Effect of Dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis

Effect of Dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis

In order to conserve sugarcane germplasm, produce microbial disease-free material, detect resistance to diseases and pests, etc., in vitro propagation of sugarcane has been established in many commercial varieties. In this sense, the aim of this work was to analyze the efficiency of 2,4-dichlorophenoxyacetic acid (2,4D) and 3,6-dichloro -2- methoxybenzoic acid (Dicamba) to induce somatic embryogenesis and regeneration of plantlets from sugarcane varieties C26670, RB855546, V99245, V756, V781, V0050, CC8592, CC8475. For induction of embryogenic callus, leaf discs in tillering stage of 1 cm diameter and 2 mm thick, were inoculated on Murashige-Skoog, 1962 medium (MS), supplemented with 50 ml.l-1 coconut water, 30 g.l-1sucrose and two different treatments: 3 mg.l-1 2,4D or 6.63 mg.l-1 Dicamba, both of them in total darkness at 25 °C, during 1 month. For plant regeneration, embryogenic calli were transferred to ½ MS salts supplemented with coconut water 200 ml.l-1 and sucrose 60 g.l-1 and incubated under continuous light, 25 °C, for 2 months. The highest percent of embryogenic callus induction was obtained in media supplemented with Dicamba, an average of 70.83 % of embryogenic callus by variety, while in media with 2,4-D, 62.08 % of embryogenic callus was obtained by variety. An average of 89,00 % of plantlets was obtained from calli induced on media with Dicamba and an average of 66.12% of plantlets was obtained from calli induced on media supplemented with 2,4D. Using Dicamba it was possible to establish an efficient somatic embryogenesis protocol for these sugarcane varieties.Key words: in vitro culture, auxins, vitroplantlets, Sacharum spp, somatic embryos.

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Main Authors: Alvez, Beatriz, Oropeza, Maira
Format: Digital revista
Language:spa
Published: Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología 2015
Online Access:https://revistas.unal.edu.co/index.php/biotecnologia/article/view/54280
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record_format ojs
institution UNAL
collection OJS
country Colombia
countrycode CO
component Revista
access En linea
databasecode rev-biotecnologia-co
tag revista
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libraryname Sistema Nacional de Bibliotecas de la UNAL
language spa
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author Alvez, Beatriz
Oropeza, Maira
spellingShingle Alvez, Beatriz
Oropeza, Maira
Effect of Dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis
author_facet Alvez, Beatriz
Oropeza, Maira
author_sort Alvez, Beatriz
title Effect of Dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis
title_short Effect of Dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis
title_full Effect of Dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis
title_fullStr Effect of Dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis
title_full_unstemmed Effect of Dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis
title_sort effect of dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis
description In order to conserve sugarcane germplasm, produce microbial disease-free material, detect resistance to diseases and pests, etc., in vitro propagation of sugarcane has been established in many commercial varieties. In this sense, the aim of this work was to analyze the efficiency of 2,4-dichlorophenoxyacetic acid (2,4D) and 3,6-dichloro -2- methoxybenzoic acid (Dicamba) to induce somatic embryogenesis and regeneration of plantlets from sugarcane varieties C26670, RB855546, V99245, V756, V781, V0050, CC8592, CC8475. For induction of embryogenic callus, leaf discs in tillering stage of 1 cm diameter and 2 mm thick, were inoculated on Murashige-Skoog, 1962 medium (MS), supplemented with 50 ml.l-1 coconut water, 30 g.l-1sucrose and two different treatments: 3 mg.l-1 2,4D or 6.63 mg.l-1 Dicamba, both of them in total darkness at 25 °C, during 1 month. For plant regeneration, embryogenic calli were transferred to ½ MS salts supplemented with coconut water 200 ml.l-1 and sucrose 60 g.l-1 and incubated under continuous light, 25 °C, for 2 months. The highest percent of embryogenic callus induction was obtained in media supplemented with Dicamba, an average of 70.83 % of embryogenic callus by variety, while in media with 2,4-D, 62.08 % of embryogenic callus was obtained by variety. An average of 89,00 % of plantlets was obtained from calli induced on media with Dicamba and an average of 66.12% of plantlets was obtained from calli induced on media supplemented with 2,4D. Using Dicamba it was possible to establish an efficient somatic embryogenesis protocol for these sugarcane varieties.Key words: in vitro culture, auxins, vitroplantlets, Sacharum spp, somatic embryos.
publisher Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología
publishDate 2015
url https://revistas.unal.edu.co/index.php/biotecnologia/article/view/54280
work_keys_str_mv AT alvezbeatriz effectofdicambaand24dichlorophenoxiaceticacidonsugarcanesomaticembryogenesis
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spelling oai:www.revistas.unal.edu.co:article-542802016-08-29T21:07:13Z Effect of Dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis Efecto de Dicamba y de ácido 2,4 diclorofenoxiacético sobre la embriogénesis somática en caña de azúcar Alvez, Beatriz Oropeza, Maira Cultivo in vitro auxinas vitroplántulas Sacharum spp. embriogénesis somática auxins vitroplantlets somatic embryos In order to conserve sugarcane germplasm, produce microbial disease-free material, detect resistance to diseases and pests, etc., in vitro propagation of sugarcane has been established in many commercial varieties. In this sense, the aim of this work was to analyze the efficiency of 2,4-dichlorophenoxyacetic acid (2,4D) and 3,6-dichloro -2- methoxybenzoic acid (Dicamba) to induce somatic embryogenesis and regeneration of plantlets from sugarcane varieties C26670, RB855546, V99245, V756, V781, V0050, CC8592, CC8475. For induction of embryogenic callus, leaf discs in tillering stage of 1 cm diameter and 2 mm thick, were inoculated on Murashige-Skoog, 1962 medium (MS), supplemented with 50 ml.l-1 coconut water, 30 g.l-1sucrose and two different treatments: 3 mg.l-1 2,4D or 6.63 mg.l-1 Dicamba, both of them in total darkness at 25 °C, during 1 month. For plant regeneration, embryogenic calli were transferred to ½ MS salts supplemented with coconut water 200 ml.l-1 and sucrose 60 g.l-1 and incubated under continuous light, 25 °C, for 2 months. The highest percent of embryogenic callus induction was obtained in media supplemented with Dicamba, an average of 70.83 % of embryogenic callus by variety, while in media with 2,4-D, 62.08 % of embryogenic callus was obtained by variety. An average of 89,00 % of plantlets was obtained from calli induced on media with Dicamba and an average of 66.12% of plantlets was obtained from calli induced on media supplemented with 2,4D. Using Dicamba it was possible to establish an efficient somatic embryogenesis protocol for these sugarcane varieties.Key words: in vitro culture, auxins, vitroplantlets, Sacharum spp, somatic embryos. Título en ingles: Efecto de Dicamba y de ácido 2,4 diclorofenoxiacético sobre la embriogénesis somática en caña de azúcarEl cultivo in vitro de la caña de azúcar ha sido establecido en muchas variedades comerciales con el propósito de producir material libre de enfermedades microbianas, conservar germoplasma, detectar resistencia a enfermedades y plagas, etc. En este sentido, el objetivo de este trabajo fue analizar la efectividad de las auxinas sintéticas ácido 2,4-diclorofenoxiacético (2,4D) y ácido 3,6-dicloro-2-metoxibenzoico (Dicamba), en la inducción del proceso de embriogénesis somática y la regeneración de vitroplántulas de distintas variedades de caña de azúcar (C26670, RB855546, V99245, V756, V781, V0050, CC8592, CC8475). Para esto se cultivaron discos de hojas en fase de macollamiento, de 1 cm de diámetro y 2 mm de grosor, en medio Murashige-Skoog, 1962 (MS) suplementado con 50 ml.l-1 agua de coco, 30 g.l-1 sacarosa y dos tratamientos diferentes: 3 mg.l-1 2,4-D ó 6.63 mg.l-1 Dicamba, ambos en completa oscuridad a 25ºC, durante 1 mes. Los callos obtenidos se colocaron en medio de regeneración, conteniendo ½ sales MS, 200 ml.l-1 agua de coco y 60 g.L-1 sacarosa, incubándose bajo luz continua, 25ºC, por 2 meses. El mayor porcentaje de callo embriogénico se obtuvo en medios suplementados con Dicamba un promedio de 70,83 % de callo embriogénico por variedad ; mientras que en los medios con 2,4D se obtuvo 62,08 % de callo embriogénico por variedad. Se obtuvo un promedio de 89,00 % de plantas regeneradas a partir de los callos obtenidos en medios con Dicamba y 66,12 % de plantas a partir de callos obtenidos en medios con 2,4D. Con el uso de Dicamba se estableció un sistema eficiente de embriogénesis somática para estas variedades de caña de azúcar. Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología 2015-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf text/html https://revistas.unal.edu.co/index.php/biotecnologia/article/view/54280 10.15446/rev.colomb.biote.v17n2.54280 Revista Colombiana de Biotecnología; Vol. 17 No. 2 (2015); 85-94 Revista Colombiana de Biotecnología; Vol. 17 Núm. 2 (2015); 85-94 1909-8758 0123-3475 spa https://revistas.unal.edu.co/index.php/biotecnologia/article/view/54280/53593 https://revistas.unal.edu.co/index.php/biotecnologia/article/view/54280/53711 https://revistas.unal.edu.co/index.php/biotecnologia/article/view/54280/54114 Derechos de autor 2015 Revista Colombiana de Biotecnología https://creativecommons.org/licenses/by/4.0

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