Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor
Abstract: Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment.
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MDPI AG
2022
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Subjects: | EXTREMOFILO, PSICOFILO, PROTEINASA, PROTEOLITICO, BIORREACTOR, TRANSFERENCIA DE OXIGENO, |
Online Access: | https://repositorio.uca.edu.ar/handle/123456789/16424 |
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oai:ucacris:123456789-164242024-03-26T11:58:47Z Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor Machado, Suellen Feitosa, Valker Pillaca Pullo, Omar Santiago Lario, Luciana Daniela Durães Sette, Lara Pessoa, Adalberto Alves, Harley EXTREMOFILO PSICOFILO PROTEINASA PROTEOLITICO BIORREACTOR TRANSFERENCIA DE OXIGENO Abstract: Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment. 2023-05-30T12:03:31Z 2023-05-30T12:03:31Z 2022 Artículo Machado, S. et al. Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor [en línea]. Bioengineering. 2022, 9 (11). doi: 10.3390/bioengineering9110694. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16424 2306-5354 (online) https://repositorio.uca.edu.ar/handle/123456789/16424 10.3390/bioengineering9110694 eng Acceso abierto Atribución-NoComercial-CompartirIgual 4.0 Internacional http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf MDPI AG Bioengineering. Vol.9, No.11, 2022 |
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EXTREMOFILO PSICOFILO PROTEINASA PROTEOLITICO BIORREACTOR TRANSFERENCIA DE OXIGENO EXTREMOFILO PSICOFILO PROTEINASA PROTEOLITICO BIORREACTOR TRANSFERENCIA DE OXIGENO |
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EXTREMOFILO PSICOFILO PROTEINASA PROTEOLITICO BIORREACTOR TRANSFERENCIA DE OXIGENO EXTREMOFILO PSICOFILO PROTEINASA PROTEOLITICO BIORREACTOR TRANSFERENCIA DE OXIGENO Machado, Suellen Feitosa, Valker Pillaca Pullo, Omar Santiago Lario, Luciana Daniela Durães Sette, Lara Pessoa, Adalberto Alves, Harley Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
description |
Abstract: Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced
from different microorganisms. Microbial proteases have several applications, mainly in the
food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing
and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from
the Antarctic environment and was previously reported to have higher extracellular aspartic protease
production. In addition, advances in the operational conditions of bioreactors for enzyme production
are important to reduce the gap associated with scaling−up processes. This is the first study
that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa
yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud
dextrose broth at 25 °C for 72h under kLa values from 18 to 135 h−1. The results show that kLa (121
h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL)
and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In
conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of
protease expression and secretion influenced by kLa. In this sense, our results could be used for
further industrial investment. |
format |
Artículo |
topic_facet |
EXTREMOFILO PSICOFILO PROTEINASA PROTEOLITICO BIORREACTOR TRANSFERENCIA DE OXIGENO |
author |
Machado, Suellen Feitosa, Valker Pillaca Pullo, Omar Santiago Lario, Luciana Daniela Durães Sette, Lara Pessoa, Adalberto Alves, Harley |
author_facet |
Machado, Suellen Feitosa, Valker Pillaca Pullo, Omar Santiago Lario, Luciana Daniela Durães Sette, Lara Pessoa, Adalberto Alves, Harley |
author_sort |
Machado, Suellen |
title |
Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
title_short |
Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
title_full |
Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
title_fullStr |
Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
title_full_unstemmed |
Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
title_sort |
effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://repositorio.uca.edu.ar/handle/123456789/16424 |
work_keys_str_mv |
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