Biohydrogen production by co-digestion of fruits and vegetable waste and coffee mucilage
In the present investigation, the effects of the substrate composition, organic load, medium acidification time, operation pH, and temperature on the production of hydrogen by anaerobic fermentation with fruits and vegetable waste, and fresh mucilage of coffee was evaluated. For this purpose, tests were carried out in a 20-liter bioreactor operated in batch mode, under a central composite experimental design (CCD). The fermentations were conducted under mesophilic conditions, without adding inoculum, and without sterilizing the substrate. The results for maximum daily hydrogen production (MDP), the maximum hydrogen content in the gas (MHC) and cumulative production (CHP) showed an acceptable fit to second-order polynomial models. All the independent variables were significant, especially the operation pH and the acidification time. Also, the premises for a model obtained by regression, according to error analysis, were fulfilled. In the same way, it was possible to optimize the response variables. The maximum specific production concerning the organic load was 5511 mL H2 gCODremoved-1, and regarding the volatile solids was of 670 mL H2 gVSadded-1. These values are higher than those reported with similar substrates in continuous fermentation, with cell retention, use of inoculum, and substrate pretreatment.
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Universidad Nacional de Colombia - Sede Medellín - Facultad de Ciencias Agrarias
2019
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oai:www.revistas.unal.edu.co:article-731402020-01-02T18:10:16Z Biohydrogen production by co-digestion of fruits and vegetable waste and coffee mucilage Producción de biohidrógeno por co-digestión de residuos de frutas, verduras y mucílago de café Moreno Cárdenas, Edilson León Zapata Zapata, Arley David biofuels fermentation vegetable waste coffee mucilage biocombustible fermentación residuos vegetales mucílago de café In the present investigation, the effects of the substrate composition, organic load, medium acidification time, operation pH, and temperature on the production of hydrogen by anaerobic fermentation with fruits and vegetable waste, and fresh mucilage of coffee was evaluated. For this purpose, tests were carried out in a 20-liter bioreactor operated in batch mode, under a central composite experimental design (CCD). The fermentations were conducted under mesophilic conditions, without adding inoculum, and without sterilizing the substrate. The results for maximum daily hydrogen production (MDP), the maximum hydrogen content in the gas (MHC) and cumulative production (CHP) showed an acceptable fit to second-order polynomial models. All the independent variables were significant, especially the operation pH and the acidification time. Also, the premises for a model obtained by regression, according to error analysis, were fulfilled. In the same way, it was possible to optimize the response variables. The maximum specific production concerning the organic load was 5511 mL H2 gCODremoved-1, and regarding the volatile solids was of 670 mL H2 gVSadded-1. These values are higher than those reported with similar substrates in continuous fermentation, with cell retention, use of inoculum, and substrate pretreatment. En la presente investigación se evaluó el efecto de la composición del sustrato, carga orgánica, tiempo de acidificación del medio, pH de operación y temperatura sobre la producción de hidrógeno por fermentación anaerobia de residuos de frutas, verduras y mucílago fresco de café. Para ello se realizaron pruebas en un bioreactor de 20 L operado en modo batch, bajo un diseño experimental de composición central (CCD). Las fermentaciones fueron realizadas en condiciones mesofílicas, sin adición de inóculo y sin esterilizar el sustrato. Los resultados en producción diaria máxima de hidrógeno (MDP), contenido máximo de hidrógeno en el gas (MHC) y producción acumulada (CHP), presentaron ajuste aceptable a modelos polinomiales de orden dos. Todas las variables independientes fueron significativas, destacándose el pH de operación y el tiempo bajo condiciones ácidas, además se cumplieron las premisas para un modelo obtenido por regresión, según análisis del error. De igual manera fue posible optimizar las variables de respuesta. La máxima producción específica respecto a la carga orgánica fue 5511 mLH2 g-1 DQOremovido y frente a los sólidos volátiles fue 670 mL H2 g-1 SVadicionado, valores superiores a los reportados con sustratos similares en fermentaciones continuas, con retención de células, uso de inóculo y pretratamiento del sustrato. Universidad Nacional de Colombia - Sede Medellín - Facultad de Ciencias Agrarias 2019-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/xml https://revistas.unal.edu.co/index.php/refame/article/view/73140 10.15446/rfnam.v72n3.73140 Revista Facultad Nacional de Agronomía Medellín; Vol. 72 No. 3 (2019); 9007-9018 Revista Facultad Nacional de Agronomía Medellín; Vol. 72 Núm. 3 (2019); 9007-9018 Revista Facultad Nacional de Agronomía Medellín; v. 72 n. 3 (2019); 9007-9018 2248-7026 0304-2847 eng https://revistas.unal.edu.co/index.php/refame/article/view/73140/72108 https://revistas.unal.edu.co/index.php/refame/article/view/73140/72768 Copyright (c) 2019 Revista Facultad Nacional de Agronomía Medellín https://creativecommons.org/licenses/by-nc-sa/4.0 |
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Moreno Cárdenas, Edilson León Zapata Zapata, Arley David |
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Moreno Cárdenas, Edilson León Zapata Zapata, Arley David Biohydrogen production by co-digestion of fruits and vegetable waste and coffee mucilage |
author_facet |
Moreno Cárdenas, Edilson León Zapata Zapata, Arley David |
author_sort |
Moreno Cárdenas, Edilson León |
title |
Biohydrogen production by co-digestion of fruits and vegetable waste and coffee mucilage |
title_short |
Biohydrogen production by co-digestion of fruits and vegetable waste and coffee mucilage |
title_full |
Biohydrogen production by co-digestion of fruits and vegetable waste and coffee mucilage |
title_fullStr |
Biohydrogen production by co-digestion of fruits and vegetable waste and coffee mucilage |
title_full_unstemmed |
Biohydrogen production by co-digestion of fruits and vegetable waste and coffee mucilage |
title_sort |
biohydrogen production by co-digestion of fruits and vegetable waste and coffee mucilage |
description |
In the present investigation, the effects of the substrate composition, organic load, medium acidification time, operation pH, and temperature on the production of hydrogen by anaerobic fermentation with fruits and vegetable waste, and fresh mucilage of coffee was evaluated. For this purpose, tests were carried out in a 20-liter bioreactor operated in batch mode, under a central composite experimental design (CCD). The fermentations were conducted under mesophilic conditions, without adding inoculum, and without sterilizing the substrate. The results for maximum daily hydrogen production (MDP), the maximum hydrogen content in the gas (MHC) and cumulative production (CHP) showed an acceptable fit to second-order polynomial models. All the independent variables were significant, especially the operation pH and the acidification time. Also, the premises for a model obtained by regression, according to error analysis, were fulfilled. In the same way, it was possible to optimize the response variables. The maximum specific production concerning the organic load was 5511 mL H2 gCODremoved-1, and regarding the volatile solids was of 670 mL H2 gVSadded-1. These values are higher than those reported with similar substrates in continuous fermentation, with cell retention, use of inoculum, and substrate pretreatment. |
publisher |
Universidad Nacional de Colombia - Sede Medellín - Facultad de Ciencias Agrarias |
publishDate |
2019 |
url |
https://revistas.unal.edu.co/index.php/refame/article/view/73140 |
work_keys_str_mv |
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