Selection processes in a citrus hybrid population using RAPD markers
The objective of this work was to evaluate the processes of selection in a citrus hybrid population using segregation analysis of RAPD markers. The segregation of 123 RAPD markers between 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck) was analysed in a F1 progeny of 94 hybrids. Genetic composition, diversity, heterozygosity, differences in chromosomal structure and the presence of deleterious recessive genes are discussed based on the segregation ratios obtained. A high percentage of markers had a skeweness of the 1:1 expected segregation ratio in the F1 population. Many markers showed a 3:1 segregation ratio in both varieties and 1:3 in 'Pêra' sweet orange, probably due to directional selection processes. The distribution analysis of the frequencies of the segregant markers in a hybrid population is a simple method which allows a better understanding of the genetics of citrus group.
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Pesquisa Agropecuaria Brasileira
2003
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rev-pab-br-article-67032014-08-11T19:44:38Z Selection processes in a citrus hybrid population using RAPD markers Processos de seleção em uma população de híbridos de citros usando marcadores RAPD Oliveira, Roberto Pedroso de Vildoso, Carlos Ivan Aguilar Machado, Marcos Antônio Citrus reticulata; Citrus sinensis; segregation; genetic markers Citrus reticulata; Citrus sinensis; segregação; marcador genético The objective of this work was to evaluate the processes of selection in a citrus hybrid population using segregation analysis of RAPD markers. The segregation of 123 RAPD markers between 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck) was analysed in a F1 progeny of 94 hybrids. Genetic composition, diversity, heterozygosity, differences in chromosomal structure and the presence of deleterious recessive genes are discussed based on the segregation ratios obtained. A high percentage of markers had a skeweness of the 1:1 expected segregation ratio in the F1 population. Many markers showed a 3:1 segregation ratio in both varieties and 1:3 in 'Pêra' sweet orange, probably due to directional selection processes. The distribution analysis of the frequencies of the segregant markers in a hybrid population is a simple method which allows a better understanding of the genetics of citrus group. O objetivo deste trabalho foi avaliar processos de seleção em uma população híbrida de citros, por meio de análise da segregação de marcadores RAPD. A segregação de 123 marcadores RAPD entre tangerina 'Cravo' (Citrus reticulata Blanco) e laranja 'Pêra' (C. sinensis (L.) Osbeck) foi analisada em uma progênie F1 composta por 94 híbridos. Composição genética, diversidade, heterozigosidade, diferenças estruturais dos cromossomos e presença de genes deletérios recessivos nos parentais foram discutidas com base nos tipos de segregação obtidos. Uma porcentagem elevada de marcadores com segregação distorcida em relação à proporção 1:1 esperada foi obtida na população F1. Muitos marcadores apresentaram segregação 3:1 em ambas as variedades e 1:3 em laranja 'Pêra' provavelmente decorrente de um processo de seleção direcional. A análise de distribuição da freqüência dos marcadores segregantes na população híbrida é um método simples que possibilita uma melhor compreensão da genética do grupo citros. Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira 2003-11-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://seer.sct.embrapa.br/index.php/pab/article/view/6703 Pesquisa Agropecuaria Brasileira; v.38, n.11, nov. 2003; 1297-1302 Pesquisa Agropecuária Brasileira; v.38, n.11, nov. 2003; 1297-1302 1678-3921 0100-104x eng https://seer.sct.embrapa.br/index.php/pab/article/view/6703/3760 |
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| author |
Oliveira, Roberto Pedroso de Vildoso, Carlos Ivan Aguilar Machado, Marcos Antônio |
| spellingShingle |
Oliveira, Roberto Pedroso de Vildoso, Carlos Ivan Aguilar Machado, Marcos Antônio Selection processes in a citrus hybrid population using RAPD markers |
| author_facet |
Oliveira, Roberto Pedroso de Vildoso, Carlos Ivan Aguilar Machado, Marcos Antônio |
| author_sort |
Oliveira, Roberto Pedroso de |
| title |
Selection processes in a citrus hybrid population using RAPD markers |
| title_short |
Selection processes in a citrus hybrid population using RAPD markers |
| title_full |
Selection processes in a citrus hybrid population using RAPD markers |
| title_fullStr |
Selection processes in a citrus hybrid population using RAPD markers |
| title_full_unstemmed |
Selection processes in a citrus hybrid population using RAPD markers |
| title_sort |
selection processes in a citrus hybrid population using rapd markers |
| description |
The objective of this work was to evaluate the processes of selection in a citrus hybrid population using segregation analysis of RAPD markers. The segregation of 123 RAPD markers between 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck) was analysed in a F1 progeny of 94 hybrids. Genetic composition, diversity, heterozygosity, differences in chromosomal structure and the presence of deleterious recessive genes are discussed based on the segregation ratios obtained. A high percentage of markers had a skeweness of the 1:1 expected segregation ratio in the F1 population. Many markers showed a 3:1 segregation ratio in both varieties and 1:3 in 'Pêra' sweet orange, probably due to directional selection processes. The distribution analysis of the frequencies of the segregant markers in a hybrid population is a simple method which allows a better understanding of the genetics of citrus group. |
| publisher |
Pesquisa Agropecuaria Brasileira |
| publishDate |
2003 |
| url |
https://seer.sct.embrapa.br/index.php/pab/article/view/6703 |
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