Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis

Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis

Stevia rebaudiana (Asteraceae) is a plant of economic importance because of its medicinal properties and the presence of sweetener compounds on its leaves. These compounds can be a substitute for sucrose in a wide variety of products used by persons with diabetes and obesity problems. To standardize an efficient and effective propagation method for the different Stevia genotypes grown in Colombia, this study evaluated the effect of different combinations of the plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 6-(gamma, gamma-dimethylallylamino) purine (2iP) and Zeatin on the induction and development of somatic embryos. Adenine and coconut water were also evaluated as supplements in the basal culture medium Murashige and Skoog Basal Salt Mixture (MS) with glutamine. The combination of 2,4-D (18.09 µM) and 2iP (7.38 µM) produced the highest number of somatic embryos per explant, which had well-defined characteristics. The genotype showed a significant effect on the embryogenic response. In the "SRQ-93" genotype, the formation and development of somatic embryos was achieved, whereas the genotypes "Bertoni" and "Morita II" only yielded embryogenic and non-embryogenic calli, respectively. The conversion to seedlings was achieved on the regeneration medium containing gibberellic acid (GA3) (0.29 µM) and activated charcoal.

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Main Authors: Naranjo, Esther Julia, Fernandez Betin, Osman, Urrea Trujillo, Aura Inés, Callejas Posada, Ricardo, Atehortúa Garcés, Lucía
Format: Digital revista
Language:eng
Published: Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología 2016
Online Access:https://revistas.unal.edu.co/index.php/actabiol/article/view/47382
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institution UNAL
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countrycode CO
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access En linea
databasecode rev-actabol
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language eng
format Digital
author Naranjo, Esther Julia
Fernandez Betin, Osman
Urrea Trujillo, Aura Inés
Callejas Posada, Ricardo
Atehortúa Garcés, Lucía
spellingShingle Naranjo, Esther Julia
Fernandez Betin, Osman
Urrea Trujillo, Aura Inés
Callejas Posada, Ricardo
Atehortúa Garcés, Lucía
Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis
author_facet Naranjo, Esther Julia
Fernandez Betin, Osman
Urrea Trujillo, Aura Inés
Callejas Posada, Ricardo
Atehortúa Garcés, Lucía
author_sort Naranjo, Esther Julia
title Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis
title_short Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis
title_full Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis
title_fullStr Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis
title_full_unstemmed Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis
title_sort effect of genotype on the in vitro regeneration of stevia rebaudiana via somatic embryogenesis
description Stevia rebaudiana (Asteraceae) is a plant of economic importance because of its medicinal properties and the presence of sweetener compounds on its leaves. These compounds can be a substitute for sucrose in a wide variety of products used by persons with diabetes and obesity problems. To standardize an efficient and effective propagation method for the different Stevia genotypes grown in Colombia, this study evaluated the effect of different combinations of the plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 6-(gamma, gamma-dimethylallylamino) purine (2iP) and Zeatin on the induction and development of somatic embryos. Adenine and coconut water were also evaluated as supplements in the basal culture medium Murashige and Skoog Basal Salt Mixture (MS) with glutamine. The combination of 2,4-D (18.09 µM) and 2iP (7.38 µM) produced the highest number of somatic embryos per explant, which had well-defined characteristics. The genotype showed a significant effect on the embryogenic response. In the "SRQ-93" genotype, the formation and development of somatic embryos was achieved, whereas the genotypes "Bertoni" and "Morita II" only yielded embryogenic and non-embryogenic calli, respectively. The conversion to seedlings was achieved on the regeneration medium containing gibberellic acid (GA3) (0.29 µM) and activated charcoal.
publisher Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología
publishDate 2016
url https://revistas.unal.edu.co/index.php/actabiol/article/view/47382
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spelling oai:www.revistas.unal.edu.co:article-473822017-07-17T22:32:56Z Effect of genotype on the in vitro regeneration of Stevia rebaudiana via somatic embryogenesis Efecto del genotipo sobre la regeneración in vitro de Stevia rebaudiana a través de embriogénesis somática Naranjo, Esther Julia Fernandez Betin, Osman Urrea Trujillo, Aura Inés Callejas Posada, Ricardo Atehortúa Garcés, Lucía In vitro propagation natural sweetener somatic embryo 2 4-dichlorophenoxyacetic acid (2 4-D) 2-isopentenyl adenine (2iP) in vitro propagation natural sweetener somatic embryo 2,4-dichlorophenoxyacetic acid (2,4-D) 2-isopentenyl adenine (2iP).ácido 2,4-diclorofenoxiacético (2,4-D) embriones somáticos endulzante natural propagación in vitro 2-isopenteniladenina (2iP). Biologia Stevia rebaudiana (Asteraceae) is a plant of economic importance because of its medicinal properties and the presence of sweetener compounds on its leaves. These compounds can be a substitute for sucrose in a wide variety of products used by persons with diabetes and obesity problems. To standardize an efficient and effective propagation method for the different Stevia genotypes grown in Colombia, this study evaluated the effect of different combinations of the plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 6-(gamma, gamma-dimethylallylamino) purine (2iP) and Zeatin on the induction and development of somatic embryos. Adenine and coconut water were also evaluated as supplements in the basal culture medium Murashige and Skoog Basal Salt Mixture (MS) with glutamine. The combination of 2,4-D (18.09 µM) and 2iP (7.38 µM) produced the highest number of somatic embryos per explant, which had well-defined characteristics. The genotype showed a significant effect on the embryogenic response. In the "SRQ-93" genotype, the formation and development of somatic embryos was achieved, whereas the genotypes "Bertoni" and "Morita II" only yielded embryogenic and non-embryogenic calli, respectively. The conversion to seedlings was achieved on the regeneration medium containing gibberellic acid (GA3) (0.29 µM) and activated charcoal. Stevia rebaudiana (Asteraceae), es una planta de gran importancia económica debido a sus propiedades medicinales y a la presencia de compuestos endulzantes en sus hojas, los cuales pueden sustituir la sacarosa en gran variedad de productos utilizados por personas con problemas de diabetes y obesidad. Con el propósito de estandarizar un método de propagación eficiente y efectivo para diferentes genotipos de Stevia cultivados en Colombia, en la presente investigación se evaluó el efecto sobre la inducción y desarrollo de embriones somáticos de diferentes combinaciones de los reguladores de crecimiento vegetal 2,4-D, IAA, IBA, 2iP y Zeatina, además de los suplementos adenina y agua de coco en el medio de cultivo basal Murashige y Skoog (1962), adicionado con glutamina. Con la combinación 2,4-D (18.09 µM) y 2iP (7.38 µM) se obtuvo el mayor número embriones somáticos por explante con características bien definidas. El genotipo tuvo un efecto significativo en la repuesta embriogénica, en el genotipo "SRQ-93" se logró la formación y el desarrollo de embriones somáticos, mientras que en los genotipos "Bertoni" y "Morita II", solo se obtuvo callo embriogénico y no embriogénico respectivamente. La conversión a plántulas se alcanzó en el medio de regeneración conteniendo GA3 (0.29 µM) y carbón activado. Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología 2016-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.unal.edu.co/index.php/actabiol/article/view/47382 10.15446/abc.v21n1.47382 Acta Biológica Colombiana; Vol. 21 No. 1 (2016); 87-98 Acta Biológica Colombiana; Vol. 21 Núm. 1 (2016); 87-98 1900-1649 0120-548X eng https://revistas.unal.edu.co/index.php/actabiol/article/view/47382/21%281%29pdf_art11 https://revistas.unal.edu.co/index.php/actabiol/article/view/47382/54346 Derechos de autor 2016 Acta Biológica Colombiana https://creativecommons.org/licenses/by/4.0

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