Genetic resistance to coffee berry disease on coffee varieties cultivated in Colombia
Coffee Berry disease (CDB), an anthracnose caused by the fungus Colletotrichum kahawae subsp. Kahawae, has been registered only in Africa and it can cause up to 80% of crop losses. Cenicafé works on the development of varieties resistant to the main coffee diseases, even in absence of the pathogens in Colombia; such studies use Timor Hybrid (HT) as the main source of resistance against coffee leaf rust (CLR) and CBD. The objective of this study was to screen the presence of the Ck-1 gene, resistant to CBD, on the main coffee varieties cultivated in Colombia in order to establish the correlation between the presence of Ck-1 and the response to hypocotyl inoculation, and to explore the genomic basis of the resistance. Molecular markers linked to Ck-1 were located at the Coffea canephora chromosome 1, a genomic region with fifteen disease resistance genes. Markers for Ck-1 were found in all the breeding lines derived from TH-1343 and absent in Typica, Bourbon, and Caturra varieties and in lines derived from TH-832/1. There was no correlation between the allelic forms of CBD resistance and the resistance of the genotype measured by the hypocotyl inoculation test. The high frequency of allelic forms associated with CDB resistance in materials selected mainly for CLR resistance suggests co-segregation of resistance genes for both diseases. The strategy of multi-line varieties developed by Cenicafé, which currently corresponds to 80% of the coffee planted in Colombia, protects the crop population against the eventual arrival of the pathogen in Colombia.
| Main Authors: | , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Cenicafé
2020
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| Online Access: | https://publicaciones.cenicafe.org/index.php/cenicafe/article/view/3 |
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| Summary: | Coffee Berry disease (CDB), an anthracnose caused by the fungus Colletotrichum kahawae subsp. Kahawae, has been registered only in Africa and it can cause up to 80% of crop losses. Cenicafé works on the development of varieties resistant to the main coffee diseases, even in absence of the pathogens in Colombia; such studies use Timor Hybrid (HT) as the main source of resistance against coffee leaf rust (CLR) and CBD. The objective of this study was to screen the presence of the Ck-1 gene, resistant to CBD, on the main coffee varieties cultivated in Colombia in order to establish the correlation between the presence of Ck-1 and the response to hypocotyl inoculation, and to explore the genomic basis of the resistance. Molecular markers linked to Ck-1 were located at the Coffea canephora chromosome 1, a genomic region with fifteen disease resistance genes. Markers for Ck-1 were found in all the breeding lines derived from TH-1343 and absent in Typica, Bourbon, and Caturra varieties and in lines derived from TH-832/1. There was no correlation between the allelic forms of CBD resistance and the resistance of the genotype measured by the hypocotyl inoculation test. The high frequency of allelic forms associated with CDB resistance in materials selected mainly for CLR resistance suggests co-segregation of resistance genes for both diseases. The strategy of multi-line varieties developed by Cenicafé, which currently corresponds to 80% of the coffee planted in Colombia, protects the crop population against the eventual arrival of the pathogen in Colombia. |
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