Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny
The objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations.
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Pesquisa Agropecuaria Brasileira
2010
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rev-pab-br-article-22002012-06-17T11:53:41Z Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny Genotipagem do endosperma como estratégia para diferenciar a origem de alelos em progênies heterozigóticas de milho Martins, Francielle Alline Carneiro, Pedro Crescêncio Souza Cruz, Cosme Damião Carneiro, José Eustáquio de Souza Guimarães, Claudia Teixeira Zea mays; allelic origin; heterozygote; natural populations; polygenes; semiquantitative PCR Zea mays; origem alélica; heterozigoto; populações naturais; poligenes; PCR semiquantitativa The objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations. O objetivo deste trabalho foi distinguir a origem de alelos em progênies heterozigóticas usando reação em cadeia da polimerase (PCR) semiquantitativa em endosperma de milho. Endospermas derivados de híbridos simples diretos e recíprocos entre as linhagens de milho L3 e L1113-01 foram genotipados pela metodologia de PCR semiquantitativa (PCR-SQ) com uso de primers microssatélites fluorescentes. Os produtos de amplificação foram avaliados por meio da razão de intensidade de fluorescência (RIF), calculada entre os valores de intensidade dos picos correspondentes aos alelos derivados de cada genitor. Com base no contraste estatisticamente significativo dos valores médios das RIF entre os híbridos simples direto e recíproco, foi possível distinguir o número de alelos recebidos de cada genitor e, finalmente, determinar a origem dos alelos de cada híbrido. Assim, a genotipagem de endosperma utilizando PCR-SQ é uma estratégia promissora no mapeamento de QTLs em populações exogâmicas de milho. Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira FAPEMIG E CNPq 2010-12-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://seer.sct.embrapa.br/index.php/pab/article/view/2200 Pesquisa Agropecuaria Brasileira; v.44, n.10, out. 2009; 1291-1296 Pesquisa Agropecuária Brasileira; v.44, n.10, out. 2009; 1291-1296 1678-3921 0100-104x por https://seer.sct.embrapa.br/index.php/pab/article/view/2200/5842 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2200/1654 |
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Martins, Francielle Alline Carneiro, Pedro Crescêncio Souza Cruz, Cosme Damião Carneiro, José Eustáquio de Souza Guimarães, Claudia Teixeira |
| spellingShingle |
Martins, Francielle Alline Carneiro, Pedro Crescêncio Souza Cruz, Cosme Damião Carneiro, José Eustáquio de Souza Guimarães, Claudia Teixeira Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny |
| author_facet |
Martins, Francielle Alline Carneiro, Pedro Crescêncio Souza Cruz, Cosme Damião Carneiro, José Eustáquio de Souza Guimarães, Claudia Teixeira |
| author_sort |
Martins, Francielle Alline |
| title |
Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny |
| title_short |
Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny |
| title_full |
Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny |
| title_fullStr |
Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny |
| title_full_unstemmed |
Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny |
| title_sort |
endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny |
| description |
The objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations. |
| publisher |
Pesquisa Agropecuaria Brasileira |
| publishDate |
2010 |
| url |
https://seer.sct.embrapa.br/index.php/pab/article/view/2200 |
| work_keys_str_mv |
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