Normal development of spinal axons in early embryo stages and posterior locomotor function is independent of GAL-1
Abstract: It was recently described that Galectin-1 (Gal-1) promotes axonal growth after spinal cord injury. This effect depends on protein dimerization, since monomeric Gal-1 fails to stimulate axonal re-growth. Gal-1 is expressed in vivo at concentrations that favor the monomeric species. The aim of the present study is to investigate whether endogenous Gal-1 is required for spinal axon development and normal locomotor behavior in mice. In order to characterize axonal development, we used a novel combination of 3-DISCO technique with 1-photon microscopy and epifluorescence microscopy under high power LED illumination, followed by serial image section deconvolution and 3-D reconstruction. Cleared whole lgals-1 -/- embryos were used to analyze the 3-D cytoarchitecture of motor, commissural, and sensory axons. This approach allowed us to evaluate axonal development, including the number of fibers, fluorescence density of the fiber tracts, fiber length as well as the morphology of axonal sprouting, deep within the tissue. Gal-1 deficient embryos did not show morphological/anatomical alterations in any of the axonal populations and parameters analyzed. In addition, specific guidance receptor PlexinA4 did not change its axonal localization in the absence of Gal-1. Finally, Gal-1 deficiency did not change normal locomotor activity in post-natal animals. Taken together, our results show that development of spinal axons as well as the locomotor abilities observed in adult mice are independent of Gal-1. Supporting our previous observations, the present study further validates the use of lgals-1 -/- mice to develop spinal cord- or traumatic brain injury models for the evaluation of the regenerative action of Gal-1.
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Wiley
2017
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Subjects: | PROTEINAS, MEDULA ESPINAL, DESARROLLO EMBRIONARIO, FUNCIONES MOTORAS, |
Online Access: | https://repositorio.uca.edu.ar/handle/123456789/8530 |
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oai:ucacris:123456789-85302020-08-19T00:37:33Z Normal development of spinal axons in early embryo stages and posterior locomotor function is independent of GAL-1 Pasquini, Juana M. Barrantes, Francisco José Quintá, Héctor R. PROTEINAS MEDULA ESPINAL DESARROLLO EMBRIONARIO FUNCIONES MOTORAS Abstract: It was recently described that Galectin-1 (Gal-1) promotes axonal growth after spinal cord injury. This effect depends on protein dimerization, since monomeric Gal-1 fails to stimulate axonal re-growth. Gal-1 is expressed in vivo at concentrations that favor the monomeric species. The aim of the present study is to investigate whether endogenous Gal-1 is required for spinal axon development and normal locomotor behavior in mice. In order to characterize axonal development, we used a novel combination of 3-DISCO technique with 1-photon microscopy and epifluorescence microscopy under high power LED illumination, followed by serial image section deconvolution and 3-D reconstruction. Cleared whole lgals-1 -/- embryos were used to analyze the 3-D cytoarchitecture of motor, commissural, and sensory axons. This approach allowed us to evaluate axonal development, including the number of fibers, fluorescence density of the fiber tracts, fiber length as well as the morphology of axonal sprouting, deep within the tissue. Gal-1 deficient embryos did not show morphological/anatomical alterations in any of the axonal populations and parameters analyzed. In addition, specific guidance receptor PlexinA4 did not change its axonal localization in the absence of Gal-1. Finally, Gal-1 deficiency did not change normal locomotor activity in post-natal animals. Taken together, our results show that development of spinal axons as well as the locomotor abilities observed in adult mice are independent of Gal-1. Supporting our previous observations, the present study further validates the use of lgals-1 -/- mice to develop spinal cord- or traumatic brain injury models for the evaluation of the regenerative action of Gal-1. 2019-08-01T01:37:30Z 2019-08-01T01:37:30Z 2017 Artículo Pasquini, J. M., Barrantes, F. J, Quintá, H. R. Normal development of spinal axons in early embryo stages and posterior locomotor function is independent of GAL-1 [en línea]. Journal of Comparative Neurology. 2017, 525 (13). doi:10.1002/cne.24243. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8530 1096-9861 (online) 0021-9967 (Impreso) https://repositorio.uca.edu.ar/handle/123456789/8530 10.1002/cne.24243 eng Acceso Abierto. 1 año de embargo https://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Journal of Comparative Neurology. 2017, 525 (13) |
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PROTEINAS MEDULA ESPINAL DESARROLLO EMBRIONARIO FUNCIONES MOTORAS PROTEINAS MEDULA ESPINAL DESARROLLO EMBRIONARIO FUNCIONES MOTORAS |
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PROTEINAS MEDULA ESPINAL DESARROLLO EMBRIONARIO FUNCIONES MOTORAS PROTEINAS MEDULA ESPINAL DESARROLLO EMBRIONARIO FUNCIONES MOTORAS Pasquini, Juana M. Barrantes, Francisco José Quintá, Héctor R. Normal development of spinal axons in early embryo stages and posterior locomotor function is independent of GAL-1 |
description |
Abstract: It was recently described that Galectin-1 (Gal-1) promotes axonal growth after spinal cord injury. This effect depends on protein dimerization, since monomeric Gal-1 fails to stimulate axonal re-growth. Gal-1 is expressed in vivo at concentrations that favor the monomeric species. The aim of the present study is to investigate whether endogenous Gal-1 is required for spinal axon development and normal locomotor behavior in mice. In order to characterize axonal development, we used a novel combination of 3-DISCO technique with 1-photon microscopy and epifluorescence microscopy under high power LED illumination, followed by serial image section deconvolution and 3-D reconstruction. Cleared whole lgals-1 -/- embryos were used to analyze the 3-D cytoarchitecture of motor, commissural, and sensory axons. This approach allowed us to evaluate axonal development, including the number of fibers, fluorescence density of the fiber tracts, fiber length as well as the morphology of axonal sprouting, deep within the tissue. Gal-1 deficient embryos did not show morphological/anatomical alterations in any of the axonal populations and parameters analyzed. In addition, specific guidance receptor PlexinA4 did not change its axonal localization in the absence of Gal-1. Finally, Gal-1 deficiency did not change normal locomotor activity in post-natal animals. Taken together, our results show that development of spinal axons as well as the locomotor abilities observed in adult mice are independent of Gal-1. Supporting our previous observations, the present study further validates the use of lgals-1 -/- mice to develop spinal cord- or traumatic brain injury models for the evaluation of the regenerative action of Gal-1. |
format |
Artículo |
topic_facet |
PROTEINAS MEDULA ESPINAL DESARROLLO EMBRIONARIO FUNCIONES MOTORAS |
author |
Pasquini, Juana M. Barrantes, Francisco José Quintá, Héctor R. |
author_facet |
Pasquini, Juana M. Barrantes, Francisco José Quintá, Héctor R. |
author_sort |
Pasquini, Juana M. |
title |
Normal development of spinal axons in early embryo stages and posterior locomotor function is independent of GAL-1 |
title_short |
Normal development of spinal axons in early embryo stages and posterior locomotor function is independent of GAL-1 |
title_full |
Normal development of spinal axons in early embryo stages and posterior locomotor function is independent of GAL-1 |
title_fullStr |
Normal development of spinal axons in early embryo stages and posterior locomotor function is independent of GAL-1 |
title_full_unstemmed |
Normal development of spinal axons in early embryo stages and posterior locomotor function is independent of GAL-1 |
title_sort |
normal development of spinal axons in early embryo stages and posterior locomotor function is independent of gal-1 |
publisher |
Wiley |
publishDate |
2017 |
url |
https://repositorio.uca.edu.ar/handle/123456789/8530 |
work_keys_str_mv |
AT pasquinijuanam normaldevelopmentofspinalaxonsinearlyembryostagesandposteriorlocomotorfunctionisindependentofgal1 AT barrantesfranciscojose normaldevelopmentofspinalaxonsinearlyembryostagesandposteriorlocomotorfunctionisindependentofgal1 AT quintahectorr normaldevelopmentofspinalaxonsinearlyembryostagesandposteriorlocomotorfunctionisindependentofgal1 |
_version_ |
1756275821512753152 |