In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp

In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp

The objective of this study was to evaluate in vitro inhibitory activity of extracts from leaves of Melissa officinalis, Origanum vulgare, Jatropha gossypilia, Eucalyptus sp. Melia azederach and Mascagnia concinna on isolated fungus of the genus Colletotrichum, which causes anthracnose disease in the crop of yam (Dioscorea alata, D. rotundata) in the department of Sucre, Colombia. To obtain the ethanol extract from leaves of M. officinalis and O. vulgare methods of percolation were used and for the rest of plant species, the Soxhlet method was performed. Once leaf extracts were prepared, evaluation for inhibitory activity on six isolated fungus of the genus Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 and BVC 507) was carried out. Inhibitory to the test method was used for direct seeding on the surface of potato-dextrose agar. On the different strains were added 250 µl of each extract separately. We used a positive control with nystatin (4 mg/ml) and an absolute control without any treatment. The test was evaluated by measuring the radial growth of each strain with different treatments at 96 and 168 h. A randomized complete block with three replications, to test significant differences between treatments, strains and inhibit time was used. The results expressed as percentage of inhibition, showed high activity of extracts obtained from leaves of plants of M. azederach and M. concinna, the fungi evaluated and its action was similar to the positive control.

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Main Authors: Pérez Cordero, Alexander, Rojas Sierra2, Johanna, Chamorro Anaya, Leonardo, Pérez Palencia, Katy
Format: Digital revista
Language:spa
Published: Universidad Nacional de Colombia - Sede Palmira 2011
Online Access:https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27838
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record_format ojs
institution UNAL
collection OJS
country Colombia
countrycode CO
component Revista
access En linea
databasecode rev-actaagronomica
tag revista
region America del Sur
libraryname Sistema Nacional de Bibliotecas de la UNAL
language spa
format Digital
author Pérez Cordero, Alexander
Rojas Sierra2, Johanna
Chamorro Anaya, Leonardo
Pérez Palencia, Katy
spellingShingle Pérez Cordero, Alexander
Rojas Sierra2, Johanna
Chamorro Anaya, Leonardo
Pérez Palencia, Katy
In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp
author_facet Pérez Cordero, Alexander
Rojas Sierra2, Johanna
Chamorro Anaya, Leonardo
Pérez Palencia, Katy
author_sort Pérez Cordero, Alexander
title In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp
title_short In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp
title_full In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp
title_fullStr In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp
title_full_unstemmed In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp
title_sort in vitro evaluation of inhibitory activity of plant extracts on colletotrichum spp
description The objective of this study was to evaluate in vitro inhibitory activity of extracts from leaves of Melissa officinalis, Origanum vulgare, Jatropha gossypilia, Eucalyptus sp. Melia azederach and Mascagnia concinna on isolated fungus of the genus Colletotrichum, which causes anthracnose disease in the crop of yam (Dioscorea alata, D. rotundata) in the department of Sucre, Colombia. To obtain the ethanol extract from leaves of M. officinalis and O. vulgare methods of percolation were used and for the rest of plant species, the Soxhlet method was performed. Once leaf extracts were prepared, evaluation for inhibitory activity on six isolated fungus of the genus Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 and BVC 507) was carried out. Inhibitory to the test method was used for direct seeding on the surface of potato-dextrose agar. On the different strains were added 250 µl of each extract separately. We used a positive control with nystatin (4 mg/ml) and an absolute control without any treatment. The test was evaluated by measuring the radial growth of each strain with different treatments at 96 and 168 h. A randomized complete block with three replications, to test significant differences between treatments, strains and inhibit time was used. The results expressed as percentage of inhibition, showed high activity of extracts obtained from leaves of plants of M. azederach and M. concinna, the fungi evaluated and its action was similar to the positive control.
publisher Universidad Nacional de Colombia - Sede Palmira
publishDate 2011
url https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27838
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spelling oai:www.revistas.unal.edu.co:article-278382014-06-21T23:54:35Z In vitro evaluation of inhibitory activity of plant extracts on Colletotrichum spp Evaluación in vitro de la actividad inhibitoria de extractos vegetales sobre aislados de Colletotrichum spp Pérez Cordero, Alexander Rojas Sierra2, Johanna Chamorro Anaya, Leonardo Pérez Palencia, Katy Anthracnose Colletotrichum pest control plant extracts. Antracnosis Colletotrichum control de enfermedades extractos vegetales. The objective of this study was to evaluate in vitro inhibitory activity of extracts from leaves of Melissa officinalis, Origanum vulgare, Jatropha gossypilia, Eucalyptus sp. Melia azederach and Mascagnia concinna on isolated fungus of the genus Colletotrichum, which causes anthracnose disease in the crop of yam (Dioscorea alata, D. rotundata) in the department of Sucre, Colombia. To obtain the ethanol extract from leaves of M. officinalis and O. vulgare methods of percolation were used and for the rest of plant species, the Soxhlet method was performed. Once leaf extracts were prepared, evaluation for inhibitory activity on six isolated fungus of the genus Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 and BVC 507) was carried out. Inhibitory to the test method was used for direct seeding on the surface of potato-dextrose agar. On the different strains were added 250 µl of each extract separately. We used a positive control with nystatin (4 mg/ml) and an absolute control without any treatment. The test was evaluated by measuring the radial growth of each strain with different treatments at 96 and 168 h. A randomized complete block with three replications, to test significant differences between treatments, strains and inhibit time was used. The results expressed as percentage of inhibition, showed high activity of extracts obtained from leaves of plants of M. azederach and M. concinna, the fungi evaluated and its action was similar to the positive control. Se evaluó la actividad inhibitoria in vitro de extractos de hojas de Melissa officinalis, Origanum vulgare,Jatropha gossypilia, Eucalyptus sp., Melia azederach y Mascagnia concinna sobre aislados de hongo delgénero Colletotrichum, causante de la enfermedad antracnosis en el cultivo del ñame (Dioscorea alata, D.rotundata) en el departamento de Sucre, Colombia. Para la obtención de extractos etanólicos de hojasde M. officinalis y O. vulgare, se utilizó el método de percolación y para las otras especies vegetales, elmétodo de Soxhlet. Una vez preparados los extractos de hojas, se evaluó su actividad inhibitoria sobreseis aislados de hongo del género Colletotrichum (BVC 279a, BVC 279b, BVC 853, BVC 040, BVC 342 yBVC 507). Para la prueba inhibitoria se utilizó el método de siembra directa sobre la superficie del mediopapa-dextrosa-agar. Sobre las diferentes cepas se adicionaron 250 μl de cada extracto por separado. Seutilizó un control positivo con nistatina (4 mg/ml) y un testigo absoluto sin ningún tipo de tratamiento.La prueba se evaluó midiendo el crecimiento radial de cada cepa con los diferentes tratamientos a 96y 168 h. Se utilizó un diseño de bloques completamente al azar con tres repeticiones, para probardiferencias significativas entre tratamientos, cepas (aislados) y tiempo de inhibición. Los resultadosexpresados en porcentaje de inhibición, mostraron una alta actividad de los extractos obtenidos de lashojas de las plantas de M. azederach y M. concinna, sobre los hongos evaluados y su acción fue similaral control positivo. Universidad Nacional de Colombia - Sede Palmira 2011-04-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/msword application/pdf text/html https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27838 Acta Agronómica; Vol. 60 No. 2 (2011); 158-164 Acta Agronómica; Vol. 60 Núm. 2 (2011); 158-164 Acta Agronómica; v. 60 n. 2 (2011); 158-164 2323-0118 0120-2812 spa https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27838/28107 https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27838/28108 https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/27838/28109 Derechos de autor 2011 Acta Agronómica https://creativecommons.org/licenses/by-nc-nd/4.0

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