Evaluation of phosphorus solubilizing endophytic bacteria in coffee, an alternative sustainable

Evaluation of phosphorus solubilizing endophytic bacteria in coffee, an alternative sustainable

Soil fertility is affected by its acidic characteristics, causing limitations in the availability of phosphorus because it is absorbed or precipitated with iron and aluminum. The objective of the work was the search for endophytic bacteria phosphorus solubilizers of coffee variety bourbon and its evaluation in the promotion of growth in the coffee crop. Roots were taken, which were subjected to superficial disinfection, then macerated, the bacteria were isolated and characterized, by phylogenetic allocation (16S RNAr) and evaluation of the effect of inoculation on growth in seedling coffee. The results showed that coffee has phosphorus-solubilizing endophytic bacteria, from which 18 strains were obtained with assignment to Gammaproteobacteria (72 %), Firmicutes (22,3 %) and Betaproteobacteria (5,5 %). The End-F-34 and End-F-5 strains stood out for their ability to solubilize phosphorus, these were used in the seed inoculation test in coffee. it is concluded that the var. bourbon has phosphorus solubilizing microorganisms with endophytic habit and ability to stimulate coffee plants in their growth, these results generate great expectations for the use of the strains as biofertilizer.

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Main Authors: Ramos Cabrera, Efren Venancio, Delgado Espinosa, Zuly Yuliana, Murillo Muñoz, Robinson Andres, Muños Días, Víctor Eduardo, Hoyos garcía, Javier
Format: Digital revista
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Published: Universidad del Cauca -Facultad de ciencias Agrarias 2021
Online Access:https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/1554
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author Ramos Cabrera, Efren Venancio
Delgado Espinosa, Zuly Yuliana
Murillo Muñoz, Robinson Andres
Muños Días, Víctor Eduardo
Hoyos garcía, Javier
spellingShingle Ramos Cabrera, Efren Venancio
Delgado Espinosa, Zuly Yuliana
Murillo Muñoz, Robinson Andres
Muños Días, Víctor Eduardo
Hoyos garcía, Javier
Evaluation of phosphorus solubilizing endophytic bacteria in coffee, an alternative sustainable
author_facet Ramos Cabrera, Efren Venancio
Delgado Espinosa, Zuly Yuliana
Murillo Muñoz, Robinson Andres
Muños Días, Víctor Eduardo
Hoyos garcía, Javier
author_sort Ramos Cabrera, Efren Venancio
title Evaluation of phosphorus solubilizing endophytic bacteria in coffee, an alternative sustainable
title_short Evaluation of phosphorus solubilizing endophytic bacteria in coffee, an alternative sustainable
title_full Evaluation of phosphorus solubilizing endophytic bacteria in coffee, an alternative sustainable
title_fullStr Evaluation of phosphorus solubilizing endophytic bacteria in coffee, an alternative sustainable
title_full_unstemmed Evaluation of phosphorus solubilizing endophytic bacteria in coffee, an alternative sustainable
title_sort evaluation of phosphorus solubilizing endophytic bacteria in coffee, an alternative sustainable
description Soil fertility is affected by its acidic characteristics, causing limitations in the availability of phosphorus because it is absorbed or precipitated with iron and aluminum. The objective of the work was the search for endophytic bacteria phosphorus solubilizers of coffee variety bourbon and its evaluation in the promotion of growth in the coffee crop. Roots were taken, which were subjected to superficial disinfection, then macerated, the bacteria were isolated and characterized, by phylogenetic allocation (16S RNAr) and evaluation of the effect of inoculation on growth in seedling coffee. The results showed that coffee has phosphorus-solubilizing endophytic bacteria, from which 18 strains were obtained with assignment to Gammaproteobacteria (72 %), Firmicutes (22,3 %) and Betaproteobacteria (5,5 %). The End-F-34 and End-F-5 strains stood out for their ability to solubilize phosphorus, these were used in the seed inoculation test in coffee. it is concluded that the var. bourbon has phosphorus solubilizing microorganisms with endophytic habit and ability to stimulate coffee plants in their growth, these results generate great expectations for the use of the strains as biofertilizer.
publisher Universidad del Cauca -Facultad de ciencias Agrarias
publishDate 2021
url https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/1554
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spelling rev-bsaa-co-article-15542022-01-03T21:40:29Z Evaluation of phosphorus solubilizing endophytic bacteria in coffee, an alternative sustainable Evaluación de bacterias endofíticas solubilizadores de fósforo en café, una alternativa sostenible AVALIAÇÃO DE BACTÉRIAS ENFOFÍTICAS SOLUBILIZANTES DE FÓSFORO EM CAFÉ, UMA ALTERNATIVA AGRICULTURA SUSTENTÁVEL. Ramos Cabrera, Efren Venancio Delgado Espinosa, Zuly Yuliana Murillo Muñoz, Robinson Andres Muños Días, Víctor Eduardo Hoyos garcía, Javier PBS PGPR Suelos ácidos Agricultura sustentable Biotecnología Biofertilizante Bacterias endofítica Fósforo Café Filogenética PBS PGPR Acidic soils Sustainable agriculture Biotechnology Biofertilizer Endophytic bacteria phosphorus Coffee Phylogenetics PBS, Solos ácidos, Alternativas biotecnológicas, Inoculantes Soil fertility is affected by its acidic characteristics, causing limitations in the availability of phosphorus because it is absorbed or precipitated with iron and aluminum. The objective of the work was the search for endophytic bacteria phosphorus solubilizers of coffee variety bourbon and its evaluation in the promotion of growth in the coffee crop. Roots were taken, which were subjected to superficial disinfection, then macerated, the bacteria were isolated and characterized, by phylogenetic allocation (16S RNAr) and evaluation of the effect of inoculation on growth in seedling coffee. The results showed that coffee has phosphorus-solubilizing endophytic bacteria, from which 18 strains were obtained with assignment to Gammaproteobacteria (72 %), Firmicutes (22,3 %) and Betaproteobacteria (5,5 %). The End-F-34 and End-F-5 strains stood out for their ability to solubilize phosphorus, these were used in the seed inoculation test in coffee. it is concluded that the var. bourbon has phosphorus solubilizing microorganisms with endophytic habit and ability to stimulate coffee plants in their growth, these results generate great expectations for the use of the strains as biofertilizer. La fertilidad del suelo se ve afectado por sus características ácidas, que causa limitaciones en la disponibilidad del fosforo debido a que se absorbe o precipita con el hierro y el aluminio. El objetivo del trabajo fue la búsqueda de bacterias endofíticas solubilizadoras de fósforo de café variedad bourbon y su evaluación en la promoción de crecimiento en el cultivo de café. Se tomaron raíces, las cuales fueron sometidas a desinfección superficial luego se aislaron y caracterizaron las bacterias, mediante asignación filogenética (16S RNAr), por último, se evaluó el efecto de la inoculación sobre el crecimiento en café en estado de semillero. Los resultados mostraron que el café tiene bacterias endofíticas solubilizadoras de fosforo, de las cuales se obtuvieron 18 cepas con asignación a Gammaproteobacterias (72 %), Firmicutes (22,3 %) y Betaproteobacterias (5,5 %). Las cepas End-F-34 y End-F-5 se destacaron por su capacidad de solubilizar fósforo, éstas se utilizaron en ensayo de inoculación en café en etapa de semillero. Se concluye que el café variedad Borbón tiene microorganismos solubilizadores de fósforo con hábito endofítica y capacidad de estimular las plantas de café en su crecimiento, estos resultados generan grandes expectativas para la utilización de las cepas como biofertilizante. A fertilidade do solo é afetada por suas características ácidas, causando limitações na disponibilidade de fósforo, pois é absorvido ou precipitado com ferro e alumínio. O objetivo do trabalho foi a busca de bactérias endofíticas solubilizantes de fósforo da variedade café borbon e sua avaliação na promoção do crescimento na cultura do café. Foram colhidas raízes, que foram submetidas à desinfecção superficial e depois maceradas, as bactérias foram isoladas e caracterizadas, por alocação filogenética (16S RNAr) e avaliação do efeito da inoculação no crescimento de mudas de café. Os resultados mostraram que o café possui bactérias endofíticas solubilizadoras de fósforo, das quais foram obtidas 18 cepas atribuídas a Gammaproteobacteria (72%), Firmicutes (22,3%) e Betaproteobacteria (5,5%). As cepas End-F-34 e End-F-5 destacaram-se por sua capacidade de solubilizar fósforo, que foram utilizadas no teste de inoculação de sementes no café. conclui-se que o var. O Bourbon possui microrganismos solubilizantes de fósforo com hábito endofítico e capacidade de estimular o crescimento do cafeeiro; esses resultados geram grandes expectativas quanto ao uso das linhagens como biofertilizante Universidad del Cauca -Facultad de ciencias Agrarias 2021-01-23 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/1554 10.18684/bsaa.v19.n2.2021.1554 Biotechnology in the Agricultural and Agroindustrial Sector; Vol. 19 No. 2 (2021): July to December; 94-107 Biotecnología en el Sector Agropecuario y Agroindustrial; Vol. 19 Núm. 2 (2021): Julio a Diciembre; 94-107 1909-9959 1692-3561 spa https://revistas.unicauca.edu.co/index.php/biotecnologia/article/view/1554/1508 /*ref*/AGARWAL, HEENA; DOWARAH, BHASKAR; BARUAH, POOJA-MONI; BORDOLOI, KUNTALA SARMA; KRISHNATREYA, DEBASISH; AGARWALA, NIRAJ. 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