Silicon and agar on in vitro development of cockscomb (Amaranthaceae)
The objective of this work was to evaluate the effect of silicon and agar concentrations on cockscomb (Celosia cristata) (Amaranthaceae) development in vitro. Phytotechnical, anatomical, and ultrastructural analyses were carried out in a completely randomized design, in a 4x3 factorial arrangment, with four silicic acid concentrations (0.0, 0.5, 1.0, and 1.5 g L-1) and three agar concentrations (5.5, 8.0, and 10.5 g L-1), totaling 12 treatments with seven replicates. Celosia cristata explants were inoculated in vitro in a Murashige & Skoog (MS) culture medium, with 30 g L-1 sucrose. The analyses were conducted 30 days after inoculation. Phytotechnical traits improve with agar concentration of 8.0 g L-1, and the association of this concentration with 1.0 g L-1 silicon also contributes to the improvement of anatomical attributes, especially of leaf blade width and thickness of the palisade and spongy parenchyma, which contributes to the development of C. cristata. None of the evaluated combinations of agar and silicon contributes to Si deposition on the foliar epidermis of this ornamental species.
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Pesquisa Agropecuaria Brasileira
2018
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rev-pab-br-article-253702018-02-22T15:14:27Z Silicon and agar on in vitro development of cockscomb (Amaranthaceae) Silício e ágar no desenvolvimento in vitro de crista-de-galo (Amaranthaceae) Assis, Franscinely Aparecida de Souza, Genaina Aparecida de Dias, Gabrielen de Maria Gomes Assis, Gleice Aparecida de Rodrigues, Filipe Almendagna Pasqual, Moacir Costa, Bárbara Nogueira Souza Carvalho, Fabio Janoni Celosia cristata; gelling agent; micropropagation; ornamental plant; silicic acid Celosia cristata; agente geleificante; micropropagação; planta ornamental; ácido silícico The objective of this work was to evaluate the effect of silicon and agar concentrations on cockscomb (Celosia cristata) (Amaranthaceae) development in vitro. Phytotechnical, anatomical, and ultrastructural analyses were carried out in a completely randomized design, in a 4x3 factorial arrangment, with four silicic acid concentrations (0.0, 0.5, 1.0, and 1.5 g L-1) and three agar concentrations (5.5, 8.0, and 10.5 g L-1), totaling 12 treatments with seven replicates. Celosia cristata explants were inoculated in vitro in a Murashige & Skoog (MS) culture medium, with 30 g L-1 sucrose. The analyses were conducted 30 days after inoculation. Phytotechnical traits improve with agar concentration of 8.0 g L-1, and the association of this concentration with 1.0 g L-1 silicon also contributes to the improvement of anatomical attributes, especially of leaf blade width and thickness of the palisade and spongy parenchyma, which contributes to the development of C. cristata. None of the evaluated combinations of agar and silicon contributes to Si deposition on the foliar epidermis of this ornamental species. O objetivo deste trabalho foi avaliar o efeito de concentrações de silício e ágar no desenvolvimento in vitro de crista-de-galo (Celosia cristata) (Amaranthaceae). Análises fitotécnicas, anatômicas e ultraestruturais foram conduzidas em delineamento inteiramente casualizado, em arranjo fatorial 4x3, com quatro concentrações de ácido silícico (0,0, 0,5, 1,0 e 1,5 g L-1) e três de ágar (5,5, 8,0 e 10,5 g L-1), no total de 12 tratamentos com sete repetições. Explantes de C. cristata foram inoculados in vitro em meio de cultura Murashige & Skoog (MS ), com 30 g L-1 de sacarose. As análises foram realizadas 30 dias após a inoculação. As características fitotécnicas melhoram com a concentração de ágar a 8,0 g L-1, e a associação dessa concentração com 1,0 g L-1 de silício contribui ainda para a melhoria dos atributos anatômicos, principalmente a largura do limbo foliar e a espessura dos parênquimas paliçádico e esponjoso, o que colabora para o desenvolvimento de C. cristata. Nenhuma das combinações avaliadas de silício e ágar contribui para deposição de Si na epiderme foliar dessa espécie ornamental. Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) 2018-02-16 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://seer.sct.embrapa.br/index.php/pab/article/view/25370 Pesquisa Agropecuaria Brasileira; v.53, n.1, jan. 2018; 30-41 Pesquisa Agropecuária Brasileira; v.53, n.1, jan. 2018; 30-41 1678-3921 0100-104x eng https://seer.sct.embrapa.br/index.php/pab/article/view/25370/14103 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/25370/17132 Direitos autorais 2018 Pesquisa Agropecuária Brasileira |
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Assis, Franscinely Aparecida de Souza, Genaina Aparecida de Dias, Gabrielen de Maria Gomes Assis, Gleice Aparecida de Rodrigues, Filipe Almendagna Pasqual, Moacir Costa, Bárbara Nogueira Souza Carvalho, Fabio Janoni |
| spellingShingle |
Assis, Franscinely Aparecida de Souza, Genaina Aparecida de Dias, Gabrielen de Maria Gomes Assis, Gleice Aparecida de Rodrigues, Filipe Almendagna Pasqual, Moacir Costa, Bárbara Nogueira Souza Carvalho, Fabio Janoni Silicon and agar on in vitro development of cockscomb (Amaranthaceae) |
| author_facet |
Assis, Franscinely Aparecida de Souza, Genaina Aparecida de Dias, Gabrielen de Maria Gomes Assis, Gleice Aparecida de Rodrigues, Filipe Almendagna Pasqual, Moacir Costa, Bárbara Nogueira Souza Carvalho, Fabio Janoni |
| author_sort |
Assis, Franscinely Aparecida de |
| title |
Silicon and agar on in vitro development of cockscomb (Amaranthaceae) |
| title_short |
Silicon and agar on in vitro development of cockscomb (Amaranthaceae) |
| title_full |
Silicon and agar on in vitro development of cockscomb (Amaranthaceae) |
| title_fullStr |
Silicon and agar on in vitro development of cockscomb (Amaranthaceae) |
| title_full_unstemmed |
Silicon and agar on in vitro development of cockscomb (Amaranthaceae) |
| title_sort |
silicon and agar on in vitro development of cockscomb (amaranthaceae) |
| description |
The objective of this work was to evaluate the effect of silicon and agar concentrations on cockscomb (Celosia cristata) (Amaranthaceae) development in vitro. Phytotechnical, anatomical, and ultrastructural analyses were carried out in a completely randomized design, in a 4x3 factorial arrangment, with four silicic acid concentrations (0.0, 0.5, 1.0, and 1.5 g L-1) and three agar concentrations (5.5, 8.0, and 10.5 g L-1), totaling 12 treatments with seven replicates. Celosia cristata explants were inoculated in vitro in a Murashige & Skoog (MS) culture medium, with 30 g L-1 sucrose. The analyses were conducted 30 days after inoculation. Phytotechnical traits improve with agar concentration of 8.0 g L-1, and the association of this concentration with 1.0 g L-1 silicon also contributes to the improvement of anatomical attributes, especially of leaf blade width and thickness of the palisade and spongy parenchyma, which contributes to the development of C. cristata. None of the evaluated combinations of agar and silicon contributes to Si deposition on the foliar epidermis of this ornamental species. |
| publisher |
Pesquisa Agropecuaria Brasileira |
| publishDate |
2018 |
| url |
https://seer.sct.embrapa.br/index.php/pab/article/view/25370 |
| work_keys_str_mv |
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| _version_ |
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