Bacillus cereus endophytic bacterial plant growth promoter
Lippia origanoides plants have been widely studied for their antimicrobial and antifungal effects of various extracts and essential oils against a large number of pathogenic microorganisms. However, to date there is little record of the diversity of endophytic bacteria associated with this plant species. The objective of the work was to evaluate in vitro the capacity to promote plant growth of endophytic bacteria of Lippia origanoides in the municipality of Sincelejo-Sucre, Colombia. In this study morphotypes were isolated and population density (CFU/ g of tissue) was determined; Qualitative plant growth tests were performed and molecularly identified. The results show that there were significant differences for population density of endophytic bacteria with respect to the type of tissue, with higher values at the root (2.0 x 1010 / g root), followed by the stem (1.3 x 1010 / g stem) and on the sheets (9.2 x 109 / g sheet). A total of 20 endophytic bacteria were isolated, showed phosphate solubilizing capacity, fixing nitrogen, siderophores production and ACC deaminase, corresponding to the TLO5 and RLO4 morphotypes such as Bacillus cereus endophytic bacteria associated with Lippia origanoides plants, which could become a possible resource biological to be used in the promotion of plant growth in these plants producing essential oils.
Main Authors: | , , |
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Format: | Digital revista |
Language: | spa |
Published: |
Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología
2020
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Online Access: | https://revistas.unal.edu.co/index.php/biotecnologia/article/view/81723 |
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Summary: | Lippia origanoides plants have been widely studied for their antimicrobial and antifungal effects of various extracts and essential oils against a large number of pathogenic microorganisms. However, to date there is little record of the diversity of endophytic bacteria associated with this plant species. The objective of the work was to evaluate in vitro the capacity to promote plant growth of endophytic bacteria of Lippia origanoides in the municipality of Sincelejo-Sucre, Colombia. In this study morphotypes were isolated and population density (CFU/ g of tissue) was determined; Qualitative plant growth tests were performed and molecularly identified. The results show that there were significant differences for population density of endophytic bacteria with respect to the type of tissue, with higher values at the root (2.0 x 1010 / g root), followed by the stem (1.3 x 1010 / g stem) and on the sheets (9.2 x 109 / g sheet). A total of 20 endophytic bacteria were isolated, showed phosphate solubilizing capacity, fixing nitrogen, siderophores production and ACC deaminase, corresponding to the TLO5 and RLO4 morphotypes such as Bacillus cereus endophytic bacteria associated with Lippia origanoides plants, which could become a possible resource biological to be used in the promotion of plant growth in these plants producing essential oils. |
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