GENETIC Diaphorina citri DIVERSITY ON CITRUS CROPS OF THE VALLE DEL CAUCA AND QUINDÍO (COLOMBIA)
The Asiatic psyllid Diaphorina citri (Hemiptera: Psyllidae) is the main vector of Candidatus liberibacter, which causes the Huanglongbing HLB disease, known for devastating citrus in the world but not yet reported in Colombia. The genetic variability of the D. citri population was studied through sequencing the COI mitochondrial gene as molecular marker. Adults were collected in citrus producing zones of the Colombian Valle del Cauca and Quindío. Amplification was performed with two pairs of specific primers for Hemiptera. The PCR products were sequenced at Macrogen-Korea, obtaining a total of 124 sequences. For the bioinformatic analysis, the Vector NTI 11.5, Harlequin V 3.5, MEGA 5 and MAFFT 6 programs were used. The molecular diversity indices between populations were similar, revealing a common origin and a recent split of the populations excluding a significant genetic differentiation associated to variations of the bacterium, however the haplotype diversity index was higher than the nucleotide diversity index. The latter one showed a low number of polymorphic sites, indicating that the D. citri populations are expanding. The study of the vector’s genetic variability is a tool for the prediction of likely scenarios for the spread of diseases.
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Universidad del Cauca -Facultad de ciencias Agrarias
2014
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Moncayo Donoso, Miguel Anguel Almanza Pinzon, Martha Isabel Caicedo Vallejo, Ana Milena Muñoz Florez, Jaime Eduardo Montoya Lerma, James Carabali Muñoz, Arturo |
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Moncayo Donoso, Miguel Anguel Almanza Pinzon, Martha Isabel Caicedo Vallejo, Ana Milena Muñoz Florez, Jaime Eduardo Montoya Lerma, James Carabali Muñoz, Arturo GENETIC Diaphorina citri DIVERSITY ON CITRUS CROPS OF THE VALLE DEL CAUCA AND QUINDÍO (COLOMBIA) |
| author_facet |
Moncayo Donoso, Miguel Anguel Almanza Pinzon, Martha Isabel Caicedo Vallejo, Ana Milena Muñoz Florez, Jaime Eduardo Montoya Lerma, James Carabali Muñoz, Arturo |
| author_sort |
Moncayo Donoso, Miguel Anguel |
| title |
GENETIC Diaphorina citri DIVERSITY ON CITRUS CROPS OF THE VALLE DEL CAUCA AND QUINDÍO (COLOMBIA) |
| title_short |
GENETIC Diaphorina citri DIVERSITY ON CITRUS CROPS OF THE VALLE DEL CAUCA AND QUINDÍO (COLOMBIA) |
| title_full |
GENETIC Diaphorina citri DIVERSITY ON CITRUS CROPS OF THE VALLE DEL CAUCA AND QUINDÍO (COLOMBIA) |
| title_fullStr |
GENETIC Diaphorina citri DIVERSITY ON CITRUS CROPS OF THE VALLE DEL CAUCA AND QUINDÍO (COLOMBIA) |
| title_full_unstemmed |
GENETIC Diaphorina citri DIVERSITY ON CITRUS CROPS OF THE VALLE DEL CAUCA AND QUINDÍO (COLOMBIA) |
| title_sort |
genetic diaphorina citri diversity on citrus crops of the valle del cauca and quindío (colombia) |
| description |
The Asiatic psyllid Diaphorina citri (Hemiptera: Psyllidae) is the main vector of Candidatus liberibacter, which causes the Huanglongbing HLB disease, known for devastating citrus in the world but not yet reported in Colombia. The genetic variability of the D. citri population was studied through sequencing the COI mitochondrial gene as molecular marker. Adults were collected in citrus producing zones of the Colombian Valle del Cauca and Quindío. Amplification was performed with two pairs of specific primers for Hemiptera. The PCR products were sequenced at Macrogen-Korea, obtaining a total of 124 sequences. For the bioinformatic analysis, the Vector NTI 11.5, Harlequin V 3.5, MEGA 5 and MAFFT 6 programs were used. The molecular diversity indices between populations were similar, revealing a common origin and a recent split of the populations excluding a significant genetic differentiation associated to variations of the bacterium, however the haplotype diversity index was higher than the nucleotide diversity index. The latter one showed a low number of polymorphic sites, indicating that the D. citri populations are expanding. The study of the vector’s genetic variability is a tool for the prediction of likely scenarios for the spread of diseases. |
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Universidad del Cauca -Facultad de ciencias Agrarias |
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2014 |
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https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/351 |
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rev-bsaa-co-article-3512018-11-02T21:14:51Z GENETIC Diaphorina citri DIVERSITY ON CITRUS CROPS OF THE VALLE DEL CAUCA AND QUINDÍO (COLOMBIA) Diversidad genética de diaphorina citri en cultivos cítricos del valle del cauca y Quindío (Colombia) Moncayo Donoso, Miguel Anguel Almanza Pinzon, Martha Isabel Caicedo Vallejo, Ana Milena Muñoz Florez, Jaime Eduardo Montoya Lerma, James Carabali Muñoz, Arturo Mitochondrial DNA Citrus Regreening Vector Insects Huanglongbing Asiatic Citrus Psyllid. ADN Mitocondrial Reverdecimiento de los Cítricos Insectos Vectores Huanglongbing Psylido Asiático de los Cítricos. The Asiatic psyllid Diaphorina citri (Hemiptera: Psyllidae) is the main vector of Candidatus liberibacter, which causes the Huanglongbing HLB disease, known for devastating citrus in the world but not yet reported in Colombia. The genetic variability of the D. citri population was studied through sequencing the COI mitochondrial gene as molecular marker. Adults were collected in citrus producing zones of the Colombian Valle del Cauca and Quindío. Amplification was performed with two pairs of specific primers for Hemiptera. The PCR products were sequenced at Macrogen-Korea, obtaining a total of 124 sequences. For the bioinformatic analysis, the Vector NTI 11.5, Harlequin V 3.5, MEGA 5 and MAFFT 6 programs were used. The molecular diversity indices between populations were similar, revealing a common origin and a recent split of the populations excluding a significant genetic differentiation associated to variations of the bacterium, however the haplotype diversity index was higher than the nucleotide diversity index. The latter one showed a low number of polymorphic sites, indicating that the D. citri populations are expanding. The study of the vector’s genetic variability is a tool for the prediction of likely scenarios for the spread of diseases. El psílido asiático Diaphorina citri es el principal vector de la bacteria CandidatusLiberibacter que causa la enfermedad Huanglongbing, considerada devastadoraen la citricultura mundial y aún no reportada en Colombia. La variabilidad genéticapoblacional de D. citri fue estudiada utilizando la técnica de secuenciación del genCOI mitocondrial como marcador molecular. Se realizaron colectas de adultos enzonas productoras de cítricos del Valle del Cauca y el Quindío. La amplificación serealizó con dos parejas de cebadores específicos para hemípteros. Los productosde la PCR fueron secuenciados en Macrogen-Korea obteniéndose un total de 124secuencias. En el análisis bioinformático se utilizaron los programas Vector NTI11.5, Arlequín V 3.5, MEGA 5 y MAFFT 6. Los índices de diversidad molecularfueron similares entre poblaciones, mostrando un mismo origen, separación re-ciente de poblaciones y que no existe diferenciación genética significativaque pueda albergar variantes de la bacteria; sin embargo, el índice de diversidadhaplotípica fue más alto que el de diversidad nucleotídica y esteúltimo índice presentó bajo número de sitios polimórficos, indicando quelas poblaciones de D. citri están en expansión. El estudio de la variabilidadgenética del vector representa una herramienta para predecir escenariosprobables de propagación de la enfermedad. Universidad del Cauca -Facultad de ciencias Agrarias 2014-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/351 Biotechnology in the Agricultural and Agroindustrial Sector; Vol. 12 No. 2 (2014): July to December; 106-114 Biotecnología en el Sector Agropecuario y Agroindustrial; Vol. 12 Núm. 2 (2014): Julio a Diciembre; 106-114 1909-9959 1692-3561 spa https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/351/544 /*ref*/CERMELI, M., MORALES, P., y GODOY, F. Presencia del psílido asiático de los cítricos Diaphorina citri Kuwayama (Hemiptera: Psyllidae) en Venezuela. Boletín Entomológico Venezuela, 15(2), 2000, p. 235-243. /*ref*/REYES, R. Chicharrita (Diaphorina citri) amenaza para la citricultura en el Salvador. San Salvador (El Salvador): Programa Nacional de Frutas del Salvador, 2006, 4 p. /*ref*/LANTERI, A., LOIÁCONO, M.S. y MARGARÍA, C. 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