In vitro compatibility of the biopesticide based on Lecanicillium lecanii (Hypocreales: Clavicipitaceae) with agrochemicals used in cotton and eggplant crops

In vitro compatibility of the biopesticide based on Lecanicillium lecanii (Hypocreales: Clavicipitaceae) with agrochemicals used in cotton and eggplant crops

A colombian isolate of Lecanicillium lecanii formulated as dispersible granules WG, has shown high efficiency to control Bemisia tabaci in cotton and eggplant crops. Considering that, the objetive of this work was to determine the in vitrocompatibility of biopesticide based on L. lecanii with agrochemicals (insecticides and fungicides) that are most frequently used in tobacco and eggplant crops. In vitro compatibility of L. lecanii with agrochemicals was determinated by germination (%) and Colony Forming Units (CFU) in the presence of pesticides and also estimating the inhibitory concentration 10 (IC10). Each agrochemical was evaluated at the recommended dose, a half and a quarter of it. For the three doses tested (Benomyl®, Vitavax®, Ridomil® and Manzate®) were not compatible with L. lecanii, because these inhibited germination and Colony Forming Units of the fungus. Confidor® did not inhibit viability compared to control treatment, and it was considered compatible with the biopesticide. When the recommended dose (Oportune®, Actara®, Match® and Malathion®)was used, the germination of the L. lecanii was lower than 80%, then these products were classified as non-compatible with the biopesticide based on L. lecanii. The only agrochemical that was compatible in vitro with L. lecanii was Confidor®. However, is necesary to evaluate the in vivo effect of agrochemicals commonly used by farmers on L. lecanii, in order to develop and establish integrated management strategies for the control of the whitefly Bemisia tabaci.

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Main Authors: Santos Díaz, Adriana Marcela, Grijalba, Erika, Victoria Zuluaga, María, Gómez, Martha, Villamizar, Laura
Format: Digital revista
Language:spa
Published: Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología 2013
Online Access:https://revistas.unal.edu.co/index.php/biotecnologia/article/view/38025
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language spa
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author Santos Díaz, Adriana Marcela
Grijalba, Erika
Victoria Zuluaga, María
Gómez, Martha
Villamizar, Laura
spellingShingle Santos Díaz, Adriana Marcela
Grijalba, Erika
Victoria Zuluaga, María
Gómez, Martha
Villamizar, Laura
In vitro compatibility of the biopesticide based on Lecanicillium lecanii (Hypocreales: Clavicipitaceae) with agrochemicals used in cotton and eggplant crops
author_facet Santos Díaz, Adriana Marcela
Grijalba, Erika
Victoria Zuluaga, María
Gómez, Martha
Villamizar, Laura
author_sort Santos Díaz, Adriana Marcela
title In vitro compatibility of the biopesticide based on Lecanicillium lecanii (Hypocreales: Clavicipitaceae) with agrochemicals used in cotton and eggplant crops
title_short In vitro compatibility of the biopesticide based on Lecanicillium lecanii (Hypocreales: Clavicipitaceae) with agrochemicals used in cotton and eggplant crops
title_full In vitro compatibility of the biopesticide based on Lecanicillium lecanii (Hypocreales: Clavicipitaceae) with agrochemicals used in cotton and eggplant crops
title_fullStr In vitro compatibility of the biopesticide based on Lecanicillium lecanii (Hypocreales: Clavicipitaceae) with agrochemicals used in cotton and eggplant crops
title_full_unstemmed In vitro compatibility of the biopesticide based on Lecanicillium lecanii (Hypocreales: Clavicipitaceae) with agrochemicals used in cotton and eggplant crops
title_sort in vitro compatibility of the biopesticide based on lecanicillium lecanii (hypocreales: clavicipitaceae) with agrochemicals used in cotton and eggplant crops
description A colombian isolate of Lecanicillium lecanii formulated as dispersible granules WG, has shown high efficiency to control Bemisia tabaci in cotton and eggplant crops. Considering that, the objetive of this work was to determine the in vitrocompatibility of biopesticide based on L. lecanii with agrochemicals (insecticides and fungicides) that are most frequently used in tobacco and eggplant crops. In vitro compatibility of L. lecanii with agrochemicals was determinated by germination (%) and Colony Forming Units (CFU) in the presence of pesticides and also estimating the inhibitory concentration 10 (IC10). Each agrochemical was evaluated at the recommended dose, a half and a quarter of it. For the three doses tested (Benomyl®, Vitavax®, Ridomil® and Manzate®) were not compatible with L. lecanii, because these inhibited germination and Colony Forming Units of the fungus. Confidor® did not inhibit viability compared to control treatment, and it was considered compatible with the biopesticide. When the recommended dose (Oportune®, Actara®, Match® and Malathion®)was used, the germination of the L. lecanii was lower than 80%, then these products were classified as non-compatible with the biopesticide based on L. lecanii. The only agrochemical that was compatible in vitro with L. lecanii was Confidor®. However, is necesary to evaluate the in vivo effect of agrochemicals commonly used by farmers on L. lecanii, in order to develop and establish integrated management strategies for the control of the whitefly Bemisia tabaci.
publisher Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología
publishDate 2013
url https://revistas.unal.edu.co/index.php/biotecnologia/article/view/38025
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spelling oai:www.revistas.unal.edu.co:article-380252019-06-06T19:18:45Z In vitro compatibility of the biopesticide based on Lecanicillium lecanii (Hypocreales: Clavicipitaceae) with agrochemicals used in cotton and eggplant crops Compatibilidad in vitro de un bioplaguicida a base de Lecanicillium lecanii (Hypocreales: Clavicipitaceae) con agroquímicos empleados en los cultivos de algodón y berenjena Santos Díaz, Adriana Marcela Grijalba, Erika Victoria Zuluaga, María Gómez, Martha Villamizar, Laura entomopathogen Bemisia tabaci fungicide insecticide entomopatógeno Bemisia tabaci fungicidas insecticidas A colombian isolate of Lecanicillium lecanii formulated as dispersible granules WG, has shown high efficiency to control Bemisia tabaci in cotton and eggplant crops. Considering that, the objetive of this work was to determine the in vitrocompatibility of biopesticide based on L. lecanii with agrochemicals (insecticides and fungicides) that are most frequently used in tobacco and eggplant crops. In vitro compatibility of L. lecanii with agrochemicals was determinated by germination (%) and Colony Forming Units (CFU) in the presence of pesticides and also estimating the inhibitory concentration 10 (IC10). Each agrochemical was evaluated at the recommended dose, a half and a quarter of it. For the three doses tested (Benomyl®, Vitavax®, Ridomil® and Manzate®) were not compatible with L. lecanii, because these inhibited germination and Colony Forming Units of the fungus. Confidor® did not inhibit viability compared to control treatment, and it was considered compatible with the biopesticide. When the recommended dose (Oportune®, Actara®, Match® and Malathion®)was used, the germination of the L. lecanii was lower than 80%, then these products were classified as non-compatible with the biopesticide based on L. lecanii. The only agrochemical that was compatible in vitro with L. lecanii was Confidor®. However, is necesary to evaluate the in vivo effect of agrochemicals commonly used by farmers on L. lecanii, in order to develop and establish integrated management strategies for the control of the whitefly Bemisia tabaci. El aislamiento colombiano de Lecanicillium lecanii Vl026 formulado como un granulado dispersable WG, ha demostrado una alta eficiencia para el control de Bemisia tabaci en los cultivos de algodón y berenjena. Teniendo en cuenta el potencial de este bioinsumo, el objetivo del siguiente fue determinar la compatibilidad in vitro del bioplaguicida a base de L. lecanii con agroquímicos (insecticidas y fungicidas) que se utilizan con mayor frecuencia en dichos cultivos. La compatibilidad in vitrose estableció determinando el porcentaje de germinación (%) y las Unidades Formadoras de Colonia (UFC) en presencia de los plaguicidas y estimando la concentración inhibitoria 10 (CI10). Cada plaguicida se evaluó a la dosis recomendada, a la mitad y a un cuarto de ésta. Los fungicidas químicos (Benomil®, Vitavax®, Ridomil® y Manzate®) no fueron compatibles con L. lecanii, ya que inhibieron la germinación y las Unidades Formadoras de Colonia del hongo en las tres dosis evaluadas. En cuanto a los insecticidas químicos, el producto Confidor® no inhibió la viabilidad en comparación con el tratamiento control, considerándose compatible con el bioplaguicida. Cuando se evaluó la dosis completa de los demás insecticidas (Oportune®, Actara®, Match® y Malathion®) se obtuvieron germinaciones inferiores al 80%, por lo que dichos productos se clasificaron como no compatibles con el bioplaguicida a base de L. lecanii. El único agroquímico que fue compatible en condiciones in vitro con L. lecanii fue el producto Confidor®. Sin embargo, se recomienda evaluar el efecto in vivo de los productos químicos habitualmente utilizados por los agricultores sobre L. lecanii, con el propósito de desarrollar y establecer estrategias de manejo integrado de la mosca blanca Bemisia tabaci. Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología 2013-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf text/html https://revistas.unal.edu.co/index.php/biotecnologia/article/view/38025 10.15446/rev.colomb.biote.v15n2.38025 Revista Colombiana de Biotecnología; Vol. 15 No. 2 (2013); 132-142 Revista Colombiana de Biotecnología; Vol. 15 Núm. 2 (2013); 132-142 1909-8758 0123-3475 spa https://revistas.unal.edu.co/index.php/biotecnologia/article/view/38025/42820 https://revistas.unal.edu.co/index.php/biotecnologia/article/view/38025/43505 https://revistas.unal.edu.co/index.php/biotecnologia/article/view/38025/43808 Derechos de autor 2013 Revista Colombiana de Biotecnología https://creativecommons.org/licenses/by/4.0

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