Ronnie Antunes de Lobato, Francisco Carlos Faria Miyakawa, Mariano Fernandez Uzal, Francisco Alejandro blackleg; malignant edema carbúnculo sintomático; edema maligno The objective of this work was the standardization of a polymerase chain reaction (PCR) for detection of Clostridium chauvoei in formalin-fixed, paraffin-embedded tissues of guinea-pigs (Cavia porcellus) infected experimentally with this microorganism. The animals were sacrificed, and samples of muscle from inoculation area (MIA), liver, myocardium and spleen were available for PCR technique. Clostridium chauvoei was detected in all sections of the MIA, liver and myocardium, and no product was observed in sections of the spleen. Cross-reactions were not observed in sections of MIA of the animals inoculated with other clostridia, as well as no amplification was observed in sections of MIA of control animals. These results show that the PCR technique developed in this study may be useful for detection of C. chauvoei in formalin-fixed, paraffin-embedded tissues. O objetivo deste trabalho foi padronizar uma técnica de reação em cadeia da polimerase (PCR), para detecção de Clostridium chauvoei, em tecidos fixados em formol e incluídos em parafina de cobaias (Cavia porcellus) experimentalmente infectadas com esse microrganismo. Os animais foram sacrificados, e amostras do músculo da área de inoculação (MAI), fígado, miocárdio e baço foram disponibilizadas para a técnica de PCR. O clostrídio foi detectado em todas as secções do MAI, fígado e miocárdio, mas não foi observado em secções do baço. Reações cruzadas não foram observadas a partir de secções do MAI dos animais com inoculação de outras espécies de clostrídios, bem como nenhuma amplificação foi observada a partir de secções do MAI dos animais controle. Esses resultados mostram que a técnica de PCR desenvolvida neste estudo, pode ser usada para detecção de Clostridium chauvoei em tecidos fixados em formol e incluídos em parafina. Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira 2005-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://seer.sct.embrapa.br/index.php/pab/article/view/7092 Pesquisa Agropecuaria Brasileira; v.40, n.12, dez. 2005; 1247-1250 Pesquisa Agropecuária Brasileira; v.40, n.12, dez. 2005; 1247-1250 1678-3921 0100-104x por https://seer.sct.embrapa.br/index.php/pab/article/view/7092/4140
institution EMBRAPA
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-pab-br
tag revista
region America del Sur
libraryname Sistema de bibliotecas de EMBRAPA
language por
format Digital
author Assis, Ronnie Antunes de
Lobato, Francisco Carlos Faria
Miyakawa, Mariano Fernandez
Uzal, Francisco Alejandro
spellingShingle Assis, Ronnie Antunes de
Lobato, Francisco Carlos Faria
Miyakawa, Mariano Fernandez
Uzal, Francisco Alejandro
PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
author_facet Assis, Ronnie Antunes de
Lobato, Francisco Carlos Faria
Miyakawa, Mariano Fernandez
Uzal, Francisco Alejandro
author_sort Assis, Ronnie Antunes de
title PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
title_short PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
title_full PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
title_fullStr PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
title_full_unstemmed PCR detection of Clostridium chauvoei in tissues of Cavia porcellus
title_sort pcr detection of clostridium chauvoei in tissues of cavia porcellus
description The objective of this work was the standardization of a polymerase chain reaction (PCR) for detection of Clostridium chauvoei in formalin-fixed, paraffin-embedded tissues of guinea-pigs (Cavia porcellus) infected experimentally with this microorganism. The animals were sacrificed, and samples of muscle from inoculation area (MIA), liver, myocardium and spleen were available for PCR technique. Clostridium chauvoei was detected in all sections of the MIA, liver and myocardium, and no product was observed in sections of the spleen. Cross-reactions were not observed in sections of MIA of the animals inoculated with other clostridia, as well as no amplification was observed in sections of MIA of control animals. These results show that the PCR technique developed in this study may be useful for detection of C. chauvoei in formalin-fixed, paraffin-embedded tissues.
publisher Pesquisa Agropecuaria Brasileira
publishDate 2005
url https://seer.sct.embrapa.br/index.php/pab/article/view/7092
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