Formation of a foundation herd of Argentine Creole Cattle for milk production. Effect on six loci gene frequencies

Formation of a foundation herd of Argentine Creole Cattle for milk production. Effect on six loci gene frequencies

With the purpose to establish a foundation group to carry out a dairy improvement plan for Argentine Creole cattle, thirty-eight dams and one sire belonging to this breed were selected. In order to evaluate such selection effect on six loci, polymorphisms of b-lactoglobulin, growth hormone, F13A, BoLA-DRB3, BoLA-DYA, and the microsatellite D23S5 were characterized in the selected population (38 animals), as well as in unselected population (78 animals). The characterization of b-lactoglobulin, growth hormone, F13A, BoLA-DRB3,BoLA-DYA alleles was carried out through the techniques of PCR-RFLP, while the D23S5 variants were characterized by PCR-sequencing gel methodology. These results revealed a significant increase of b-lactoglobulin B variant and non significant increase in D23S5 B variant. Whereas no significant difterences between the selected and unselected population were observed for gene frequencies of growth hormone F13A, BoLA-DRB3, BoLA-DYA.The observed differences could be consequence of selection eftect. Family studies would be nessesary to confirm that the observed differencies were consequence of association between these molecular markers with quantitative trait loci (QTLs) for milk production.

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Main Authors: Ripoli, María, Giovambattista, Guillermo, De Lúea, Julio, Dulout, Fernando, Labarta, Fernando, Echenique, José, Sánchez, Marcelo, Carrizo, Edgardo
Format: Digital revista
Language:spa
Published: Facultad de Ciencias Agrarias. Universidad Austral de Chile 2000
Online Access:http://revistas.uach.cl/index.php/agrosur/article/view/3972
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spelling rev-agrosur-cl-article-39722019-10-01T19:54:12Z Formation of a foundation herd of Argentine Creole Cattle for milk production. Effect on six loci gene frequencies FORMACION DE UN PLANTEL BASE DE GANADO BOVINO CRIOLLO ARGENTINO PARA PRODUCCION LECHERA. EFECTO SOBRE LAS FRECUENCIAS GENICAS DE SEIS LOCI Ripoli, María Giovambattista, Guillermo De Lúea, Julio Dulout, Fernando Labarta, Fernando Echenique, José Sánchez, Marcelo Carrizo, Edgardo Ganado criollo selección marcadores genéticos producción de leche Creole cattle Selection Genetics Markers Milk production With the purpose to establish a foundation group to carry out a dairy improvement plan for Argentine Creole cattle, thirty-eight dams and one sire belonging to this breed were selected. In order to evaluate such selection effect on six loci, polymorphisms of b-lactoglobulin, growth hormone, F13A, BoLA-DRB3, BoLA-DYA, and the microsatellite D23S5 were characterized in the selected population (38 animals), as well as in unselected population (78 animals). The characterization of b-lactoglobulin, growth hormone, F13A, BoLA-DRB3,BoLA-DYA alleles was carried out through the techniques of PCR-RFLP, while the D23S5 variants were characterized by PCR-sequencing gel methodology. These results revealed a significant increase of b-lactoglobulin B variant and non significant increase in D23S5 B variant. Whereas no significant difterences between the selected and unselected population were observed for gene frequencies of growth hormone F13A, BoLA-DRB3, BoLA-DYA.The observed differences could be consequence of selection eftect. Family studies would be nessesary to confirm that the observed differencies were consequence of association between these molecular markers with quantitative trait loci (QTLs) for milk production. Con el objetivo de establecer un plantel base de ganado Bovino Criollo Argentino para producción lechera, se seleccionaron treinta y ocho vientres y un toro pertenecientes a esta raza. Para poder evaluar el efecto de la selección sobre las frecuencias génicas, se caracterizaron los polimorfismos presentes en seis loci: b-lactoglobulina, hormona de crecimiento, F13A, BoLA-DRB3, BoLA-DYA, y en el microsatélite D23S5, tanto en la población seleccionada (38 animales), como en el rebaño no seleccionado (78 animales). La caracterización de b-lactoglobulina, hormona de crecimiento, F13A, BoLA-DRB3, BoLA-DYA se llevó a cabo a través de la técnica de PCR-RFLP, mientras que las variantes del microsatélite D23S5 se caracterizaron mediante PCR-electroforesis en geles de secuenciación. Los resultados evidenciaron un aumento significativo de la variante B de b-lactoglobulina, y un incremento no significativo de la variante B del microsatélite D23S5. Por otra parte las frecuencias génicas de los loci hormona de crecimiento, F13A, BoLA-DRB3, BoLA-DYA, no mostraron diferencias significativas entre la población seleccionada y la no seleccionada. Las diferencias observadas podrían ser consecuencia del efecto de la selección. Sin embargo, sería necesario realizar estudios familiares para confirmar que las diferencias observadas serían consecuencia de la asociación entre los marcadores moleculares analizados con loci de caracteres cuantitativos (QTLs) para producción lechera. Facultad de Ciencias Agrarias. Universidad Austral de Chile 2000-01-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://revistas.uach.cl/index.php/agrosur/article/view/3972 10.4206/agrosur.2000.v28n1-09 Agro sur; Vol. 28 Núm. 1 (2000); 105-122 0719-4196 0304-8802 spa http://revistas.uach.cl/index.php/agrosur/article/view/3972/4915 Derechos de autor 2000 Agro sur
institution UACH CL
collection OJS
country Chile
countrycode CL
component Revista
access En linea
databasecode rev-agrosur-cl
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Ciencias Agrarias y Alimentarias de la UACH
language spa
format Digital
author Ripoli, María
Giovambattista, Guillermo
De Lúea, Julio
Dulout, Fernando
Labarta, Fernando
Echenique, José
Sánchez, Marcelo
Carrizo, Edgardo
spellingShingle Ripoli, María
Giovambattista, Guillermo
De Lúea, Julio
Dulout, Fernando
Labarta, Fernando
Echenique, José
Sánchez, Marcelo
Carrizo, Edgardo
Formation of a foundation herd of Argentine Creole Cattle for milk production. Effect on six loci gene frequencies
author_facet Ripoli, María
Giovambattista, Guillermo
De Lúea, Julio
Dulout, Fernando
Labarta, Fernando
Echenique, José
Sánchez, Marcelo
Carrizo, Edgardo
author_sort Ripoli, María
title Formation of a foundation herd of Argentine Creole Cattle for milk production. Effect on six loci gene frequencies
title_short Formation of a foundation herd of Argentine Creole Cattle for milk production. Effect on six loci gene frequencies
title_full Formation of a foundation herd of Argentine Creole Cattle for milk production. Effect on six loci gene frequencies
title_fullStr Formation of a foundation herd of Argentine Creole Cattle for milk production. Effect on six loci gene frequencies
title_full_unstemmed Formation of a foundation herd of Argentine Creole Cattle for milk production. Effect on six loci gene frequencies
title_sort formation of a foundation herd of argentine creole cattle for milk production. effect on six loci gene frequencies
description With the purpose to establish a foundation group to carry out a dairy improvement plan for Argentine Creole cattle, thirty-eight dams and one sire belonging to this breed were selected. In order to evaluate such selection effect on six loci, polymorphisms of b-lactoglobulin, growth hormone, F13A, BoLA-DRB3, BoLA-DYA, and the microsatellite D23S5 were characterized in the selected population (38 animals), as well as in unselected population (78 animals). The characterization of b-lactoglobulin, growth hormone, F13A, BoLA-DRB3,BoLA-DYA alleles was carried out through the techniques of PCR-RFLP, while the D23S5 variants were characterized by PCR-sequencing gel methodology. These results revealed a significant increase of b-lactoglobulin B variant and non significant increase in D23S5 B variant. Whereas no significant difterences between the selected and unselected population were observed for gene frequencies of growth hormone F13A, BoLA-DRB3, BoLA-DYA.The observed differences could be consequence of selection eftect. Family studies would be nessesary to confirm that the observed differencies were consequence of association between these molecular markers with quantitative trait loci (QTLs) for milk production.
publisher Facultad de Ciencias Agrarias. Universidad Austral de Chile
publishDate 2000
url http://revistas.uach.cl/index.php/agrosur/article/view/3972
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