Identification of polymorphisms in the Cassava RXAM1 Gene and the association with Cassava Bacterial Blight Resistance

Identification of polymorphisms in the Cassava RXAM1 Gene and the association with Cassava Bacterial Blight Resistance

Cassava (Manihot esculenta Crantz) is an essential crop in tropical regions providing food for about one billion people around the world. The most important bacterial disease is cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam). Recently, a resistance gene called RXAM1 was identified, which codes for a protein that has an extracellular LRR (Leucine-Rich Repeat) domain and a cytoplasmic STK (Serine Threonine Kinase) domain. RXAM1 colocalizes with a QTL explaining 13 % of the resistance to the CIO136 strain of Xam. In this work, the response to infection with the strain XamCIO136 in ten different varieties of cassava was evaluated, which allowed the classification of the cassava varieties TMS60444, SG10735, MCOL1522, MCOL1505 and MCOL2215 as susceptible, while CM6438-14, CM523-7, and MBRA902 were cataloged as resistant. Also, SNPs (Single Nucleotide Polymorphism) polymorphisms were identified in the RXAM1 gene in the same group of cassava varieties. SG10735, CM6438-14, TMS6044, and MBRA685 presented the highest level of polymorphisms, while the varieties CM523-7, CM2177-2 and MCOL1522 were less polymorphic for this gene. The statistical analyses did not allow to identify a significant association between the phenotype and the identified polymorphisms. This study represents a first effort towards the establishment of associating between allelic variants and the cassava response to bacterial blight.

Saved in:
Bibliographic Details
Main Authors: Barrera, Andrea del Pilar, Soto-Sedano, Johana, López Carrascal, Camilo Ernesto
Format: Digital revista
Language:spa
Published: Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología 2020
Online Access:https://revistas.unal.edu.co/index.php/actabiol/article/view/77564
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:www.revistas.unal.edu.co:article-77564
record_format ojs
spelling oai:www.revistas.unal.edu.co:article-775642020-04-30T17:05:08Z Identification of polymorphisms in the Cassava RXAM1 Gene and the association with Cassava Bacterial Blight Resistance Identificación de polimorfismos en el gen RXAM1 de yuca y su asociación con la resistencia a la bacteriosis vascular Barrera, Andrea del Pilar Soto-Sedano, Johana López Carrascal, Camilo Ernesto Allele diversity receptor symptoms in plants Alelo diversidad receptor síntomas de plantas Cassava (Manihot esculenta Crantz) is an essential crop in tropical regions providing food for about one billion people around the world. The most important bacterial disease is cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam). Recently, a resistance gene called RXAM1 was identified, which codes for a protein that has an extracellular LRR (Leucine-Rich Repeat) domain and a cytoplasmic STK (Serine Threonine Kinase) domain. RXAM1 colocalizes with a QTL explaining 13 % of the resistance to the CIO136 strain of Xam. In this work, the response to infection with the strain XamCIO136 in ten different varieties of cassava was evaluated, which allowed the classification of the cassava varieties TMS60444, SG10735, MCOL1522, MCOL1505 and MCOL2215 as susceptible, while CM6438-14, CM523-7, and MBRA902 were cataloged as resistant. Also, SNPs (Single Nucleotide Polymorphism) polymorphisms were identified in the RXAM1 gene in the same group of cassava varieties. SG10735, CM6438-14, TMS6044, and MBRA685 presented the highest level of polymorphisms, while the varieties CM523-7, CM2177-2 and MCOL1522 were less polymorphic for this gene. The statistical analyses did not allow to identify a significant association between the phenotype and the identified polymorphisms. This study represents a first effort towards the establishment of associating between allelic variants and the cassava response to bacterial blight. La yuca (Manihot esculenta Crantz) es un cultivo importante en regiones del trópico que proporciona alimento para cerca de 1000 millones de personas en todo el mundo. La enfermedad bacteriana más importante es la bacteriosis vascular causada por Xanthomonas axonopodis pv. manihotis (Xam). Recientemente se logró identificar un gen de resistencia denominado RXAM1, el cual codifica para una proteína que posee un dominio LRR (Leucine-Rich Repeat) extracelular y un dominio STK (Serine Threonine Kinase) citoplasmático. RXAM1 colocaliza con un QTL que explica el 13 % de la resistencia a la cepa CIO136 de Xam. En este trabajo se evaluó la respuesta a la infección con la cepa XamCIO136 en diez diferentes variedades de yuca lo cual permitió identificar que las variedades TMS60444, SG10735, MCOL1522, MCOL1505 y MCOL2215 fueron susceptibles, mientras que CM6438-14, CM523-7 y MBRA902 se comportaron como resistentes. Así mismo se identificaron polimorfismos tipo SNPs (Single Nucleotide Polymorphism) en el gen RXAM1 en el mismo grupo de variedades. Las variedades SG10735, CM6438-14, TMS6044 y MBRA685 presentaron el mayor nivel de polimorfismos, mientras que las variedades CM523-7, CM2177-2 y MCOL1522 fueron menos polimórficas para este gen. Los análisis estadísticos no permitieron identificar una asociación significativa entre el fenotipo y los polimorfismos identificados. Este estudio representa un primer esfuerzo con miras a asociar variantes alélicas con el fenotipo de respuesta a la bacteriosis vascular. Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología 2020-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unal.edu.co/index.php/actabiol/article/view/77564 10.15446/abc.v25n2.77564 Acta Biológica Colombiana; Vol. 25 No. 2 (2020); 185 - 193 Acta Biológica Colombiana; Vol. 25 Núm. 2 (2020); 185 - 193 1900-1649 0120-548X spa https://revistas.unal.edu.co/index.php/actabiol/article/view/77564/pdf Derechos de autor 2020 Acta Biológica Colombiana https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNAL
collection OJS
country Colombia
countrycode CO
component Revista
access En linea
databasecode rev-actabol
tag revista
region America del Sur
libraryname Sistema Nacional de Bibliotecas de la UNAL
language spa
format Digital
author Barrera, Andrea del Pilar
Soto-Sedano, Johana
López Carrascal, Camilo Ernesto
spellingShingle Barrera, Andrea del Pilar
Soto-Sedano, Johana
López Carrascal, Camilo Ernesto
Identification of polymorphisms in the Cassava RXAM1 Gene and the association with Cassava Bacterial Blight Resistance
author_facet Barrera, Andrea del Pilar
Soto-Sedano, Johana
López Carrascal, Camilo Ernesto
author_sort Barrera, Andrea del Pilar
title Identification of polymorphisms in the Cassava RXAM1 Gene and the association with Cassava Bacterial Blight Resistance
title_short Identification of polymorphisms in the Cassava RXAM1 Gene and the association with Cassava Bacterial Blight Resistance
title_full Identification of polymorphisms in the Cassava RXAM1 Gene and the association with Cassava Bacterial Blight Resistance
title_fullStr Identification of polymorphisms in the Cassava RXAM1 Gene and the association with Cassava Bacterial Blight Resistance
title_full_unstemmed Identification of polymorphisms in the Cassava RXAM1 Gene and the association with Cassava Bacterial Blight Resistance
title_sort identification of polymorphisms in the cassava rxam1 gene and the association with cassava bacterial blight resistance
description Cassava (Manihot esculenta Crantz) is an essential crop in tropical regions providing food for about one billion people around the world. The most important bacterial disease is cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam). Recently, a resistance gene called RXAM1 was identified, which codes for a protein that has an extracellular LRR (Leucine-Rich Repeat) domain and a cytoplasmic STK (Serine Threonine Kinase) domain. RXAM1 colocalizes with a QTL explaining 13 % of the resistance to the CIO136 strain of Xam. In this work, the response to infection with the strain XamCIO136 in ten different varieties of cassava was evaluated, which allowed the classification of the cassava varieties TMS60444, SG10735, MCOL1522, MCOL1505 and MCOL2215 as susceptible, while CM6438-14, CM523-7, and MBRA902 were cataloged as resistant. Also, SNPs (Single Nucleotide Polymorphism) polymorphisms were identified in the RXAM1 gene in the same group of cassava varieties. SG10735, CM6438-14, TMS6044, and MBRA685 presented the highest level of polymorphisms, while the varieties CM523-7, CM2177-2 and MCOL1522 were less polymorphic for this gene. The statistical analyses did not allow to identify a significant association between the phenotype and the identified polymorphisms. This study represents a first effort towards the establishment of associating between allelic variants and the cassava response to bacterial blight.
publisher Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología
publishDate 2020
url https://revistas.unal.edu.co/index.php/actabiol/article/view/77564
work_keys_str_mv AT barreraandreadelpilar identificationofpolymorphismsinthecassavarxam1geneandtheassociationwithcassavabacterialblightresistance
AT sotosedanojohana identificationofpolymorphismsinthecassavarxam1geneandtheassociationwithcassavabacterialblightresistance
AT lopezcarrascalcamiloernesto identificationofpolymorphismsinthecassavarxam1geneandtheassociationwithcassavabacterialblightresistance
AT barreraandreadelpilar identificaciondepolimorfismosenelgenrxam1deyucaysuasociacionconlaresistenciaalabacteriosisvascular
AT sotosedanojohana identificaciondepolimorfismosenelgenrxam1deyucaysuasociacionconlaresistenciaalabacteriosisvascular
AT lopezcarrascalcamiloernesto identificaciondepolimorfismosenelgenrxam1deyucaysuasociacionconlaresistenciaalabacteriosisvascular
_version_ 1755914059517001728

Similar Items