Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions: Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions
Tillansia usneoides L., commonly known as “Spanish moss” is a plant with an epiphytic growth habit, it is found within the atmospheric type bromeliads that comprise small plants whose leaves are densely covered with trichomes; They are slow to reproduce and slow to grow, often obtained from illegal extraction from the wild. Therefore, the objective of this study is to observe the in vitro propagation of Tillandsia usneoides L., 12 pregerminative treatments were evaluated: (T1) room temperature, (T2) soaking for 12 hours, (T3) TDZ 0.22 mg·L⁻¹, ( T4 and T5) potassium nitrate 0.1% and 0.2%, (T6 and T7) gibberellins 50 mg·L⁻¹ and 150 mg·L⁻¹, (T8 and T9) hydrogen peroxide 10% and 20%, (T10 and T11 ) chitosan 250 mg·L⁻¹ and 500 mg·L⁻¹ and (T12) 2,4-D 0.22 mg·L⁻¹. The seeds were established in deionized water and Deiman® agar, 5.5 g L⁻¹, pH 5.5. The analysis of variance (p ≤ 0.05%) did not show significant statistical differences. The treatment with the highest number of seeds that started the germination process and the highest number of plants was gibberellin 50 mg·L⁻¹. The greatest contamination was observed in the 0.1% potassium nitrate treatment.
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UNIVERSIDAD DE GUADALAJARA
2024
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rev-ecucba-mx-article3372024-05-02T04:51:59Z Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions: Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions Evaluación de tratamientos pregerminativos de heno (Tillandsia usneoides L.) en condiciones in vitro: Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions Luján-Santiago, Virgen Valdez-Hernández, Edna Fabiola Juárez-Hernández, Ma. De Jesús Sánchez-Carrillo, Román Vázquez-Flores , Xareni Propagación latencia bromelia germinación plántula Tillansia usneoides L., commonly known as “Spanish moss” is a plant with an epiphytic growth habit, it is found within the atmospheric type bromeliads that comprise small plants whose leaves are densely covered with trichomes; They are slow to reproduce and slow to grow, often obtained from illegal extraction from the wild. Therefore, the objective of this study is to observe the in vitro propagation of Tillandsia usneoides L., 12 pregerminative treatments were evaluated: (T1) room temperature, (T2) soaking for 12 hours, (T3) TDZ 0.22 mg·L⁻¹, ( T4 and T5) potassium nitrate 0.1% and 0.2%, (T6 and T7) gibberellins 50 mg·L⁻¹ and 150 mg·L⁻¹, (T8 and T9) hydrogen peroxide 10% and 20%, (T10 and T11 ) chitosan 250 mg·L⁻¹ and 500 mg·L⁻¹ and (T12) 2,4-D 0.22 mg·L⁻¹. The seeds were established in deionized water and Deiman® agar, 5.5 g L⁻¹, pH 5.5. The analysis of variance (p ≤ 0.05%) did not show significant statistical differences. The treatment with the highest number of seeds that started the germination process and the highest number of plants was gibberellin 50 mg·L⁻¹. The greatest contamination was observed in the 0.1% potassium nitrate treatment. Tillansia usneoides L., conocida comúnmente como “heno” es una planta con hábito de crecimiento epífito, se encuentra dentro de las bromelias de tipo atmosférico que comprende las plantas pequeñas cuyas hojas están densamente cubiertas de tricomas; son de lenta reproducción y lento crecimiento, a menudo se obtiene de extracción ilegal del medio silvestre. Por lo que el objetivo del presente es observar la propagación in vitro de Tillandsia usneoides L., se evaluaron 12 tratamientos pregerminativos: (T1) temperatura ambiente, (T2) remojo 12 horas, (T3) TDZ 0.22 mg·L⁻¹, (T4 y T5) nitrato de potasio 0.1 % y 0.2 %, (T6 y T7) giberelinas 50 mg·L⁻¹ y 150 mg·L⁻¹, (T8 y T9) agua oxigenada 10 % y 20 %, (T10 y T11) chitosan 250 mg·L⁻¹ y 500 mg·L⁻¹ y (T12) 2,4-D 0.22 mg·L⁻¹. Las semillas fueron establecidas en agua desionizada y agar-agar Deiman®, 5.5 g·L⁻¹, pH a 5.5. El análisis de varianza (p ≤ 0.05 %) no mostró diferencias estadísticas significativas. El tratamiento con mayor número de semillas que iniciaron el proceso de germinación y mayor número de plantas fueron las giberelinas 50 mg·L⁻¹. La mayor contaminación se observó en el tratamiento nitrato de potasio 0.1 %. UNIVERSIDAD DE GUADALAJARA 2024-01-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://e-cucba.cucba.udg.mx/index.php/e-Cucba/article/view/337 10.32870/e-cucba.vi21.337 e-CUCBA; Núm. 21 (11): e-CUCBA | Enero - Junio de 2024; 189 - 194 2448-5225 10.32870/e-cucba.vi21 spa http://e-cucba.cucba.udg.mx/index.php/e-Cucba/article/view/337/345 10.32870/e-cucba.vi21.337.g345 Derechos de autor 2024 Virgen Luján-Santiago, Edna Fabiola Valdez-Hernández, Ma. De Jesús Juárez-Hernández, Román Sánchez-Carrillo, Xareni Vázquez-Flores https://creativecommons.org/licenses/by-nc-nd/4.0 |
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Luján-Santiago, Virgen Valdez-Hernández, Edna Fabiola Juárez-Hernández, Ma. De Jesús Sánchez-Carrillo, Román Vázquez-Flores , Xareni |
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Luján-Santiago, Virgen Valdez-Hernández, Edna Fabiola Juárez-Hernández, Ma. De Jesús Sánchez-Carrillo, Román Vázquez-Flores , Xareni Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions: Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions |
author_facet |
Luján-Santiago, Virgen Valdez-Hernández, Edna Fabiola Juárez-Hernández, Ma. De Jesús Sánchez-Carrillo, Román Vázquez-Flores , Xareni |
author_sort |
Luján-Santiago, Virgen |
title |
Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions: Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions |
title_short |
Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions: Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions |
title_full |
Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions: Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions |
title_fullStr |
Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions: Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions |
title_full_unstemmed |
Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions: Evaluation of pregerminative treatments of hay (Tillandsia usneoides L.) under in vitro conditions |
title_sort |
evaluation of pregerminative treatments of hay (tillandsia usneoides l.) under in vitro conditions: evaluation of pregerminative treatments of hay (tillandsia usneoides l.) under in vitro conditions |
description |
Tillansia usneoides L., commonly known as “Spanish moss” is a plant with an epiphytic growth habit, it is found within the atmospheric type bromeliads that comprise small plants whose leaves are densely covered with trichomes; They are slow to reproduce and slow to grow, often obtained from illegal extraction from the wild. Therefore, the objective of this study is to observe the in vitro propagation of Tillandsia usneoides L., 12 pregerminative treatments were evaluated: (T1) room temperature, (T2) soaking for 12 hours, (T3) TDZ 0.22 mg·L⁻¹, ( T4 and T5) potassium nitrate 0.1% and 0.2%, (T6 and T7) gibberellins 50 mg·L⁻¹ and 150 mg·L⁻¹, (T8 and T9) hydrogen peroxide 10% and 20%, (T10 and T11 ) chitosan 250 mg·L⁻¹ and 500 mg·L⁻¹ and (T12) 2,4-D 0.22 mg·L⁻¹. The seeds were established in deionized water and Deiman® agar, 5.5 g L⁻¹, pH 5.5. The analysis of variance (p ≤ 0.05%) did not show significant statistical differences. The treatment with the highest number of seeds that started the germination process and the highest number of plants was gibberellin 50 mg·L⁻¹. The greatest contamination was observed in the 0.1% potassium nitrate treatment. |
publisher |
UNIVERSIDAD DE GUADALAJARA |
publishDate |
2024 |
url |
http://e-cucba.cucba.udg.mx/index.php/e-Cucba/article/view/337 |
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