Heterotrimeric G-protein subunit Gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets
Abstract: Gαi2 , a heterotrimeric G-protein subunit, regulates various cell functions including ion channel activity, cell differentiation, proliferation and apoptosis. Platelet-expressed Gαi2 is decisive for the extent of tissue injury following ischemia/reperfusion. However, it is not known whether Gαi2 plays a role in the regulation of platelet apoptosis, which is characterized by caspase activation, cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) translocation to the platelet surface. Stimulators of platelet apoptosis include thrombin and collagen-related peptide (CoRP), which are further known to enhance degranulation and activation of αIIb β3-integrin and caspases. Using FACS analysis, we examined the impact of agonist treatment on activation and apoptosis in platelets drawn from mice lacking Gαi2 and their wild-type (WT) littermates. As a result, treatment with either thrombin (0.01 U/mL) or CoRP (2 μg/mL or 5 μg/mL) significantly upregulated PS-exposure and significantly decreased forward scatter, reflecting cell size, in both genotypes. Exposure to CoRP triggered a significant increase in active caspase 3, ceramide formation, surface P-selectin, and αIIb β3-integrin activation. These molecular alterations were significantly less pronounced in Gαi2 -deficient platelets as compared to WT platelets. In conclusion, our data highlight a previously unreported role of Gαi2 signaling in governing platelet activation and apoptosis.
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Wiley Open Access
2018
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Subjects: | APOPTOSIS, CELULAS DE LA SANGRE, REPERFUSION MIOCARDICA, |
Online Access: | https://repositorio.uca.edu.ar/handle/123456789/8687 |
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oai:ucacris:123456789-86872019-09-05T04:14:13Z Heterotrimeric G-protein subunit Gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets Cao, Hang Qadri, Syed M. Lang, Elisabeth Pelzl, Lisann Umbach, Anja T. Leiss, Veronika Birnbaumer, Lutz Nürnberg, Bernd Pieske, Burkert Voelkl, Jakob Gawaz, Meinrad Bissinger, Rosi Lang, Florian APOPTOSIS CELULAS DE LA SANGRE REPERFUSION MIOCARDICA Abstract: Gαi2 , a heterotrimeric G-protein subunit, regulates various cell functions including ion channel activity, cell differentiation, proliferation and apoptosis. Platelet-expressed Gαi2 is decisive for the extent of tissue injury following ischemia/reperfusion. However, it is not known whether Gαi2 plays a role in the regulation of platelet apoptosis, which is characterized by caspase activation, cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) translocation to the platelet surface. Stimulators of platelet apoptosis include thrombin and collagen-related peptide (CoRP), which are further known to enhance degranulation and activation of αIIb β3-integrin and caspases. Using FACS analysis, we examined the impact of agonist treatment on activation and apoptosis in platelets drawn from mice lacking Gαi2 and their wild-type (WT) littermates. As a result, treatment with either thrombin (0.01 U/mL) or CoRP (2 μg/mL or 5 μg/mL) significantly upregulated PS-exposure and significantly decreased forward scatter, reflecting cell size, in both genotypes. Exposure to CoRP triggered a significant increase in active caspase 3, ceramide formation, surface P-selectin, and αIIb β3-integrin activation. These molecular alterations were significantly less pronounced in Gαi2 -deficient platelets as compared to WT platelets. In conclusion, our data highlight a previously unreported role of Gαi2 signaling in governing platelet activation and apoptosis. 2019-09-04T22:19:12Z 2019-09-04T22:19:12Z 2018 Artículo Cao H, Qadri SM, Lang E, et al. Heterotrimeric G-protein subunit Gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets [en línea]. Physiological Reports. 2018;6(17):e13841. doi:10.14814/phy2.13841 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8687 2051-817X https://repositorio.uca.edu.ar/handle/123456789/8687 10.14814/phy2.13841 30187671 eng Acceso Abierto http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Open Access American Physiological Society Physiological Society Physiological Reports. 2018;6(17):e13841 |
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APOPTOSIS CELULAS DE LA SANGRE REPERFUSION MIOCARDICA APOPTOSIS CELULAS DE LA SANGRE REPERFUSION MIOCARDICA |
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APOPTOSIS CELULAS DE LA SANGRE REPERFUSION MIOCARDICA APOPTOSIS CELULAS DE LA SANGRE REPERFUSION MIOCARDICA Cao, Hang Qadri, Syed M. Lang, Elisabeth Pelzl, Lisann Umbach, Anja T. Leiss, Veronika Birnbaumer, Lutz Nürnberg, Bernd Pieske, Burkert Voelkl, Jakob Gawaz, Meinrad Bissinger, Rosi Lang, Florian Heterotrimeric G-protein subunit Gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets |
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Abstract: Gαi2 , a heterotrimeric G-protein subunit, regulates various cell functions including ion channel activity, cell differentiation, proliferation and apoptosis. Platelet-expressed Gαi2 is decisive for the extent of tissue injury following ischemia/reperfusion. However, it is not known whether Gαi2 plays a role in the regulation of platelet apoptosis, which is characterized by caspase activation, cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) translocation to the platelet surface. Stimulators of platelet apoptosis include thrombin and collagen-related peptide (CoRP), which are further known to enhance degranulation and activation of αIIb β3-integrin and caspases. Using FACS analysis, we examined the impact of agonist treatment on activation and apoptosis in platelets drawn from mice lacking Gαi2 and their wild-type (WT) littermates. As a result, treatment with either thrombin (0.01 U/mL) or CoRP (2 μg/mL or 5 μg/mL) significantly upregulated PS-exposure and significantly decreased forward scatter, reflecting cell size, in both genotypes. Exposure to CoRP triggered a significant increase in active caspase 3, ceramide formation, surface P-selectin, and αIIb β3-integrin activation. These molecular alterations were significantly less pronounced in Gαi2 -deficient platelets as compared to WT platelets. In conclusion, our data highlight a previously unreported role of Gαi2 signaling in governing platelet activation and apoptosis. |
format |
Artículo |
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APOPTOSIS CELULAS DE LA SANGRE REPERFUSION MIOCARDICA |
author |
Cao, Hang Qadri, Syed M. Lang, Elisabeth Pelzl, Lisann Umbach, Anja T. Leiss, Veronika Birnbaumer, Lutz Nürnberg, Bernd Pieske, Burkert Voelkl, Jakob Gawaz, Meinrad Bissinger, Rosi Lang, Florian |
author_facet |
Cao, Hang Qadri, Syed M. Lang, Elisabeth Pelzl, Lisann Umbach, Anja T. Leiss, Veronika Birnbaumer, Lutz Nürnberg, Bernd Pieske, Burkert Voelkl, Jakob Gawaz, Meinrad Bissinger, Rosi Lang, Florian |
author_sort |
Cao, Hang |
title |
Heterotrimeric G-protein subunit Gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets |
title_short |
Heterotrimeric G-protein subunit Gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets |
title_full |
Heterotrimeric G-protein subunit Gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets |
title_fullStr |
Heterotrimeric G-protein subunit Gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets |
title_full_unstemmed |
Heterotrimeric G-protein subunit Gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets |
title_sort |
heterotrimeric g-protein subunit gαi2 contributes to agonist-sensitive apoptosis and degranulation in murine platelets |
publisher |
Wiley Open Access |
publishDate |
2018 |
url |
https://repositorio.uca.edu.ar/handle/123456789/8687 |
work_keys_str_mv |
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