Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants
Phosphorus is limiting for growth of maize plants, and because of that use of fertilizers like rock phosphate has been proposed. However, direct use of rock phosphate is not recommended because of its low availability, so it is necessary to improve it. In this study, a group of diazotrophic bacteria were evaluated as phosphate-solubilizing bacteria, for their production of indolic compounds and for their effects on growth of maize plants. Strains of the genera Azosporillum, Azotobacter, Rhizobium and Klebsiella, were quantitatively evaluated for solubilization of Ca3(PO4)2 and Rock Phosphate as a single source of phosphorous in SRS culture media. Additionally, the phosphatase enzyme activity was quantified at pH 5.0, 7.0 and 8.0 using p-nitrophenyl phosphate, and production of indolic compounds was determined by colorimetric quantification. The effect of inoculation of bacteria on maize was determined in a completely randomized greenhouse experiment where root and shoot dry weights and phosphorus content were assessed. Results showed that strain C50 produced 107.2 mg.L-1 of available-P after 12 days of fermentation, and AC10 strain had the highest phosphatase activity at pH 8 with 12.7 mg of p-nitrophenol mL.h-1. All strains synthetized indolic compounds, and strain AV5 strain produced the most at 63.03 µg.mL-1. These diazotrophic bacteria increased plant biomass up to 39 % and accumulation of phosphorus by 10%. Hence, use of diazotrophic phosphate-solubilizing bacteria may represent an alternative technology for fertilization systems in maize plants.
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Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología
2013
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López-Ortega, Mónica Del Pilar Criollo-Campos, Paola Jimena Gómez-Vargas, Ruth Milena Camelo-Runsinque, Mauricio Estrada-Bonilla, Germán Garrido-Rubiano, María Fernanda Bonilla-Buitrago, Ruth |
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López-Ortega, Mónica Del Pilar Criollo-Campos, Paola Jimena Gómez-Vargas, Ruth Milena Camelo-Runsinque, Mauricio Estrada-Bonilla, Germán Garrido-Rubiano, María Fernanda Bonilla-Buitrago, Ruth Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| author_facet |
López-Ortega, Mónica Del Pilar Criollo-Campos, Paola Jimena Gómez-Vargas, Ruth Milena Camelo-Runsinque, Mauricio Estrada-Bonilla, Germán Garrido-Rubiano, María Fernanda Bonilla-Buitrago, Ruth |
| author_sort |
López-Ortega, Mónica Del Pilar |
| title |
Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| title_short |
Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| title_full |
Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| title_fullStr |
Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| title_full_unstemmed |
Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| title_sort |
characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| description |
Phosphorus is limiting for growth of maize plants, and because of that use of fertilizers like rock phosphate has been proposed. However, direct use of rock phosphate is not recommended because of its low availability, so it is necessary to improve it. In this study, a group of diazotrophic bacteria were evaluated as phosphate-solubilizing bacteria, for their production of indolic compounds and for their effects on growth of maize plants. Strains of the genera Azosporillum, Azotobacter, Rhizobium and Klebsiella, were quantitatively evaluated for solubilization of Ca3(PO4)2 and Rock Phosphate as a single source of phosphorous in SRS culture media. Additionally, the phosphatase enzyme activity was quantified at pH 5.0, 7.0 and 8.0 using p-nitrophenyl phosphate, and production of indolic compounds was determined by colorimetric quantification. The effect of inoculation of bacteria on maize was determined in a completely randomized greenhouse experiment where root and shoot dry weights and phosphorus content were assessed. Results showed that strain C50 produced 107.2 mg.L-1 of available-P after 12 days of fermentation, and AC10 strain had the highest phosphatase activity at pH 8 with 12.7 mg of p-nitrophenol mL.h-1. All strains synthetized indolic compounds, and strain AV5 strain produced the most at 63.03 µg.mL-1. These diazotrophic bacteria increased plant biomass up to 39 % and accumulation of phosphorus by 10%. Hence, use of diazotrophic phosphate-solubilizing bacteria may represent an alternative technology for fertilization systems in maize plants. |
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Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología |
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2013 |
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https://revistas.unal.edu.co/index.php/biotecnologia/article/view/36303 |
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oai:www.revistas.unal.edu.co:article-363032019-08-27T12:53:11Z Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants Caracterización de bacterias diazotróficas solublizadoras de fosfato como promotoras de crecimiento en plantas de maíz López-Ortega, Mónica Del Pilar Criollo-Campos, Paola Jimena Gómez-Vargas, Ruth Milena Camelo-Runsinque, Mauricio Estrada-Bonilla, Germán Garrido-Rubiano, María Fernanda Bonilla-Buitrago, Ruth phosphorus indolic compounds PGPR maize. fósforo compuestos indólicos PGPR maíz. Phosphorus is limiting for growth of maize plants, and because of that use of fertilizers like rock phosphate has been proposed. However, direct use of rock phosphate is not recommended because of its low availability, so it is necessary to improve it. In this study, a group of diazotrophic bacteria were evaluated as phosphate-solubilizing bacteria, for their production of indolic compounds and for their effects on growth of maize plants. Strains of the genera Azosporillum, Azotobacter, Rhizobium and Klebsiella, were quantitatively evaluated for solubilization of Ca3(PO4)2 and Rock Phosphate as a single source of phosphorous in SRS culture media. Additionally, the phosphatase enzyme activity was quantified at pH 5.0, 7.0 and 8.0 using p-nitrophenyl phosphate, and production of indolic compounds was determined by colorimetric quantification. The effect of inoculation of bacteria on maize was determined in a completely randomized greenhouse experiment where root and shoot dry weights and phosphorus content were assessed. Results showed that strain C50 produced 107.2 mg.L-1 of available-P after 12 days of fermentation, and AC10 strain had the highest phosphatase activity at pH 8 with 12.7 mg of p-nitrophenol mL.h-1. All strains synthetized indolic compounds, and strain AV5 strain produced the most at 63.03 µg.mL-1. These diazotrophic bacteria increased plant biomass up to 39 % and accumulation of phosphorus by 10%. Hence, use of diazotrophic phosphate-solubilizing bacteria may represent an alternative technology for fertilization systems in maize plants. El fósforo es limitante para el crecimiento de plantas de maíz y debido a eso se ha propuesto el uso de fertilizantes como la roca fosfórica. Sin embargo, el uso directo de roca fosfórica no es recomendado por su baja solubilidad, por lo que es necesario mejorarlo. En este estudio, un grupo de bacterias diazotróficas fueron evaluadas como bacterias solubilizadoras de fosfato, productoras de compuestos indólicos y sus efectos sobre el crecimiento de plantas de maíz. Cepas de los géneros Azospirillum, Azotobacter, Rhizobium y Klebsiella fueron evaluadas cuantitativamente en la solubilización de Ca3(PO4)2 y roca fosfórica como única fuente de fósforo en medio de cultivo SRS. Adicionalmente, la actividad de la enzima fosfatasa fue cuantificada a pH 5.0, 7.0 y 8.0 usando p-nitrofenil fosfato y, la producción de compuestos indólicos fue determinada por cuantificación colorimétrica. El efecto de la inoculación de las bacterias sobre plantas de maíz fue determinado en un experimento en invernadero con un diseño completamente al azar donde los pesos secos de raíz y hojas y el contenido de fósforo fueron evaluados. Los resultados mostraron que la cepa C50 produjo 107.2 mg.L-1 de fósforo disponible después de 12 días de fermentación y que la cepa AC10 tuvo la más alta actividad fosfatasa a pH 8 con 12.7 mg de p-nitrofenol mL.h-1. Todas las cepas sintetizaron compuestos indólicos y la cepa AV5 produjo la más alta cantidad con 63.03 µg.mL-1. Estas bacterias diazotróficas incrementaron la biomasa de las plantas por encima del 39 % y de la acumulación de fósforo por el 10 %. Aquí, el uso de bacterias diazotróficas solubilizadoras de fosfato puede representar una alternativa tecnológica para los sistemas de fertilización en plantas de maíz. Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología 2013-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf text/html https://revistas.unal.edu.co/index.php/biotecnologia/article/view/36303 10.15446/rev.colomb.biote.v15n2.36303 Revista Colombiana de Biotecnología; Vol. 15 No. 2 (2013); 115-123 Revista Colombiana de Biotecnología; Vol. 15 Núm. 2 (2013); 115-123 1909-8758 0123-3475 spa https://revistas.unal.edu.co/index.php/biotecnologia/article/view/36303/42813 https://revistas.unal.edu.co/index.php/biotecnologia/article/view/36303/43503 https://revistas.unal.edu.co/index.php/biotecnologia/article/view/36303/43806 Derechos de autor 2013 Revista Colombiana de Biotecnología https://creativecommons.org/licenses/by/4.0 |